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Grb2 Protein, Human (His)

Cat. No.: HY-P70396
COA Handling Instructions

Grb2, an adapter protein, crucially connects cell surface growth factor receptors to the Ras signaling pathway. Despite no direct binding to phosphorylated EGFR, Grb2 inhibits EGF-induced transactivation of a RAS-responsive element. It acts as a dominant negative relative to GRB2, suppressing proliferative signals and potentially initiating programmed cell death. Grb2 Protein, Human (His) is the recombinant human-derived Grb2 protein, expressed by E. coli , with C-6*His labeled tag. The total length of Grb2 Protein, Human (His) is 217 a.a., with molecular weight of 25-29 kDa.

For research use only. We do not sell to patients.

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Description

Grb2, an adapter protein, crucially connects cell surface growth factor receptors to the Ras signaling pathway. Despite no direct binding to phosphorylated EGFR, Grb2 inhibits EGF-induced transactivation of a RAS-responsive element. It acts as a dominant negative relative to GRB2, suppressing proliferative signals and potentially initiating programmed cell death. Grb2 Protein, Human (His) is the recombinant human-derived Grb2 protein, expressed by E. coli , with C-6*His labeled tag. The total length of Grb2 Protein, Human (His) is 217 a.a., with molecular weight of 25-29 kDa.

Background

Grb2, an adapter protein, plays a pivotal role in establishing a crucial connection between cell surface growth factor receptors and the Ras signaling pathway. Despite its lack of direct binding to phosphorylated epidermal growth factor receptor (EGFR), Grb2 exerts an inhibitory effect on EGF-induced transactivation of a RAS-responsive element. Notably, Grb2 functions as a dominant negative protein relative to GRB2, suppressing proliferative signals and potentially instigating active programmed cell death.

Biological Activity

Immobilized Human GRB2 at 2 μg/mL (100 μL/well) can bind Anti-GRB2 Antibody. The ED50 for this effect is 93.39 ng/mL.

Species

Human

Source

E. coli

Tag

C-6*His

Accession

P62993 (M1-V217)

Gene ID
Molecular Construction
N-term
Grb2 (M1-V217)
Accession # P62993
6*His
C-term
Synonyms
rHuGrowth factor receptor-bound protein 2/GRB2, His; Growth Factor Receptor-Bound Protein 2; Adapter Protein GRB2; Protein Ash; SH2/SH3 Adapter GRB2; GRB2; ASH
AA Sequence

MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV

Molecular Weight

Approximately 25-29 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 6% Sucrose, 4% Mannitol, 50 mM NaCl, 0.05% Tween 80, pH 8.0 or 50 mM Tris-HCL, 150 mM NaCl, pH 8.0, 8% trehalose, 0.02% Tween80.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Grb2 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Grb2 Protein, Human (His)
Cat. No.:
HY-P70396
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