GRPR Protein, Human (Cell-free, Twin-Strep)
Based on 1 Customer Validation
GRPR Protein, Human (Cell-free, Twin-Strep) is the recombinant human-derived GRPR, expressed by E. coli Cell-free , with C-Twin-Strep labeled tag.
- Species: Human
- Source: E. coli Cell-free
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
GRPR Protein, Human (Cell-free, Twin-Strep) is the recombinant human-derived GRPR, expressed by E. coli Cell-free , with C-Twin-Strep labeled tag.
Background
GRPR is a receptor for gastrin-releasing peptide (GRP), signaling via G protein-coupled activation of a phosphatidylinositol-calcium second messenger system to induce Akt phosphorylation. It regulates food intake and mediates nonhistaminergic itch sensation in the spinal cord (without pain perception). By similarity, GRPR acts in the amygdala to inhibit fear-associated memory and disinhibits auditory cortex glutamatergic cells via vasoactive intestinal peptide-expressing cells to enhance auditory fear memory. Additionally, it contributes to sigh induction through signaling in the pre-Bötzinger complex, a respiratory rhythm-generating neuronal cluster.
Technical Parameters
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Species Human
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Source E. coli Cell-free
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Tag C-Twin-Strep
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Accession
P30550 (M1-V384)
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Gene ID2925
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Protein Length
Full Length
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Synonyms
GRPR; BRS2; Gastrin Releasing Peptide Receptor; BB2; Gastrin-Releasing Peptide Receptor; Bombesin Receptor 2; GRP-Preferring Bombesin Receptor; GRP-R; BB2R
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AA Sequence
MALNDCFLLNLEVDHFMHCNISSHSADLPVNDDWSHPGILYVIPAVYGVIILIGLIGNITLIKIFCTVKSMRNVPNLFISSLALGDLLLLITCAPVDASRYLADRWLFGRIGCKLIPFIQLTSVGVSVFTLTALSADRYKAIVRPMDIQASHALMKICLKAAFIWIISMLLAIPEAVFSDLHPFHEESTNQTFISCAPYPHSNELHPKIHSMASFLVFYVIPLSIISVYYYFIAKNLIQSAYNLPVEGNIHVKKQIESRKRLAKTVLVFVGLFAFCWLPNHVIYLYRSYHYSEVDTSMLHFVTSICARLLAFTNSCVNPFALYLLSKSFRKQFNTQLLCCQPGLIIRSHSTGRSTTCMTSLKSTNPSVATFSLINGNICHERYV
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Molecular Weight
Approximately 40 kDa & 80 kDa & 160 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, 0.05% Brij-78, 6% trehalose, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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