1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Serine/Threonine Kinase Proteins
  4. Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1/IRE1)
  5. IRE1 Protein, Human (sf9)

IRE1 Protein, Human (sf9)

Cat. No.: HY-P73858
COA Handling Instructions

IRE1 is a key serine/threonine protein kinase and endoribonuclease essential for sensing the unfolded protein response (UPR) in the endoplasmic reticulum. In cellular homeostasis, it remains monomeric by binding to HSPA5/BiP. IRE1 Protein, Human (sf9) is the recombinant human-derived IRE1 protein, expressed by Sf9 insect cells , with tag free. The total length of IRE1 Protein, Human (sf9) is 513 a.a., with molecular weight of ~65 kDa.

For research use only. We do not sell to patients.

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Description

IRE1 is a key serine/threonine protein kinase and endoribonuclease essential for sensing the unfolded protein response (UPR) in the endoplasmic reticulum. In cellular homeostasis, it remains monomeric by binding to HSPA5/BiP. IRE1 Protein, Human (sf9) is the recombinant human-derived IRE1 protein, expressed by Sf9 insect cells , with tag free. The total length of IRE1 Protein, Human (sf9) is 513 a.a., with molecular weight of ~65 kDa.

Background

IRE1, a serine/threonine-protein kinase and endoribonuclease, serves as a pivotal sensor in the endoplasmic reticulum unfolded protein response (UPR). In the absence of cellular stress, the endoplasmic reticulum luminal domain maintains its inactive monomeric state through binding to the endoplasmic reticulum chaperone HSPA5/BiP. Upon the accumulation of misfolded proteins, the release of HSPA5/BiP allows the luminal domain to homodimerize, facilitating autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity. This endoribonuclease activity is specific to XBP1 mRNA, excising 26 nucleotides from the XBP1 transcript. The resulting spliced XBP1 transcript encodes a transcriptional activator that up-regulates the expression of UPR target genes. Beyond its role in the UPR, IRE1 acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional trafficking of CFTR to the cell membrane by modulating the expression and localization of SEC16A, highlighting its involvement in diverse cellular processes beyond the UPR.

Biological Activity

Measured by its nuclease activity to cleave Xbp1 single stem-loop mini-substrate.

Species

Human

Source

Sf9 insect cells

Tag

Tag Free

Accession

O75460 (P465-L977)

Gene ID
Synonyms
Serine/threonine-protein kinase/endoribonuclease IRE1; IRE1a; ERN1; IRE1
Molecular Weight

Approximately 65 kDa

Purity

Greater than 80% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IRE1 Protein, Human (sf9)
Cat. No.:
HY-P73858
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