2. Recombinant Proteins
  3. Ubiquitin Related Proteins
  4. DCAF16 Protein, Human (Sf9, His)

DCAF16 Protein, Human (Sf9, His)

Cat. No.: HY-P701518
Handling Instructions

DCAF16 Protein, a vital component in the CUL4-DDB1 E3 ubiquitin-protein ligase complex, serves as a crucial substrate recognition element. It plays a central role in ubiquitination and subsequent proteasome-dependent degradation of nuclear proteins, emphasizing its key involvement in protein modification processes, particularly in the regulation of protein ubiquitination. DCAF16 Protein, Human (Sf9, His) is the recombinant human-derived DCAF16 protein, expressed by Sf9 insect cells , with N-6*His labeled tag. The total length of DCAF16 Protein, Human (Sf9, His) is 215 a.a., .

For research use only. We do not sell to patients.

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Description

DCAF16 Protein, a vital component in the CUL4-DDB1 E3 ubiquitin-protein ligase complex, serves as a crucial substrate recognition element. It plays a central role in ubiquitination and subsequent proteasome-dependent degradation of nuclear proteins, emphasizing its key involvement in protein modification processes, particularly in the regulation of protein ubiquitination. DCAF16 Protein, Human (Sf9, His) is the recombinant human-derived DCAF16 protein, expressed by Sf9 insect cells , with N-6*His labeled tag. The total length of DCAF16 Protein, Human (Sf9, His) is 215 a.a., .
Background

DCAF16 protein serves as a crucial substrate recognition component within the CUL4-DDB1 E3 ubiquitin-protein ligase complex, playing a central role in the ubiquitination and subsequent proteasome-dependent degradation of nuclear proteins. This function highlights its involvement in protein modification processes, specifically in the regulation of protein ubiquitination.
Species

Human
Source

Sf9 insect cells
Tag

N-6*His
Accession

Q9NXF7 (G2-L216)
Gene ID

54876
Molecular Construction
N-term
6*His
DCAF16 (G2-L216)
Accession # Q9NXF7
C-term
Synonyms
DCAF16; DDB1- and CUL4-associated factor 16
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

DCAF16 Protein, Human (Sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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