GNAI2 Protein, Mouse (His)
GNAI2 Protein, Mouse (His) is the recombinant mouse-derived GNAI2 protein, expressed by E. coli, with N-6*His tag.
- Species: Mouse
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
GNAI2 Protein, Mouse (His) is the recombinant mouse-derived GNAI2 protein, expressed by E. coli, with N-6*His tag.
Background
GNAI2 is a Guanine nucleotide-binding protein (G protein) that acts as a modulator or transducer in various transmembrane signaling systems. As a member of the G(i) protein family, it inhibits adenylate cyclase in response to beta-adrenergic stimuli, participating in hormonal regulation. This protein may also play a role in cell division. by similar: Other G protein family members like GNAS are involved in transmembrane signaling but through distinct regulatory mechanisms.
Technical Parameters
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Species Mouse
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Source E. coli
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Tag N-6*His
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Accession
P08752 (G2-F355)
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Gene ID14678
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Molecular Construction
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N-term
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6*His
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P08752 (G2-F355)
Accession # GNAI2 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
GNAI2; GTP-Binding Regulatory Protein Gi Alpha-2 Chain; Prev. GNAI2B; Heterotrimeric Guanine Nucleotide-Binding Protein 1C; Guanine Nucleotide-Binding Protein G(I) Subunit Alpha-2; Guanine Nucleotide-Binding Regulatory Protein; Adenylate Cyclase-Inhibitin
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AA Sequence
GCTVSAEDKAAAERSKMIDKNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEDGYSEEECRQYRAVVYSNTIQSIMAIVKAMGNLQIDFADPQRADDARQLFALSCAAEEQGMLPEDLSGVIRRLWADHGVQACFGRSREYQLNDSAAYYLNDLERIAQSDYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSAYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKITQSSLTICFPEYTGANKYDEAASYIQSKFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF
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Predicted Molecular Mass
44.4 kDa
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Molecular Weight
Approximately 44 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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