CKMT2 Protein, Rat (His, Solution)

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The CKMT2 protein is predicted to have ubiquitin protein transferase activity and participate in the regulation of the epidermal growth factor receptor signaling pathway. It is involved in autoubiquitination and ubiquitin-dependent protein catabolic processes through the multivesicular body sorting pathway. CKMT2 Protein, Rat (His, Solution) is the recombinant rat-derived CKMT2 protein, expressed by E. coli , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

The CKMT2 protein is predicted to have ubiquitin protein transferase activity and participate in the regulation of the epidermal growth factor receptor signaling pathway. It is involved in autoubiquitination and ubiquitin-dependent protein catabolic processes through the multivesicular body sorting pathway. CKMT2 Protein, Rat (His, Solution) is the recombinant rat-derived CKMT2 protein, expressed by E. coli , with N-His labeled tag.

Background

CKMT2 Protein is predicted to possess ubiquitin-protein transferase activity and is implicated in negative regulation of the epidermal growth factor receptor signaling pathway, protein autoubiquitination, and ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway. The protein is anticipated to be located in the cytosol. Notably, CKMT2 exhibits ubiquitous expression across various tissues, with notable levels observed in the heart, fat, and 25 other tissues, suggesting its involvement in diverse cellular processes and physiological functions throughout the body.

Technical Parameters

  • Species Rat
  • Source E. coli
  • Tag N-His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • His
    • CKMT2 (D40-K419)
      Accession # NP_001121124.1
    • C-term
  • Protein Length

    Partial

  • Synonyms

    CKMT2; Sarcomeric Mitochondrial Creatine Kinase; Creatine Kinase, Mitochondrial 2; Creatine Kinase S-Type, Mitochondrial; SMTCK; S-MtCK; Creatine Kinase, Mitochondrial 2 (Sarcomeric); Mib-CK; Basic-Type Mitochondrial Creatine Kinase

  • AA Sequence

    DAREQHKLFPPSADYPDLRKHNNCMAECLTPTIYAKLRNKMTPSGYTLDQCIQTGVDNPGHPFIKTVGMVAGDEESYEVFADLFDPVIKLRHNGYDPRVMKHPTDLDASKITHGQFDERYVLSSRVRTGRSIRGLSLPPACSRAERREVENVAITALEGLKGDLAGRYYKLSEMTEQDQQRLIDDHFLFDKPVSPLLTCAGMARDWPDARGIWHNYDKTFLIWINEEDHTRVISMEKGGNMKRVFERFCRGLKEVERLIQERGWEFMWNERLGYILTCPSNLGTGLRAGVHVRIPKLSKDPRFSKILENLRLQKRGTGGVDTAAVADVYDISNIDRIGRSEVELVQIVIDGVNYLVDCEKKLERGQDIKVPPPLPQFGRK

  • Predicted Molecular Mass

    43.4 kDa

  • Molecular Weight

    Approximately 47 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 750 mM NaCl, pH 6.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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