Elastase IIIB/CELA3B Protein, Mouse (Myc, His)
The elastase IIIB/CELA3B protein exhibits remarkable efficiency as a protease, showing a clear preference for alanine specificity while exhibiting limited elastolytic activity. This unique zymogram makes this protein a promising candidate for precise substrate cleavage, especially when processing alanine-rich substrates. Elastase IIIB/CELA3B Protein, Mouse (Myc, His) is the recombinant mouse-derived Elastase IIIB/CELA3B protein, expressed by E. coli , with N-His, C-Myc labeled tag.
- Species: Mouse
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The elastase IIIB/CELA3B protein exhibits remarkable efficiency as a protease, showing a clear preference for alanine specificity while exhibiting limited elastolytic activity. This unique zymogram makes this protein a promising candidate for precise substrate cleavage, especially when processing alanine-rich substrates. Elastase IIIB/CELA3B Protein, Mouse (Myc, His) is the recombinant mouse-derived Elastase IIIB/CELA3B protein, expressed by E. coli , with N-His, C-Myc labeled tag.
Background
Elastase IIIB, also known as CELA3B, is an efficient protease with a notable specificity for alanine but exhibits only limited elastolytic activity. This enzyme is characterized by its ability to cleave peptide bonds specifically at alanine residues, indicating a preference for substrates containing this amino acid. While it does not demonstrate strong elastolytic activity, its efficient proteolytic function suggests a role in the targeted cleavage of proteins containing alanine residues. The unique substrate specificity of Elastase IIIB/CELA3B underscores its distinctive role in proteolytic processes within biological systems.
Technical Parameters
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Species Mouse
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Source E. coli
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Tag N-His;C-Myc
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Accession
Q9CQ52 (V28-N269)
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Molecular Construction
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N-term
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10*His
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CELA3B (V28-N269)
Accession # Q9CQ52 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
CELA3B; Proteinase E; Prev. ELA3B; Protease E; CBPP; Chymotrypsin-Like Elastase Family, Member 3B; Chymotrypsin-Like Elastase Family Member 3B; Chymotrypsin Like Elastase Family Member 3B; Cholesterol-Binding Pancreatic Protease; Pancreatic Elastase 1; Pa
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AA Sequence
VVNGEEAVPHSWPWQVSLQYEKDGSFHHTCGGSLITPDWVLTAGHCISTSRTYQVVLGEHERGVEEGQEQVIPINAGDLFVHPKWNSMCVSCGNDIALVKLSRSAQLGDAVQLACLPPAGEILPNGAPCYISGWGRLSTNGPLPDKLQQALLPVVDYEHCSRWNWWGLSVKTTMVCAGGDIQSGCNGDSGGPLNCPADNGTWQVHGVTSFVSSLGCNTLRKPTVFTRVSAFIDWIEETIANN
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Predicted Molecular Mass
31.1 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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