CMBL Protein, Human (His)
Based on 1 Customer Validation
The CMBL protein is a cysteine hydrolase that converts olmesartan medoxomil to its active form olmesartan, an angiotensin receptor blocker. It may also activate beta-lactam antibiotics such as faropenem etexil and renamcillin. CMBL Protein, Human (His) is the recombinant human-derived CMBL protein, expressed by E. coli , with N-His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The CMBL protein is a cysteine hydrolase that converts olmesartan medoxomil to its active form olmesartan, an angiotensin receptor blocker. It may also activate beta-lactam antibiotics such as faropenem etexil and renamcillin. CMBL Protein, Human (His) is the recombinant human-derived CMBL protein, expressed by E. coli , with N-His labeled tag.
Background
The CMBL protein functions as a cysteine hydrolase, demonstrating the capability to convert the prodrug olmesartan medoxomil into its active metabolite olmerstatan, an angiotensin receptor blocker, primarily within the liver and intestine. Additionally, CMBL may play a role in the activation of beta-lactam antibiotics, such as faropenem medoxomil and lenampicillin. Its enzymatic activity is strongly inhibited by p-chloromercuribenzoate (PCMB) and partially inhibited by bis-p-nitrophenylphosphate (BNPP). Notably, CMBL remains unaffected by inhibitors such as DFP, PMSF, eserine, or EDTA.
Verified Bioactivity
1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Measured by its ability to inhibit the proliferation of human colon cancer cells. The ED50 for this effect is 38.07 ng/mL. Corresponding to a specific activity is 2.627×104 Unit/mg
Technical Parameters
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Species Human
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Source E. coli
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Tag N-His
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Accession
Q96DG6 (M1-M245)
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Molecular Construction
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N-term
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His
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CMBL (M1-M245)
Accession # Q96DG6 -
C-term
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Protein Length
Full Length
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Synonyms
CMBL; Carboxymethylenebutenolidase-Like (Pseudomonas); Carboxymethylenebutenolidase Homolog; FLJ23617; Carboxymethylenebutenolidase Homolog (Pseudomonas); JS-1
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AA Sequence
MANEAYPCPCDIGHRLEYGGLGREVQVEHIKAYVTKSPVDAGKAVIVIQDIFGWQLPNTRYIADMISGNGYTTIVPDFFVGQEPWDPSGDWSIFPEWLKTRNAQKIDREISAILKYLKQQCHAQKIGIVGFCWGGTAVHHLMMKYSEFRAGVSVYGIVKDSEDIYNLKNPTLFIFAENDVVIPLKDVSLLTQKLKEHCKVEYQIKTFSGQTHGFVHRKREDCSPADKPYIDEARRNLIEWLNKYM
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Molecular Weight
Approximately 28 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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