BSP2 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
BSP2 Protein, Mouse (HEK293, His) is a recombinant mouse bone sialoprotein 2 produced in HEK293 cells. Bone Sialoprotein 2, one of the major bone matrix proteins, with its previously known role in osseous tissue degradation, has been recently proven to play a role in the nucleation of hydroxyl apatite during osseous tissue mineralization.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
BSP2 Protein, Mouse (HEK293, His) is a recombinant mouse bone sialoprotein 2 produced in HEK293 cells. Bone Sialoprotein 2, one of the major bone matrix proteins, with its previously known role in osseous tissue degradation, has been recently proven to play a role in the nucleation of hydroxyl apatite during osseous tissue mineralization[1].
Bone Sialoprotein (BSP) is a member of the "Small Integrin-Binding Ligand N-linked Glycoproteins" (SIBLING) extracellular matrix protein family of mineralized tissues. BSP has been less studied than other SIBLING proteins such as Osteopontin (OPN), which is coexpressed with it in several skeletal cell types[2].
Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast cancer cells. When 5×104 cells/well are added to mouse BSP2 coated plates (3 µg/mL with 100 µL/well), approximately 60.05% will adhere after 30 minutes at 37 °C.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-6*His
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Accession
AAA21726.1 (F17-Q324)
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Molecular Construction
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N-term
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BSP2 (F17-Q324)
Accession # AAA21726.1 -
6*His
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C-term
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Protein Length
Partial
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Synonyms
IBSP; Integrin-Binding Sialoprotein; Integrin Binding Sialoprotein; Cell-Binding Sialoprotein; Bone Sialoprotein II; Bone Sialoprotein 2; BSP-II; BSP II; SP-II; BNSP; BSP; Bone Sialoprotein
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AA Sequence
FSMKNFHRRIKAEDSEENGVFKYRPRYFLYKHAYFYPPLKRFPVQGGSDSSEENGDGDSSEEEGEEEETSNEEENNEDSEGNEDQEAEAENATLSTLSGVTASYGAETTPQAQTFELAALQLPKKAGDAESRAPKVKESDEEEEEEEEEEENENEEAEVDENELAVNGTSTNSTEVDGGNGSSGGDNGEEAEAEEASVTEAGAEGTTGGRELTSVGTQTAVLLNGFQQTTPPPEAYGTTSPPIRKSSTVEYGGEYEQTGNEYNNEYEVYDNENGEPRGDTYRAYEDEYSYYKGHGYEGYEGQNYYYHQ
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Molecular Weight
Approximately 71 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized after extensive dialysis against PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Im BJ, et al. Promotion of osteoblastic differentiation and osteogenic transcription factor expression on a microgroove titanium surface with immobilized fibronectin or bone sialoprotein II. Biomed Mater. 2016;11(3):035020. Published 2016 Jun 21. [Content Brief]
[2]. Bouleftour W, et al. The role of the SIBLING, Bone Sialoprotein in skeletal biology - Contribution of mouse experimental genetics. Matrix Biol. 2016;52-54:60-77. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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