DCAF16-DDB1 Protein, Human (sf9, His)
Based on 1 Customer Validation
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Verified Bioactivity
The ubiquitin conjugating activity of His-DCAF16/DDB1 was validated through its ability to catalyse the generation of polyubiquitin chains in the presence of the E1 activating enzyme UBE1, the E2 conjugating enzyme Ubch5b and Bodipy-ubiquitin. Incubation of His-DCAF16/DDB1 for 60 minutes at 37˚C in the presence of Bodipy-ubiquitin, UBE1, Ubch5b and ATP was compared alongside two control reactions with either HisDCAF16/DDB1 or His-DCAF16/DDB1&ATP excluded from the reaction. Ubiquitin conjugates were identified by the migration of the Bodipy-ubiquitin band and these were observed only in the presence of ATP and His-DCAF16/DDB1.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-6*His
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Accession
Q9NXF7 (G2-L216)&Q16531-1 (M1-H1140)
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Gene ID54876&1642
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Molecular Construction
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N-term
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6*His
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DCAF16 (G2-L216)
Accession # Q9NXF7 -
C-term
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N-term
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DDB1 (M1-H1140)
Accession # Q16531-1 -
C-term
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Protein Length
Full Length
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Synonyms
DCAF16; DDB1- And CUL4-Associated Factor 16; Prev. C4orf30; Chromosome 4 Open Reading Frame 30; DDB1 And CUL4 Associated Factor 16; FLJ20280; DDB1; XPE-BF; Damage Specific DNA Binding Protein 1; XAP-1; DNA Damage-Binding Protein 1; XAP1; Xeroderma Pigment
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Predicted Molecular Mass
25.9 kDa & 126.9 kDa
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Molecular Weight
Approximately 26 kDa & 126 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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