IRE1 Protein, Human (sf9)
Based on 1 Customer Validation
IRE1 is a key serine/threonine protein kinase and endoribonuclease essential for sensing the unfolded protein response (UPR) in the endoplasmic reticulum. In cellular homeostasis, it remains monomeric by binding to HSPA5/BiP. IRE1 Protein, Human (sf9) is the recombinant human-derived IRE1 protein, expressed by Sf9 insect cells , with tag free.
- Species: Human
- Source: Sf9 insect cells
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
IRE1 is a key serine/threonine protein kinase and endoribonuclease essential for sensing the unfolded protein response (UPR) in the endoplasmic reticulum. In cellular homeostasis, it remains monomeric by binding to HSPA5/BiP. IRE1 Protein, Human (sf9) is the recombinant human-derived IRE1 protein, expressed by Sf9 insect cells , with tag free.
IRE1, a serine/threonine-protein kinase and endoribonuclease, serves as a pivotal sensor in the endoplasmic reticulum unfolded protein response (UPR). In the absence of cellular stress, the endoplasmic reticulum luminal domain maintains its inactive monomeric state through binding to the endoplasmic reticulum chaperone HSPA5/BiP. Upon the accumulation of misfolded proteins, the release of HSPA5/BiP allows the luminal domain to homodimerize, facilitating autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity. This endoribonuclease activity is specific to XBP1 mRNA, excising 26 nucleotides from the XBP1 transcript. The resulting spliced XBP1 transcript encodes a transcriptional activator that up-regulates the expression of UPR target genes. Beyond its role in the UPR, IRE1 acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional trafficking of CFTR to the cell membrane by modulating the expression and localization of SEC16A, highlighting its involvement in diverse cellular processes beyond the UPR.
1.The kinase activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Measured by its nuclease activity to cleave Xbp1 single stem-loop mini-substrate.
Technical Parameters
-
Species Human
-
Source Sf9 insect cells
-
Tag Tag Free
-
Accession
O75460-1 (P465-L977)
-
Molecular Construction
-
N-term
-
IRE1 (P465-L977)
Accession # O75460-1 -
C-term
-
-
Protein Length
Cytoplasmic Domain
-
Synonyms
ERN1; Ire1-Alpha; Endoplasmic Reticulum To Nucleus Signaling 1; HIRE1p; IRE1; IRE1a; Serine/Threonine-Protein Kinase/Endoribonuclease IRE1; Endoplasmic Reticulum-To-Nucleus Signaling 1; IRE1P; Non-Specific Serine/Threonine Protein Kinase; Inositol-Requiri
-
AA Sequence
PLSMHQQQQLQHQQFQKELEKIQLLQQQQQQLPFHPPGDTAQDGELLDTSGPYSESSGTSSPSTSPRASNHSLCSGSSASKAGSSPSLEQDDGDEETSVVIVGKISFCPKDVLGHGAEGTIVYRGMFDNRDVAVKRILPECFSFADREVQLLRESDEHPNVIRYFCTEKDRQFQYIAIELCAATLQEYVEQKDFAHLGLEPITLLQQTTSGLAHLHSLNIVHRDLKPHNILISMPNAHGKIKAMISDFGLCKKLAVGRHSFSRRSGVPGTEGWIAPEMLSEDCKENPTYTVDIFSAGCVFYYVISEGSHPFGKSLQRQANILLGACSLDCLHPEKHEDVIARELIEKMIAMDPQKRPSAKHVLKHPFFWSLEKQLQFFQDVSDRIEKESLDGPIVKQLERGGRAVVKMDWRENITVPLQTDLRKFRTYKGGSVRDLLRAMRNKKHHYRELPAEVRETLGSLPDDFVCYFTSRFPHLLAHTYRAMELCSHERLFQPYYFHEPPEPQPPVTPDAL
-
Predicted Molecular Mass
58.3 kDa
-
Molecular Weight
Approximately 65 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
-
Glycosylation
Yes
-
Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
1.Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4.
2.Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 7.5, 0.5 mM TCEP.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
-
Data Sheet (238 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
