CD96 Protein, Mouse (HEK293, His)

The CD96 protein plays a key role in immune cell adhesion and promotes PVR interactions on target cells during inflammation. Its role extends to promoting the adhesion of immune cells after crossing the endothelium. CD96 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD96 protein, expressed by HEK293 , with C-6*His labeled tag.

CD96 Protein emerges as a potential participant in adhesive interactions during the late phase of the immune response, specifically contributing to the adhesion of activated T and NK cells. Its role in promoting NK cell-target adhesion involves interaction with PVR present on target cells, suggesting a function in the engagement of immune cells with diseased cells during inflammation. The proposed scenario implies that CD96 operates after T and NK cells have traversed the endothelium using integrins and selectins, actively participating in cell-cell interactions within inflamed areas. Structurally, CD96 forms homodimers through disulfide linkages and interacts with PVR, underscoring its potential as a key mediator in immune cell adhesion processes. Further exploration of CD96's precise mechanisms and dynamics in immune responses could provide valuable insights into its role in orchestrating cellular interactions during inflammation and immune surveillance.

Mouse

HEK293

C-6*His

Q3U0X8 (V22-M536)

84544  [NCBI]

Extracellular Domain

VWEELFNVGDDVYALPGSDINLTCQTKEKNFLVQMQWSKVTDKNDMIALYHPQYGLYCGQEHACESQVAATETEKGVTNWTLYLRNISSALGGKYECIFTLYPEGIKTTVYNLIVEPYTQDEHNYTIEIETNRTLEIPCFQNTSSEIPPRFTFSWLVEKDGVEEVLFTHHHHVNNSTSFKGRIRLGGDYRLHLSPVQIQDDGRTFSCHLTVNPLKAWKMSTTVKVFAKPEILMTVENSTMDVLGERVFTCLLKNVFPKANITWFIDGRFLQGNEEGIYITNEEKNCSSGFWELKSVLTRMHSGPSQSNNMTAWCMALSPGPRNKMWNTSSQPITVSFDSVIAPTKHLPTVTGSTLGTQPFSDAGVSPTGYLATPSVTIVDENGLTPDATPQTSNSSMTTKDGNYLEASSGTDAKNSSRAAASSKSGSWPFPFTSPPEWHSLPGTSTGPQEPDSPVSWIPSEVHTSAPLDASLAPHDTIISTTTEFPNVLTTANGTTKIDHGPITSIIVNQPSDGM

130-180 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.