DCTN1 Protein, Human (His-SUMO)
The DCTN1 protein is a central player in intracellular trafficking within the dynein complex, guiding dynein-driven retrograde movement along microtubules. It recruits dynein to microtubules, enhancing vesicle and organelle transport. DCTN1 Protein, Human (His-SUMO) is the recombinant human-derived DCTN1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The DCTN1 protein is a central player in intracellular trafficking within the dynein complex, guiding dynein-driven retrograde movement along microtubules. It recruits dynein to microtubules, enhancing vesicle and organelle transport. DCTN1 Protein, Human (His-SUMO) is the recombinant human-derived DCTN1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
Background
DCTN1 protein plays a pivotal role in intracellular transport as a key component of the dynactin complex, orchestrating dynein-mediated retrograde transport along microtubules. It acts by recruiting and tethering dynein to microtubules, facilitating the efficient movement of vesicles and organelles. Beyond its role in transport, DCTN1 regulates microtubule stability by promoting polymerization and inhibiting catastrophe, impacting processes such as spindle orientation, centriole cohesion, and primary cilia formation. Additionally, DCTN1 serves as a molecular brake to modulate the speed of dynein motors during microtubule motility. Its interactions with various proteins, including MAPRE family members, dynein components, and regulatory factors like PLK1 and BCCIP, underscore its multifaceted involvement in cellular processes crucial for proper organelle dynamics and microtubule organization.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-His;N-SUMO
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Accession
Q14203 (P213-Q547)
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Molecular Construction
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N-term
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6*His-SUMO
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DCTN1 (P213-Q547)
Accession # Q14203 -
C-term
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Protein Length
Partial
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Synonyms
DCTN1; Dynactin 1 (P150, Glued (Drosophila) Homolog); Dynactin Subunit 1; Dynactin 1 (P150, Glued Homolog, Drosophila); 150 KDa Dynein-Associated Polypeptide; P150 Glued Homolog (Drosophila); DAP-150; Alternative Protein DCTN1; P150-Glued; DKFZp686E0752;
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AA Sequence
PSKEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQ
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Predicted Molecular Mass
55.2 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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