DGCR8 Protein, Human (His, Strep)
DGCR8 Protein, Human (His, Strep) is the recombinant human-derived DGCR8, expressed by E. coli , with Strep, His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
DGCR8 Protein, Human (His, Strep) is the recombinant human-derived DGCR8, expressed by E. coli , with Strep, His labeled tag.
Background
DGCR8 is a component of the microprocessor complex that functions as an RNA- and heme-binding protein involved in the initial step of microRNA (miRNA) biogenesis. Within the microprocessor complex, DGCR8 processes primary miRNA transcripts (pri-miRNAs) into precursor miRNAs (pre-miRNAs). It acts as a molecular anchor to recognize the dsRNA-ssRNA junction of pri-miRNAs and directs DROSHA to cleave 11 bp away from the junction, releasing hairpin-shaped pre-miRNAs for subsequent processing by cytoplasmic DICER into mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs cooperatively as a trimer (of dimers) to trigger cleavage, while the heme-free monomer binds as a dimer with reduced activity. Both double-stranded and single-stranded regions of pri-miRNAs are required for binding. DGCR8 specifically recognizes and binds N6-methyladenosine (m6A)-modified pri-miRNAs, a key modification for processing. It is also involved in silencing embryonic stem cell self-renewal (by similar) and plays a role in DNA repair by promoting RNF168 recruitment to RNF8 and MDC1 at double-strand breaks for DNA damage clearance.
Technical Parameters
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Species Human
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Source E. coli
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Tag Strep;His
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Accession
Q8WYQ5-1 (M1-V773)
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Gene ID54487
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Protein Length
Full Length of Isoform-1
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Synonyms
DGCR8; Gy1; Prev. C22orf12; DiGeorge Syndrome Critical Region 8; DGCRK6; DGCR8, Microprocessor Complex Subunit; Microprocessor Complex Subunit DGCR8; Chromosome 22 Open Reading Frame 12; Pasha; Alternative Protein DGCR8; DGCR8 Microprocessor Complex Subun
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AA Sequence
METDESPSPLPCGPAGEAVMESRARPFQALPREQSPPPPLQTSSGAEVMDVGSGGDGQSELPAEDPFNFYGASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAVRKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGAERDVRAECGLLLSPVSGDVHACPFGGSVGDGVGIGGESADKKDEENELDQEKRVEYAVLDELEDFTDNLELDEEGAGGFTAKAIVQRDRVDEEALNFPYEDDFDNDVDALLEEGLCAPKKRRTEEKYGGDSDHPSDGETSVQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSGVPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSIPCLHYKKMKDNEEREQSSDLTPSGDVSPVKPLSRSAELEFPLDEPDSMGADPGPPDEKDPLGAEAAPGALGQVKAKVEVCKDESVDLEEFRSYLEKRFDFEQVTVKKFRTWAERRQFNREMKRKQAESERPILPANQKLITLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLKVRPVYNFFECENPSEPFGASVTIDGVTYGSGTASSKKLAKNKAARATLEILIPDFVKQTSEEKPKDSEELEYFNHISIEDSRVYELTSKAGLLSPYQILHECLKRNHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGWCKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNKPNLHILSKLQEEMKRLAEEREETRKKPKMSIVASAQPGGEPLCTVDV
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Predicted Molecular Mass
89.2 kDa
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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