CD6 Protein, Rat (HEK293, His)

Customer Review

Based on 1 Customer Validation

CD6, a protein, lacks essential conserved residue(s) necessary for propagating feature annotation. CD6 Protein, Rat (HEK293, His) is the recombinant rat-derived CD6 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

CD6, a protein, lacks essential conserved residue(s) necessary for propagating feature annotation. CD6 Protein, Rat (HEK293, His) is the recombinant rat-derived CD6 protein, expressed by HEK293 , with C-His labeled tag.

Background

CD6 lacks conserved residue(s) essential for the propagation of feature annotation.

Verified Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104 cells/well are added to rat CD6-His coated plates (5 μg/mL and 100 μL/well) in the ppresence of 10μg/mL PHA, 72.012% cells adhered specifically after 60 minutes at 37°C.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • CD6 (L18-N396)
      Accession # Q812A4
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    CD6; Tp120; CD6 Molecule; T12; T-Cell Differentiation Antigen CD6; Alternative Protein CD6; CD6 Antigen; TP120

  • AA Sequence

    LPPPAPSDQHRNGSIPNMSSDPEEQLGIRLVNGSSRCSGFVQVLLESWEPVCAGHWNRAATEAACNALGCGDLGNFTHLAPPTSERPPGATSRNTSSSRNTTWAGAPTVRCHGANWQLCKVEDYECSSDRRLVWVTCAENQAVRLVDGGSRCAGRVEMLQHGEWGTVCDDTWDLDDANVVCKQLKCGWAVKALAGLHFTPGQGPIHRDQVNCSGTEAYLWDCPGRPGDQYCGHKEDAGVVCSEHQSWRLTGGVDSCEGQVEVYFRGVWSTVCDSEWYPSEAKVLCQSLGCGSEVDRPKGLPHSLAGRMYYSCKGEELTLSTCSWRFNNSNLCSQSLAARVVCSGSQRHPNLSTSEVPSRVPVTIESSVPVSVKDKDSQN

  • Molecular Weight

    Approximately 64-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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