Gamma-GCS Protein, E.coli (His-SUMO)
Gamma-GCS protein is a key enzyme in sulfur metabolism and initiates glutathione synthesis by catalyzing the incorporation of L-cysteine and L-glutamic acid. This important molecule critical for cellular antioxidant defense is synthesized through a critical 1/2 step facilitated by γ-GCS. Gamma-GCS Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Gamma-GCS protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Species: E.coli
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Gamma-GCS protein is a key enzyme in sulfur metabolism and initiates glutathione synthesis by catalyzing the incorporation of L-cysteine and L-glutamic acid. This important molecule critical for cellular antioxidant defense is synthesized through a critical 1/2 step facilitated by γ-GCS. Gamma-GCS Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Gamma-GCS protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
Gamma-GCS protein, a key enzyme in sulfur metabolism and glutathione biosynthesis, facilitates the critical step 1/2 of glutathione formation from L-cysteine and L-glutamate. As part of this enzymatic process, gamma-GCS catalyzes the incorporation of L-cysteine and L-glutamate, initiating the synthesis of glutathione. This molecule, essential for cellular antioxidant defense and detoxification, plays a crucial role in maintaining redox homeostasis within the cell. By orchestrating the initial steps of glutathione biosynthesis, gamma-GCS contributes to the cellular mechanisms that protect against oxidative stress and support overall cellular health.
Technical Parameters
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Species E.coli
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Source E. coli
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Tag N-His;N-SUMO
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Accession
P0A6W9 (M1-A518)
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Gene ID944881 [NCBI]
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Molecular Construction
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N-term
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6*His-SUMO
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Gamma-GCS (M1-A518)
Accession # P0A6W9 -
C-term
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Protein Length
Full Length
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Synonyms
Glutamate--cysteine ligase; EC:6.3.2.2; Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase; gshA; gsh-I; b2688; JW2663
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AA Sequence
MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGHPEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPLSMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFWQAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPFEKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAKIGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDINPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLGIGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSARILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTEPFAVWLEKHA
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Predicted Molecular Mass
74.3 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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