EPHX1 Protein, Human (His-SUMO)

The EPHX1 protein is an important biotransformation enzyme that hydrolyzes aromatic hydrocarbons and aliphatic epoxides by catalyzing the transaddition of water. This makes the epoxide less reactive and more water-soluble. EPHX1 Protein, Human (His-SUMO) is the recombinant human-derived EPHX1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of EPHX1 Protein, Human (His-SUMO) is 455 a.a., with molecular weight of ~66 kDa.

The EPHX1 protein serves as a crucial biotransformation enzyme, facilitating the hydrolysis of both arene and aliphatic epoxides. Through the trans addition of water, EPHX1 catalyzes this transformation, rendering the epoxides less reactive and more water-soluble. Beyond its role in xenobiotic metabolism, EPHX1 plays a significant role in the metabolic pathways of endogenous lipids, particularly in the processing of epoxide-containing fatty acids. Notably, EPHX1 participates in the metabolism of the abundant endocannabinoid 2-arachidonoylglycerol (2-AG), converting it into free arachidonic acid (AA) and glycerol. These enzymatic activities highlight the versatile role of EPHX1 in modulating the bioavailability and reactivity of various substrates, contributing to essential cellular processes and lipid metabolism.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His;N-SUMO

P07099 (M1-Q455)

2052  [NCBI]

MWLEILLTSVLGFAIYWFISRDKEETLPLEDGWWGPGTRSAAREDDSIRPFKVETSDEEIHDLHQRIDKFRFTPPLEDSCFHYGFNSNYLKKVISYWRNEFDWKKQVEILNRYPHFKTKIEGLDIHFIHVKPPQLPAGHTPKPLLMVHGWPGSFYEFYKIIPLLTDPKNHGLSDEHVFEVICPSIPGYGFSEASSKKGFNSVATARIFYKLMLRLGFQEFYIQGGDWGSLICTNMAQLVPSHVKGLHLNMALVLSNFSTLTLLLGQRFGRFLGLTERDVELLYPVKEKVFYSLMRESGYMHIQCTKPDTVGSALNDSPVGLAAYILEKFSTWTNTEFRYLEDGGLERKFSLDDLLTNVMLYWTTGTIISSQRFYKENLGQGWMTQKHERMKVYVPTGFSAFPFELLHTPEKWVRFKYPKLISYSYMVRGGHFAAFEEPELLAQDIRKFLSVLERQ

Approximately 66 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.