Dynamitin (DCTN2) Protein, Human (HEK293, His)
Based on 1 Customer Validation
Dynamitin (DCTN2) Protein, Human (HEK293, His) is the recombinant human-derived Dynamitin (DCTN2), expressed by HEK293 , with C-10*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Dynamitin (DCTN2) Protein, Human (HEK293, His) is the recombinant human-derived Dynamitin (DCTN2), expressed by HEK293 , with C-10*His labeled tag.
Background
Dynamitin (DCTN2) is a component of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules. Within the dynactin shoulder domain, it binds the ACTR1A filament and functions as a molecular ruler to determine filament length (By similarity). It modulates cytoplasmic dynein binding to organelles and contributes to prometaphase chromosome alignment and spindle organization during mitosis. Additionally, it is involved in anchoring microtubules to centrosomes and may play a role in synapse formation during brain development (By similarity).
Technical Parameters
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Species Human
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Source HEK293
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Tag C-10*His
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Accession
NP_006391 (M1-K406)
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Gene ID10540
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Protein Length
Full Length
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Synonyms
DCTN2; P50 Dynamitin; Dynactin Subunit 2; DCTN50; DCTN-50; Epididymis Secretory Sperm Binding Protein; RBP50; Epididymis Secretory Protein Li 77; 50 KDa Dynein-Associated Polypeptide; DYNAMITIN; Dynactin Complex 50 KDa Subunit; HEL-S-77; Dynactin 2 (P50)
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AA Sequence
MADPKYADLPGIARNEPDVYETSDLPEDDQAEFDAFAQELEELTSTSVEHIIVNPNAAYDKFKDKRVGTKGLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK
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Predicted Molecular Mass
44.8 kDa
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Molecular Weight
Approximately 56-70 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 25 mM Tris-HCl, 100 mM Glycine, pH 7.3, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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