CYP21A2 Protein, Human (His)

CYP21A2 Protein, a cytochrome P450 monooxygenase, crucially hydroxylates progesterone and 17alpha-hydroxyprogesterone at C-21, yielding vital intermediates for mineralocorticoid and glucocorticoid biosynthesis in adrenal steroidogenesis. This enzymatic process involves molecular oxygen, with NADPH providing essential electrons via cytochrome P450 reductase (CPR). CYP21A2 plays a pivotal role in regulating adrenal cortex hormone production. CYP21A2 Protein, Human (His) is the recombinant human-derived CYP21A2 protein, expressed by E. coli, with N-6*His labeled tag. The total length of CYP21A2 Protein, Human (His) is 494 a.a., with molecular weight of ~59.9 kDa.

The CYP21A2 protein, a cytochrome P450 monooxygenase, plays a pivotal role in adrenal steroidogenesis by catalyzing the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone. This enzymatic activity leads to the formation of crucial intermediate metabolites, namely 11-deoxycorticosterone and 11-deoxycortisol, which are integral components in the biosynthetic pathway of mineralocorticoids and glucocorticoids. Mechanistically, CYP21A2 utilizes molecular oxygen, inserting one oxygen atom into the substrate and reducing the second into a water molecule. The necessary electrons for this process are provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). These biochemical transformations orchestrated by CYP21A2 are essential for the synthesis of key adrenal steroids, contributing significantly to the regulation of mineralocorticoid and glucocorticoid production in the adrenal cortex.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

P08686 (M1-Q494)

1589  [NCBI]

MLLLGLLLLPLLAGARLLWNWWKLRSLHLPPLAPGFLHLLQPDLPIYLLGLTQKFGPIYRLHLGLQDVVVLNSKRTIEEAMVKKWADFAGRPEPLTYKLVSKNYPDLSLGDYSLLWKAHKKLTRSALLLGIRDSMEPVVEQLTQEFCERMRAQPGTPVAIEEEFSLLTCSIICYLTFGDKIKDDNLMPAYYKCIQEVLKTWSHWSIQIVDVIPFLRFFPNPGLRRLKQAIEKRDHIVEMQLRQHKESLVAGQWRDMMDYMLQGVAQPSMEEGSGQLLEGHVHMAAVDLLIGGTETTANTLSWAVVFLLHHPEIQQRLQEELDHELGPGASSSRVPYKDRARLPLLNATIAEVLRLRPVVPLALPHRTTRPSSISGYDIPEGTVIIPNLQGAHLDETVWERPHEFWPDRFLEPGKNSRALAFGCGARVCLGEPLARLELFVVLTRLLQAFTLLPSGDALPSLQPLPHCSVILKMQPFQVRLQPRGMGAHSPGQNQ

Approximately 59.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm solution of Tris-based buffer, 50% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.