IVD Protein, Mouse (sf9, His)
IVD protein, a vital component of the leucine catabolic pathway, facilitates the transformation of isovaleryl-CoA/3-methylbutanoyl-CoA into 3-methylbut-2-enoyl-CoA. This reaction acts as an intermediate step. Moreover, IVD protein demonstrates some capacity to oxidize saturated short-chain acyl-CoA thioesters like pentanoyl-CoA, hexenoyl-CoA, and butenoyl-CoA, albeit to a lesser degree. IVD Protein, Mouse (sf9, His) is the recombinant mouse-derived IVD protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Species: Mouse
- Source: Sf9 insect cells
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
IVD protein, a vital component of the leucine catabolic pathway, facilitates the transformation of isovaleryl-CoA/3-methylbutanoyl-CoA into 3-methylbut-2-enoyl-CoA. This reaction acts as an intermediate step. Moreover, IVD protein demonstrates some capacity to oxidize saturated short-chain acyl-CoA thioesters like pentanoyl-CoA, hexenoyl-CoA, and butenoyl-CoA, albeit to a lesser degree. IVD Protein, Mouse (sf9, His) is the recombinant mouse-derived IVD protein, expressed by Sf9 insect cells , with N-His labeled tag.
Background
The IVD protein plays a crucial role in the leucine (Leu) catabolic pathway by catalyzing the conversion of isovaleryl-CoA/3-methylbutanoyl-CoA to 3-methylbut-2-enoyl-CoA. This reaction serves as an intermediate step in the pathway. Additionally, the IVD protein exhibits the ability to catalyze the oxidation of other saturated short-chain acyl-CoA thioesters such as pentanoyl-CoA, hexenoyl-CoA, and butenoyl-CoA, although to a lesser extent.
Technical Parameters
-
Species Mouse
-
Source Sf9 insect cells
-
Tag N-His
-
Accession
Q9JHI5 (H31-R424)
-
Molecular Construction
-
N-term
-
His
-
IVD (H31-R424)
Accession # Q9JHI5 -
C-term
-
-
Protein Length
Full Length of Mature Protein
-
Synonyms
IVD; Isovaleryl Coenzyme A Dehydrogenase; Isovaleryl-CoA Dehydrogenase; Epididymis Secretory Sperm Binding Protein; Isovaleryl-CoA Dehydrogenase, Mitochondrial; Isovaleryl-CoA Dehydrogenase Isoform 3; Butyryl-CoA Dehydrogenase; Truncated Isovalery CoA Deh
-
AA Sequence
HSILPVDDDINGLNEEQKQLRHTISKFLQENLAPKAQEIDQTNDFKNLREFWKQLGSLGVLGITAPVQYGGSGLGYLEHVLVMEEISRASGAVGLSYGAHSNLCVNQIVRNGNEAQKEKYLPKLISGEFIGALAMSEPNAGSDVVSMKLKAEKKGDHYVLNGNKFWITNGPDADILVVYAKTDLTAVPASRGITAFIVEKGMPGFSTSKKLDKLGMRGSNTCELVFEDCKVPAANVLSQESKGVYVLMSGLDLERLVLAGGPLGIMQAVLDHTIPYLHVREAFGQKIGQFQLMQGKMADMYTRLMASRQYVYNVAKACDEGHIIPKDCAGVILYAAECATQVALDGIQCLGGNGYINDFPMGRFLRDAKLYEIGAGTSEVRRLVIGRAFNADFR
-
Predicted Molecular Mass
45.3 kDa
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
