GSPT1 Protein, Human (Biotinylated, sf9, Avi)
Based on 1 Customer Validation
GSPT1 Protein, Human (Biotinylated, sf9, Avi) is the recombinant human-derived GSPT1, expressed by sf9 insect cells , with Avi labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
GSPT1 Protein, Human (Biotinylated, sf9, Avi) is the recombinant human-derived GSPT1, expressed by sf9 insect cells , with Avi labeled tag.
Background
eRF3a is the GTPase component of the eRF1-eRF3-GTP ternary complex, which mediates translation termination in response to stop codons UAA, UAG, and UGA. GSPT1/ERF3A delivers ETF1/ERF1 to stop codons: The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site, and GTP hydrolysis by GSPT1/ERF3A induces a conformational change leading to its dissociation, allowing ETF1/ERF1 to fully occupy the A-site. Additionally, eRF3a is part of the transient SURF complex that recruits UPF1 to stalled ribosomes during nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. It is also required for SHFL-mediated translation termination, which inhibits programmed ribosomal frameshifting (-1PRF) in viral and cellular mRNAs. by similar: Its functions are supported by studies on complex binding, conformational dynamics, and roles in NMD and frameshifting regulation.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-Avi
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Accession
P15170-2 (D2-D636)
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Gene ID2935
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Molecular Construction
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N-term
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GSPT1 (D2-D637)
Accession # P15170-2 -
C-term
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Protein Length
Full Length of Isoform-2
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Conjugation
Biotin
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Synonyms
GSPT1; Eukaryotic Peptide Chain Release Factor Subunit 3a; G1 To S Phase Transition 1; G1 To S Phase Transition Protein 1 Homolog; ERF3a; Eukaryotic Release Factor 3a; ETF3A; 551G9.2; GST1; ERF3A; Eukaryotic Peptide Chain Release Factor GTP-Binding Subuni
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AA Sequence
DPGSGGGGGGGGGGGSSSGSSSSDSAPDCWDQADMEAPGPGPCGGGGSLAAAAEAQRENLSAAFSRQLNVNAKPFVPNVHAAEFVPSFLRGPAAPPPPVGGAANNHGAGSGAGGRAAPVESSQEEQSLCEGSNSAVSMELSEPIENGETEMSPEESWEHKEEISEAEPGGGSLGDGRPPEESAHEMMEEEEEIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRLKGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD
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Predicted Molecular Mass
70.8 kDa
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Molecular Weight
Approximately 75 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 1 mM DTT, 200 mM NaCl, 20% glycerol.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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