ELAVL1 Protein, Human (Flag)
Based on 1 Customer Validation
ELAVL1 Protein, Human (Flag) is the recombinant human-derived ELAVL1, expressed by E. coli , with C-Flag labeled tag.
- Species: Human
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ELAVL1 Protein, Human (Flag) is the recombinant human-derived ELAVL1, expressed by E. coli , with C-Flag labeled tag.
Background
HuR is an RNA-binding protein that binds to the 3'-UTR of mRNAs to enhance their stability. It plays a role in embryonic stem cell (ESC) differentiation by preferentially stabilizing non-m6A-methylated mRNAs, thereby promoting ESC differentiation (By similarity). It can also bind m6A-containing mRNAs, such as MYC mRNA, contributing to MYC stability. HuR targets poly-U and AU-rich elements (AREs) in the 3'-UTR of mRNAs, including those in FOS and IL3/interleukin-3 mRNAs. For FOS, it binds a 27-nucleotide core ARE containing AUUUA, AUUUUA, and AUUUUUA motifs, with a preference for the 5'-UUUU[AG]UUU-3' motif in vitro. In collaboration with ZNF385A, HuR binds the 3'-UTR of p53/TP53 mRNA to regulate its CDKN2A-induced nuclear export, potentially modulating p53/TP53 expression and partially mediating CDKN2A's anti-proliferative effects (By similarity). Additionally, it stabilizes leptin mRNA containing an ARE in its 3'-UTR.
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag C-Flag
-
Accession
Q15717-1 (S2-K326)
-
Gene ID1994
-
Protein Length
Full Length of Isoform-1
-
Synonyms
ELAVL1; Embryonic Lethal, Abnormal Vision, Drosophila, Homolog-Like 1; Prev. HUR; Human Antigen R; HuR; Hu Antigen R; MelG; Hu-Antigen R; Hua; ELAV1; ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R); ELAV Like RNA Binding Protein
-
AA Sequence
SNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK
-
Predicted Molecular Mass
37.1 kDa
-
Molecular Weight
Approximately 35 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
2.Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (237 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
