Dynamitin (DCTN2) Protein, Human (HEK293, His)

Dynamitin (DCTN2) Protein, Human (HEK293, His) is the recombinant human-derived Dynamitin (DCTN2), expressed by HEK293 , with C-10*His labeled tag.

Dynamitin (DCTN2) is a component of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules. Within the dynactin shoulder domain, it binds the ACTR1A filament and functions as a molecular ruler to determine filament length (By similarity). It modulates cytoplasmic dynein binding to organelles and contributes to prometaphase chromosome alignment and spindle organization during mitosis. Additionally, it is involved in anchoring microtubules to centrosomes and may play a role in synapse formation during brain development (By similarity).

Human

HEK293

C-10*His

NP_006391 (M1-K406)

10540

Full Length

MADPKYADLPGIARNEPDVYETSDLPEDDQAEFDAFAQELEELTSTSVEHIIVNPNAAYDKFKDKRVGTKGLDFSDRIGKTKRTGYESGEYEMLGEGLGVKETPQQKYQRLLHEVQELTTEVEKIKTTVKESATEEKLTPVLLAKQLAALKQQLVASHLEKLLGPDAAINLTDPDGALAKRLLLQLEATKNSKGGSGGKTTGTPPDSSLVTYELHSRPEQDKFSQAAKVAELEKRLTELETAVRCDQDAQNPLSAGLQGACLMETVELLQAKVSALDLAVLDQVEARLQSVLGKVNEIAKHKASVEDADTQSKVHQLYETIQRWSPIASTLPELVQRLVTIKQLHEQAMQFGQLLTHLDTTQQMIANSLKDNTTLLTQVQTTMRENLATVEGNFASIDERMKKLGK

Approximately 56-70 kDa due to the glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 25 mM Tris-HCl, 100 mM Glycine, pH 7.3, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.