DCTN1 Protein, Human (His-SUMO)

The DCTN1 protein is a central player in intracellular trafficking within the dynein complex, guiding dynein-driven retrograde movement along microtubules. It recruits dynein to microtubules, enhancing vesicle and organelle transport. DCTN1 Protein, Human (His-SUMO) is the recombinant human-derived DCTN1 protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of DCTN1 Protein, Human (His-SUMO) is 335 a.a., with molecular weight of ~55.2 kDa.

DCTN1 protein plays a pivotal role in intracellular transport as a key component of the dynactin complex, orchestrating dynein-mediated retrograde transport along microtubules. It acts by recruiting and tethering dynein to microtubules, facilitating the efficient movement of vesicles and organelles. Beyond its role in transport, DCTN1 regulates microtubule stability by promoting polymerization and inhibiting catastrophe, impacting processes such as spindle orientation, centriole cohesion, and primary cilia formation. Additionally, DCTN1 serves as a molecular brake to modulate the speed of dynein motors during microtubule motility. Its interactions with various proteins, including MAPRE family members, dynein components, and regulatory factors like PLK1 and BCCIP, underscore its multifaceted involvement in cellular processes crucial for proper organelle dynamics and microtubule organization.

Human

E. coli

N-His;N-SUMO

Q14203 (213P-547Q)

1639  [NCBI]

PSKEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQ

Approximately 55.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.