BTK Protein, Human (Active, sf9)
Based on 1 Customer Validation
The BTK protein is a key non-receptor tyrosine kinase that is essential for B lymphocyte development and signaling. After BCR activation, BTK initiates a cascade reaction, phosphorylates PLCG2 and activates downstream pathways, affecting calcium mobilization and PKC activation. BTK, Human (Active, sf9) is the recombinant human-derived BTK protein, expressed by sf9 insect cells, with Tag Free labeled tag.
- Species: Human
- Source: sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The BTK protein is a key non-receptor tyrosine kinase that is essential for B lymphocyte development and signaling. After BCR activation, BTK initiates a cascade reaction, phosphorylates PLCG2 and activates downstream pathways, affecting calcium mobilization and PKC activation. BTK, Human (Active, sf9) is the recombinant human-derived BTK protein, expressed by sf9 insect cells, with Tag Free labeled tag.
Background
BTK Protein, a non-receptor tyrosine kinase, is indispensable for B lymphocyte development, differentiation, and signaling. Upon antigen binding to the B-cell antigen receptor (BCR), BTK triggers a signaling cascade leading to B-cell activation, phosphorylating PLCG2 and initiating downstream pathways involving calcium mobilization and protein kinase C (PKC) activation. Serving as a platform for diverse signaling proteins, BTK is implicated in cytokine receptor signaling pathways and is crucial in innate and adaptive immunity, particularly within the Toll-like receptors (TLR) pathway. It plays a critical role in regulating TLR9 activation in splenic B-cells and induces the activity of NF-kappa-B, contributing to the transcriptional regulation of numerous genes. Additionally, BTK activates NLRP3 inflammasome assembly, phosphorylates transcription factor GTF2I, and is involved in apoptosis regulation, highlighting its multifaceted functions in immune response and cellular processes.
Verified Bioactivity
The activity of TEC is verified by the ADP-Glo method: Incubate TEC protein, ATP, and the substrate at room temperature for 40 minutes, then add the ADP-Glo reagent and read the luminescence signal using the chemiluminescence module of the microplate reader.
Technical Parameters
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Species Human
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Source sf9 insect cells
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Tag Tag Free
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Accession
Q06187-1 (M1-S659)
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Gene ID695
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Molecular Construction
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N-term
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(M1-S659)
Accession # Q06187-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
BTK; Tyrosine-Protein Kinase BTK Isoform (Lacking Exon 13 To 17); Prev. AGMX1; Tyrosine-Protein Kinase BTK Isoform (Lacking Exon 14); Prev. IMD1; Tyrosine-Protein Kinase BTK Isoform 52; ATK; Tyrosine-Protein Kinase BTK Isoform 51; PSCTK1; Tyrosine-Protein
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AA Sequence
MAAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVVPEKNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKKPLPPEPAAAPVSTSELKKVVALYDYMPMNANDLQLRKGDEYFILEESNLPWWRARDKNGQEGYIPSNYVTEAEDSIEMYEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKSTGDPQGVIRHYVVCSTPQSQYYLAEKHLFSTIPELINYHQHNSAGLISRLKYPVSQQNKNAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGKWRGQYDVAIKMIKEGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASEKVYTIMYSCWHEKADERPTFKILLSNILDVMDEES
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Molecular Weight
Approximately 76 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT, 100 mM trehalose.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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