B7-H4 Protein, Human (HEK293, His, solution)
Based on 1 Customer Validation
The B7-H4 protein acts as a negative regulator, inhibiting T cell-mediated immune responses, including activation, proliferation, cytokine production, and cytotoxicity. When expressed on tumor macrophages, it cooperates with regulatory T cells to suppress tumor-associated antigen-specific T cell immunity. B7-H4 Protein, Human (HEK293, His, solution) is the recombinant human-derived B7-H4 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The B7-H4 protein acts as a negative regulator, inhibiting T cell-mediated immune responses, including activation, proliferation, cytokine production, and cytotoxicity. When expressed on tumor macrophages, it cooperates with regulatory T cells to suppress tumor-associated antigen-specific T cell immunity. B7-H4 Protein, Human (HEK293, His, solution) is the recombinant human-derived B7-H4 protein, expressed by HEK293 , with C-His labeled tag.
Background
B7-H4 protein functions as a negative regulator of the T-cell-mediated immune response, exerting inhibitory effects on T-cell activation, proliferation, cytokine production, and the development of cytotoxicity. Particularly significant when expressed on the cell surface of tumor macrophages, B7-H4, in collaboration with regulatory T-cells (Treg), plays a crucial role in suppressing tumor-associated antigen-specific T-cell immunity. Additionally, B7-H4 is implicated in the promotion of epithelial cell transformation, indicating its multifaceted involvement in modulating immune responses and cellular processes associated with tumor development.
Verified Bioactivity
1.Immobilized Recombinant Human B7-H4 / B7S1 / B7x Protein (His Tag) at 2 μg/mL (100 μL/well) can bind B7-H4 / B7S1 / B7x, Rabbit Pab , the EC50 is 1.5-4 ng/mL.
2.Loaded Felmetatug (HY-P990996) on AHC2 biosensor, can bind B7-H4 Protein, Human (HEK293, His) with an affinity constant of 3.886E-09 M as determined in BLI assay.
3.Loaded Mocertatug (HY-P991703) on ProA biosensor, can bind B7-H4 Protein, Human (HEK293, His, ) with an affinity constant of 1.401E-07 M as determined in BLI assay.
4.Immobilized Recombinant Human B7-H4 / B7S1 / B7x Protein (His Tag) at 1 μg/mL (100 μL/well) can bind Anti-VTCN1(Research Grade FPA-150 Biosimilar), the EC50 is 1.5-5.0 ng/mL.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
Q7Z7D3-1 (F29-A258)
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Gene ID79679
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Molecular Construction
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N-term
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B7-H4 (F29-A258)
Accession # Q7Z7D3-1 -
His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
VTCN1; V-Set Domain Containing T Cell Activation Inhibitor 1; B7-H4; B7H4; B7h.5; B7S1; B7x; V-Set Domain-Containing T-Cell Activation Inhibitor 1; Immune Costimulatory Protein B7-H4; B7 Superfamily Member 1; B7 Family Member, H4; B7 Homolog 4; FLJ22418;
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AA Sequence
FGISGRHSITVTTVASAGNIGEDGILSCTFEPDIKLSDIVIQWLKEGVLGLVHEFKEGKDELSEQDEMFRGRTAVFADQVIVGNASLRLKNVQLTDAGTYKCYIITSKGKGNANLEYKTGAFSMPEVNVDYNASSETLRCEAPRWFPQPTVVWASQVDQGANFSEVSNTSFELNSENVTMKVVSVLYNVTINNTYSCMIENDIAKATGDIKVTESEIKRRSHLQLLNSKA
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Predicted Molecular Mass
26.8 kDa
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Molecular Weight
Approximately 39-55 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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