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  5. CD6 Protein, Rat (HEK293, His)

CD6 Protein, Rat (HEK293, His)

Cat. No.: HY-P72924
COA Handling Instructions

CD6, a protein, lacks essential conserved residue(s) necessary for propagating feature annotation. CD6 Protein, Rat (HEK293, His) is the recombinant rat-derived CD6 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD6 Protein, Rat (HEK293, His) is 379 a.a., with molecular weight of ~60-90 kDa.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
Free Sample   Apply now
5 μg $35 In-stock
10 μg $50 In-stock
50 μg $115 In-stock
100 μg $170 In-stock
500 μg $425 In-stock
1 mg $680 In-stock
> 1 mg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

CD6, a protein, lacks essential conserved residue(s) necessary for propagating feature annotation. CD6 Protein, Rat (HEK293, His) is the recombinant rat-derived CD6 protein, expressed by HEK293 , with C-His labeled tag. The total length of CD6 Protein, Rat (HEK293, His) is 379 a.a., with molecular weight of ~60-90 kDa.

Background

CD6 lacks conserved residue(s) essential for the propagation of feature annotation.

Biological Activity

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells/well are added to rat CD6-His coated plates (5 μg/mL and 100 μL/well) in the ppresence of 10μg/mL PHA, 72.012% cells adhered specifically after 60 minutes at 37°C.

Species

Rat

Source

HEK293

Tag

C-His

Accession

Q812A4 (L18-N396)

Gene ID
Molecular Construction
N-term
CD6 (L18-N396)
Accession # Q812A4
His
C-term
Synonyms
T-cell differentiation antigen CD6; T12; TP120; CD6
AA Sequence

LPPPAPSDQHRNGSIPNMSSDPEEQLGIRLVNGSSRCSGFVQVLLESWEPVCAGHWNRAATEAACNALGCGDLGNFTHLAPPTSERPPGATSRNTSSSRNTTWAGAPTVRCHGANWQLCKVEDYECSSDRRLVWVTCAENQAVRLVDGGSRCAGRVEMLQHGEWGTVCDDTWDLDDANVVCKQLKCGWAVKALAGLHFTPGQGPIHRDQVNCSGTEAYLWDCPGRPGDQYCGHKEDAGVVCSEHQSWRLTGGVDSCEGQVEVYFRGVWSTVCDSEWYPSEAKVLCQSLGCGSEVDRPKGLPHSLAGRMYYSCKGEELTLSTCSWRFNNSNLCSQSLAARVVCSGSQRHPNLSTSEVPSRVPVTIESSVPVSVKDKDSQN

Molecular Weight

Approximately 60-90 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CD6 Protein, Rat (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD6 Protein, Rat (HEK293, His)
Cat. No.:
HY-P72924
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