CD96 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
The CD96 protein plays a key role in immune cell adhesion and promotes PVR interactions on target cells during inflammation. Its role extends to promoting the adhesion of immune cells after crossing the endothelium. CD96 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD96 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
The CD96 protein plays a key role in immune cell adhesion and promotes PVR interactions on target cells during inflammation. Its role extends to promoting the adhesion of immune cells after crossing the endothelium. CD96 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD96 protein, expressed by HEK293 , with C-6*His labeled tag.
CD96 Protein emerges as a potential participant in adhesive interactions during the late phase of the immune response, specifically contributing to the adhesion of activated T and NK cells. Its role in promoting NK cell-target adhesion involves interaction with PVR present on target cells, suggesting a function in the engagement of immune cells with diseased cells during inflammation. The proposed scenario implies that CD96 operates after T and NK cells have traversed the endothelium using integrins and selectins, actively participating in cell-cell interactions within inflamed areas. Structurally, CD96 forms homodimers through disulfide linkages and interacts with PVR, underscoring its potential as a key mediator in immune cell adhesion processes. Further exploration of CD96's precise mechanisms and dynamics in immune responses could provide valuable insights into its role in orchestrating cellular interactions during inflammation and immune surveillance.
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-6*His
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Accession
Q3U0X8 (V22-M536)
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Molecular Construction
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N-term
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CD96 (V22-M536)
Accession # Q3U0X8 -
6*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
CD96; TACTILE; CD96 Molecule; T Cell Activation, Increased Late Expression; T-Cell Surface Protein Tactile; CD96 Antigen; T Cell-Activated Increased Late Expression Protein; Alternative Protein CD96; Cell Surface Antigen CD96
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AA Sequence
VWEELFNVGDDVYALPGSDINLTCQTKEKNFLVQMQWSKVTDKNDMIALYHPQYGLYCGQEHACESQVAATETEKGVTNWTLYLRNISSALGGKYECIFTLYPEGIKTTVYNLIVEPYTQDEHNYTIEIETNRTLEIPCFQNTSSEIPPRFTFSWLVEKDGVEEVLFTHHHHVNNSTSFKGRIRLGGDYRLHLSPVQIQDDGRTFSCHLTVNPLKAWKMSTTVKVFAKPEILMTVENSTMDVLGERVFTCLLKNVFPKANITWFIDGRFLQGNEEGIYITNEEKNCSSGFWELKSVLTRMHSGPSQSNNMTAWCMALSPGPRNKMWNTSSQPITVSFDSVIAPTKHLPTVTGSTLGTQPFSDAGVSPTGYLATPSVTIVDENGLTPDATPQTSNSSMTTKDGNYLEASSGTDAKNSSRAAASSKSGSWPFPFTSPPEWHSLPGTSTGPQEPDSPVSWIPSEVHTSAPLDASLAPHDTIISTTTEFPNVLTTANGTTKIDHGPITSIIVNQPSDGM
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Predicted Molecular Mass
57.6 kDa
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Molecular Weight
Approximately 120-170 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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