HNF4A Protein, Human (HEK293, His)
Based on 1 publication(s) in Google Scholar
HNF 4 alpha, a nuclear transcription factor, forms homodimers to bind DNA. It regulates gene expression, including hepatocyte nuclear factor 1 alpha, impacting hepatic genes. Its involvement extends to liver, kidney, and intestinal development. Mutations associate with autosomal dominant non-insulin-dependent diabetes mellitus type I. The gene exhibits biased expression in the duodenum (RPKM 37.7) and colon (RPKM 32.8), among other tissues. HNF4A Protein, Human (HEK293, His) is the recombinant human-derived HNF 4 alpha, expressed by HEK293 , with C-10*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
HNF 4 alpha, a nuclear transcription factor, forms homodimers to bind DNA. It regulates gene expression, including hepatocyte nuclear factor 1 alpha, impacting hepatic genes. Its involvement extends to liver, kidney, and intestinal development. Mutations associate with autosomal dominant non-insulin-dependent diabetes mellitus type I. The gene exhibits biased expression in the duodenum (RPKM 37.7) and colon (RPKM 32.8), among other tissues. HNF4A Protein, Human (HEK293, His) is the recombinant human-derived HNF 4 alpha, expressed by HEK293 , with C-10*His labeled tag.
Background
HNF 4 alpha, the protein product of this gene, functions as a nuclear transcription factor, forming homodimers to bind DNA and exerting control over the expression of various genes, including hepatocyte nuclear factor 1 alpha, a key regulator of hepatic gene expression. Its involvement extends to the development of vital organs such as the liver, kidney, and intestines. Mutations in HNF 4 alpha have been linked to monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I, underscoring its significance in metabolic regulation. Additionally, alternative splicing yields multiple transcript variants, generating diverse isoforms. Notably, HNF 4 alpha displays biased expression, with higher levels observed in the duodenum (RPKM 37.7), colon (RPKM 32.8), and seven other tissues, emphasizing its specialized role in diverse physiological contexts.
Publications (1)
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Journal Impact Factor
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Most Recent
Technical Parameters
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Species Human
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Source HEK293
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Tag C-10*His
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Accession
P41235-1/NP_000448 (M1-I474)
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Gene ID3172
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Molecular Construction
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N-term
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Accession # P41235-1/NP_448(M1-I474) -
10*His
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C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
HNF4A; TCF-14; Prev. TCF14; Hepatocyte Nuclear Factor 4 4 Alpha Variant 4; Prev. MODY1; Hepatocyte Nuclear Factor 4 4 Alpha Variant 1; Prev. MODY; Hepatocyte Nuclear Factor 4 4 Alpha Variant 2; NR2A1; Truncated Hepatocyte Nuclear Factor 1 Alpha; HNF4; HNF
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AA Sequence
MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI
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Predicted Molecular Mass
54.2 kDa
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Molecular Weight
Approximately 80 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 8.0, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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