EPHX1 Protein, Human (His-SUMO)
Based on 1 Customer Validation
The EPHX1 protein is an important biotransformation enzyme that hydrolyzes aromatic hydrocarbons and aliphatic epoxides by catalyzing the transaddition of water. This makes the epoxide less reactive and more water-soluble. EPHX1 Protein, Human (His-SUMO) is the recombinant human-derived EPHX1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.
- Species: Human
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The EPHX1 protein is an important biotransformation enzyme that hydrolyzes aromatic hydrocarbons and aliphatic epoxides by catalyzing the transaddition of water. This makes the epoxide less reactive and more water-soluble. EPHX1 Protein, Human (His-SUMO) is the recombinant human-derived EPHX1 protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag.
Background
The EPHX1 protein serves as a crucial biotransformation enzyme, facilitating the hydrolysis of both arene and aliphatic epoxides. Through the trans addition of water, EPHX1 catalyzes this transformation, rendering the epoxides less reactive and more water-soluble. Beyond its role in xenobiotic metabolism, EPHX1 plays a significant role in the metabolic pathways of endogenous lipids, particularly in the processing of epoxide-containing fatty acids. Notably, EPHX1 participates in the metabolism of the abundant endocannabinoid 2-arachidonoylglycerol (2-AG), converting it into free arachidonic acid (AA) and glycerol. These enzymatic activities highlight the versatile role of EPHX1 in modulating the bioavailability and reactivity of various substrates, contributing to essential cellular processes and lipid metabolism.
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag N-6*His;N-SUMO
-
Accession
P07099 (M1-Q455)
-
Molecular Construction
-
N-term
-
6*His-SUMO
-
EPHX1 (M1-Q455)
Accession # P07099 -
C-term
-
-
Protein Length
Full Length
-
Synonyms
EPHX1; EPOX; Prev. EPHX; Epoxide Hydrolase 1 Microsomal Isoform 2; Epoxide Hydratase; Epoxide Hydrolase 1 Microsomal; Epoxide Hydrolase 1, Microsomal (Xenobiotic); Epoxide Hydrolase; Microsomal Epoxide Hydrolase; HYL1; Epoxide Hydrolase 1; MEH
-
AA Sequence
MWLEILLTSVLGFAIYWFISRDKEETLPLEDGWWGPGTRSAAREDDSIRPFKVETSDEEIHDLHQRIDKFRFTPPLEDSCFHYGFNSNYLKKVISYWRNEFDWKKQVEILNRYPHFKTKIEGLDIHFIHVKPPQLPAGHTPKPLLMVHGWPGSFYEFYKIIPLLTDPKNHGLSDEHVFEVICPSIPGYGFSEASSKKGFNSVATARIFYKLMLRLGFQEFYIQGGDWGSLICTNMAQLVPSHVKGLHLNMALVLSNFSTLTLLLGQRFGRFLGLTERDVELLYPVKEKVFYSLMRESGYMHIQCTKPDTVGSALNDSPVGLAAYILEKFSTWTNTEFRYLEDGGLERKFSLDDLLTNVMLYWTTGTIISSQRFYKENLGQGWMTQKHERMKVYVPTGFSAFPFELLHTPEKWVRFKYPKLISYSYMVRGGHFAAFEEPELLAQDIRKFLSVLERQ
-
Predicted Molecular Mass
71.1 kDa
-
Molecular Weight
Approximately 66 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
1.Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (238 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
