CMBL Protein, Human (His)
Based on 1 Customer Validation
The CMBL protein is a cysteine hydrolase that converts olmesartan medoxomil to its active form olmesartan, an angiotensin receptor blocker. It may also activate beta-lactam antibiotics such as faropenem etexil and renamcillin. CMBL Protein, Human (His) is the recombinant human-derived CMBL protein, expressed by E. coli , with N-His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The CMBL protein is a cysteine hydrolase that converts olmesartan medoxomil to its active form olmesartan, an angiotensin receptor blocker. It may also activate beta-lactam antibiotics such as faropenem etexil and renamcillin. CMBL Protein, Human (His) is the recombinant human-derived CMBL protein, expressed by E. coli , with N-His labeled tag.
The CMBL protein functions as a cysteine hydrolase, demonstrating the capability to convert the prodrug olmesartan medoxomil into its active metabolite olmerstatan, an angiotensin receptor blocker, primarily within the liver and intestine. Additionally, CMBL may play a role in the activation of beta-lactam antibiotics, such as faropenem medoxomil and lenampicillin. Its enzymatic activity is strongly inhibited by p-chloromercuribenzoate (PCMB) and partially inhibited by bis-p-nitrophenylphosphate (BNPP). Notably, CMBL remains unaffected by inhibitors such as DFP, PMSF, eserine, or EDTA.
1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Measured by its ability to inhibit the proliferation of human colon cancer cells. The ED50 for this effect is 38.07 ng/mL. Corresponding to a specific activity is 2.627×104 Unit/mg
Technical Parameters
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Species Human
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Source E. coli
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Tag N-His
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Accession
Q96DG6 (M1-M245)
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Molecular Construction
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N-term
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His
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CMBL (M1-M245)
Accession # Q96DG6 -
C-term
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Protein Length
Full Length
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Synonyms
CMBL; Carboxymethylenebutenolidase-Like (Pseudomonas); Carboxymethylenebutenolidase Homolog; FLJ23617; Carboxymethylenebutenolidase Homolog (Pseudomonas); JS-1
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AA Sequence
MANEAYPCPCDIGHRLEYGGLGREVQVEHIKAYVTKSPVDAGKAVIVIQDIFGWQLPNTRYIADMISGNGYTTIVPDFFVGQEPWDPSGDWSIFPEWLKTRNAQKIDREISAILKYLKQQCHAQKIGIVGFCWGGTAVHHLMMKYSEFRAGVSVYGIVKDSEDIYNLKNPTLFIFAENDVVIPLKDVSLLTQKLKEHCKVEYQIKTFSGQTHGFVHRKREDCSPADKPYIDEARRNLIEWLNKYM
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Molecular Weight
Approximately 28 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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