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CCN1/Cyr61 Protein, Mouse (His)

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CCN1/Cyr61 Protein, Mouse (His) is the recombinant mouse-derived CCN1/Cyr61 protein, expressed by E. coli, with N-10*His tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CCN1/Cyr61 Protein, Mouse (His)
  • CCN1/Cyr61 Protein, Mouse (His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

CCN1/Cyr61 Protein, Mouse (His) is the recombinant mouse-derived CCN1/Cyr61 protein, expressed by E. coli, with N-10*His tag.

Background

CCN1/Cyr61 protein plays a multifaceted role in cellular processes, actively promoting cell proliferation, chemotaxis, angiogenesis, and adhesion. It demonstrates a crucial involvement in wound healing by orchestrating the up-regulation of key genes related to angiogenesis, inflammation, and matrix remodeling in skin fibroblasts. CCN1's gene-regulatory effects are heparin-binding dependent. Additionally, it exerts regulatory control by down-regulating the expression of collagen type-1 subunits alpha-1 and alpha-2. The protein facilitates cell adhesion and signaling through integrin alpha-6/beta-1, promotes cell migration via integrin alpha-v/beta-5, and stimulates cell proliferation through integrin alpha-v/beta-3. Its interactions with integrins are context-dependent, relying on heparin and the specific cell type. In skin fibroblasts, CCN1 binds ITGA6/ITGB1, in endothelial cells it binds ITGAV/ITGB3, and in platelets, it associates with ITGA2B/ITGB3, thereby influencing diverse cellular functions.

Verified Bioactivity

Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Recombinant Human Cyr61/CCN1 Fc Chimera immobilized at 1.5 μg/mL, 100 μL/well, will mediate >25 % BALB/3T3 cell adhesion (3 x 104 cell/well added).

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag N-10*His
  • Accession

    P18406 (T25-D379)

  • Gene ID
    16007
  • Molecular Construction
    • N-term
    • 10*His
    • CCN1/Cyr61 (T25-D379)
      Accession # P18406
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    CCN1; Cysteine Rich Angiogenic Inducer 61; Prev. IGFBP10; IGF-Binding Protein 10; Prev. CYR61; IGFBP-10; GIG1; IBP-10; CCN Family Member 1; Cysteine-Rich Heparin-Binding Protein 61; Protein CYR61; Cysteine-Rich, Anigogenic Inducer, 61; Insulin-Like Growth

  • AA Sequence

    TCPAACHCPLEAPKCAPGVGLVRDGCGCCKVCAKQLNEDCSKTQPCDHTKGLECNFGASSTALKGICRAQSEGRPCEYNSRIYQNGESFQPNCKHQCTCIDGAVGCIPLCPQELSLPNLGCPNPRLVKVSGQCCEEWVCDEDSIKDSLDDQDDLLGLDASEVELTRNNELIAIGKGSSLKRLPVFGTEPRVLFNPLHAHGQKCIVQTTSWSQCSKSCGTGISTRVTNDNPECRLVKETRICEVRPCGQPVYSSLKKGKKCSKTKKSPEPVRFTYAGCSSVKKYRPKYCGSCVDGRCCTPLQTRTVKMRFRCEDGEMFSKNVMMIQSCKCNYNCPHPNEASFRLYSLFNDIHKFRD

  • Predicted Molecular Mass

    42.6 kDa

  • Molecular Weight

    Approximately 44 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • CCN1/Cyr61 Protein, Mouse (His) Product Image
    1/16
  • Experimental Validation Results for CCN1/Cyr61 Protein, Mouse (His)
  • CCN1/Cyr61 Protein, Mouse (His) Product Image