CYP7A1 Protein, Human (His)
Based on 1 Customer Validation
CYP7A1 Protein, Human (His) is the recombinant human-derived CYP7A1, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
CYP7A1 Protein, Human (His) is the recombinant human-derived CYP7A1, expressed by E. coli , with N-6*His labeled tag.
Background
CYP7A1 is a cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). It utilizes molecular oxygen to insert one oxygen atom into a substrate while reducing the second into a water molecule, with two electrons supplied by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). As a critical regulatory enzyme in bile acid biosynthesis and cholesterol homeostasis, CYP7A1 catalyzes the hydroxylation of the 7-alpha carbon-hydrogen bond of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid synthesis. Additionally, it 7-alpha hydroxylates various oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol, and oxidizes the 7,8 double bond of 7-dehydrocholesterol and lathosterol, predominantly forming 7-keto derivatives.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
P22680 (M1-L504)
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Gene ID1581
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Protein Length
Full Length
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Synonyms
CYP7A1; CYPVII; Prev. CYP7; Cytochrome P450, Subfamily VIIA Polypeptide 1; Cytochrome P450, Family 7, Subfamily A, Polypeptide 1; Cholesterol 7 Alpha-Monooxygenase; 24-Hydroxycholesterol 7-Alpha-Hydroxylase; Cholesterol 7alpha-Hydroxylase; Cholesterol 7-A
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AA Sequence
MMTTSLIWGIAIAACCCLWLILGIRRRQTGEPPLENGLIPYLGCALQFGANPLEFLRANQRKHGHVFTCKLMGKYVHFITNPLSYHKVLCHGKYFDWKKFHFATSAKAFGHRSIDPMDGNTTENINDTFIKTLQGHALNSLTESMMENLQRIMRPPVSSNSKTAAWVTEGMYSFCYRVMFEAGYLTIFGRDLTRRDTQKAHILNNLDNFKQFDKVFPALVAGLPIHMFRTAHNAREKLAESLRHENLQKRESISELISLRMFLNDTLSTFDDLEKAKTHLVVLWASQANTIPATFWSLFQMIRNPEAMKAATEEVKRTLENAGQKVSLEGNPICLSQAELNDLPVLDSIIKESLRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDIIALYPQLMHLDPEIYPDPLTFKYDRYLDENGKTKTTFYCNGLKLKYYYMPFGSGATICPGRLFAIHEIKQFLILMLSYFELELIEGQAKCPPLDQSRAGLGILPPLNDIEFKYKFKHL
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Predicted Molecular Mass
61.7 kDa
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Molecular Weight
Approximately 64 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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