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CD163 Protein, Mouse (HEK293, His)

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Based on 1 publication(s) in Google Scholar

The CD163 protein is critical in macrophages for clearance and endocytosis of hemoglobin/haptoglobin complexes, protecting tissues from oxidative damage. It promotes iron recycling by promoting complex endocytosis and heme breakdown, while calcium and pH affect hemoglobin binding. CD163 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD163 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The CD163 protein is critical in macrophages for clearance and endocytosis of hemoglobin/haptoglobin complexes, protecting tissues from oxidative damage. It promotes iron recycling by promoting complex endocytosis and heme breakdown, while calcium and pH affect hemoglobin binding. CD163 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD163 protein, expressed by HEK293 , with C-His labeled tag.

Background

CD163 protein is intricately involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes within macrophages, serving a crucial function in protecting tissues from potential oxidative damage caused by free hemoglobin. Additionally, CD163 may contribute to the uptake and recycling of iron by facilitating the endocytosis of hemoglobin/haptoglobin complexes and subsequent breakdown of heme. The protein exhibits a calcium-dependent and pH-dependent binding affinity for hemoglobin/haptoglobin complexes, initiating a cascade of intracellular signals involving tyrosine kinase-dependent calcium mobilization, inositol triphosphate production, and the secretion of interleukin-6 (IL6) and colony-stimulating factor 1 (CSF1). Furthermore, the soluble form of CD163 (sCD163), released through shedding, may exert an anti-inflammatory role.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-8*His
  • Accession

    Q2VLH6-1 (V39-T1045)

  • Gene ID
    /
  • Molecular Construction
    • N-term
    • CD163 (V39-T1045)
      Accession # Q2VLH6-1
    • 8*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CD163; MM130; CD163 Molecule; Hemoglobin Scavenger Receptor; M130; CD163 Antigen; Scavenger Receptor Cysteine-Rich Type 1 Protein M130; Macrophage-Associated Antigen; SCARI1

  • AA Sequence

    VTNAPGEMKKELRLAGGENNCSGRVELKIHDKWGTVCSNGWSMNEVSVVCQQLGCPTSIKALGWANSSAGSGYIWMDKVSCTGNESALWDCKHDGWGKHNCTHEKDAGVTCSDGSNLEMRLVNSAGHRCLGRVEIKFQGKWGTVCDDNFSKDHASVICKQLGCGSAISFSGSAKLGAGSGPIWLDDLACNGNESALWDCKHRGWGKHNCDHAEDVGVICLEGADLSLRLVDGVSRCSGRLEVRFQGEWGTVCDDNWDLRDASVVCKQLGCPTAISAIGRVNASEGSGQIWLDNISCEGHEATLWECKHQEWGKHYCHHREDAGVTCSDGADLELRLVGGGSRCAGIVEVEIQKLTGKMCSRGWTLADADVVCRQLGCGSALQTQAKIYSKTGATNTWLFPGSCNGNETTFWQCKNWQWGGLSCDNFEEAKVTCSGHREPRLVGGEIPCSGRVEVKHGDVWGSVCDFDLSLEAASVVCRELQCGTVVSILGGAHFGEGSGQIWGEEFQCSGDESHLSLCSVAPPLDRTCTHSRDVSVVCSRYIDIRLAGGESSCEGRVELKTLGAWGPLCSSHWDMEDAHVLCQQLKCGVAQSIPEGAHFGKGAGQVWSHMFHCTGTEEHIGDCLMTALGAPTCSEGQVASVICSGNQSQTLLPCSSLSPVQTTSSTIPKESEVPCIASGQLRLVGGGGRCAGRVEVYHEGSWGTVCDDNWDMTDANVVCKQLDCGVAINATGSAYFGEGAGAIWLDEVICTGKESHIWQCHSHGWGRHNCRHKEDAGVICSEFMSLRLTNEAHKENCTGRLEVFYNGTWGSIGSSNMSPTTVGVVCRQLGCADNGTVKPIPSDKTPSRPMWVDRVQCPKGVDTLWQCPSSPWKQRQASPSSQESWIICDNKIRLQEGHTDCSGRVEIWHKGSWGTVCDDSWDLNDAKVVCKQLGCGQAVKALKEAAFGPGTGPIWLNEIKCRGNESSLWDCPAKPWSHSDCGHKEDASIQCLPKMTSESHHGTGHPT

  • Predicted Molecular Mass

    109.5 kDa

  • Molecular Weight

    Approximately 120-150 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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