CUL5 Protein, Human (sf9)
Based on 1 Customer Validation
The CUL5 protein is a core component of the SCF-like ECS E3 ubiquitin-protein ligase complex. CUL5 Protein, Human (sf9) is the recombinant human-derived CUL5 protein, expressed by sf9 insect cells , with tag free.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The CUL5 protein is a core component of the SCF-like ECS E3 ubiquitin-protein ligase complex. CUL5 Protein, Human (sf9) is the recombinant human-derived CUL5 protein, expressed by sf9 insect cells , with tag free.
CUL5 protein serves as a core component in multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, orchestrating the ubiquitination and subsequent proteasomal degradation of target proteins. Functioning as a scaffold protein, CUL5 likely contributes to catalysis by precisely positioning the substrate and the ubiquitin-conjugating enzyme. The functional specificity of these E3 ligase complexes relies on the distinct substrate recognition components. ECS(SOCS1) is implicated in directing the ubiquitination of JAK2, while the ECS(KLHDC1) complex is part of the DesCEND pathway, facilitating the ubiquitination and degradation of truncated SELENOS selenoprotein resulting from failed UGA/Sec decoding. Additionally, CUL5, as part of a multisubunit complex, polyubiquitinates monoubiquitinated POLR2A and may form a cell surface vasopressin receptor. In the context of microbial infection, CUL5 appears to play a role in the proteasomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag Tag Free
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Accession
Q93034 (A2-A780)
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Gene ID/
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Molecular Construction
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N-term
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CUL5 (A2-A780)
Accession # Q93034 -
C-term
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Protein Length
Partial
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Synonyms
CUL5; CUL-5; Cullin 5; VACM1; VACM-1; Cullin-5 (Vasopressin-Activated Calcium-Mobilizing Receptor-1); Cullin-5; Vasopressin-Activated Calcium-Mobilizing Receptor-1; Vasopressin-Activated Calcium-Mobilizing Receptor 1
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AA Sequence
ATSNLLKNKGSLQFEDKWDFMRPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGPAKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYEPQVNSPKDFTEGTLFSVNQEFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYIRRDESDINTFIYMA
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Molecular Weight
Approximately 91 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (240 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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