Exopolyphosphatase/PPX Protein, E.coli (His-SUMO)
Based on 1 Customer Validation
Exopolyphosphatase/PPX proteins are dedicated to inorganic polyphosphate (polyP) degradation, expertly releasing orthophosphate from polyphosphate termini in a sustained manner. It shows a strong preference for long-chain polyphosphates, with moderate affinity for shorter sizes (approximately 15 residues). Exopolyphosphatase/PPX Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Exopolyphosphatase/PPX protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Species: E.coli
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Exopolyphosphatase/PPX proteins are dedicated to inorganic polyphosphate (polyP) degradation, expertly releasing orthophosphate from polyphosphate termini in a sustained manner. It shows a strong preference for long-chain polyphosphates, with moderate affinity for shorter sizes (approximately 15 residues). Exopolyphosphatase/PPX Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Exopolyphosphatase/PPX protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
Background
Exopolyphosphatase/PPX protein, specializing in the degradation of inorganic polyphosphates (polyP), proficiently releases orthophosphate in a processive manner from the termini of the polyP chain. Demonstrating a robust predilection for long-chain polyphosphates, this enzyme exhibits only modest affinity for smaller polyP sizes, typically around 15 residues. With its precision in catalyzing the breakdown of polyP, Exopolyphosphatase/PPX plays a crucial role in regulating phosphate metabolism and contributes to the dynamic balance of cellular phosphate pools.
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species E.coli
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Source E. coli
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Tag N-His;N-SUMO
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Accession
P0AFL6 (P2-A513)
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Gene ID946970 [NCBI]
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Molecular Construction
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N-term
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6*His-SUMO
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Exopolyphosphatase/PPX (P2-A513)
Accession # P0AFL6 -
C-term
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Protein Length
Full Length
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Synonyms
PPP4C; PPP4; Protein Phosphatase 4 Catalytic Subunit; Epididymis Secretory Sperm Binding Protein; PPX; Protein Phosphatase 4, Catalytic Subunit; PP4; Serine/Threonine-Protein Phosphatase; Serine/Threonine-Protein Phosphatase 4 Catalytic Subunit; Protein-S
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AA Sequence
PIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQRVHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFLKRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPILVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASGTIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLAILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQARRVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNSDLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEESTPEIAA
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Predicted Molecular Mass
74 kDa
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Molecular Weight
Approximately 74 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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