KBTBD2 Protein, Human (sf9, His, StrepⅡ)

KBTBD2 in the BCR E3 ubiquitin ligase complex is a key regulator in the insulin signaling pathway and affects the insulin sensitivity of adipocytes. Its effects are manifested by regulating PIK3R1/p85alpha abundance by coordinating the ubiquitination process for PIK3R1 degradation. KBTBD2 Protein, Human (sf9, His, Strep) is the recombinant human-derived KBTBD2 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.

KBTBD2, functioning as a substrate-specific adapter within the BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex, emerges as a key regulator in the insulin signaling pathway with a pronounced impact on insulin sensitivity in adipocytes. This regulatory role is executed through the modulation of PIK3R1/p85alpha abundance, the regulatory subunit of phosphatidylinositol 3-kinase (PI3K). Notably, KBTBD2 orchestrates the ubiquitination process targeting PIK3R1 for 'Lys-48'-linked polyubiquitination, leading to its subsequent proteasome-mediated degradation. The intricate protein modification mechanism orchestrated by KBTBD2, specifically its role in protein ubiquitination, underscores its pivotal function in fine-tuning the insulin signaling cascade and, by extension, insulin sensitivity within adipocytes.

Human

Sf9 insect cells

N-StrepⅡ;N-8*His

Q8IY47 (S2-V623)

25948

Partial

STQDERQINTEYAVSLLEQLKLFYEQQLFTDIVLIVEGTEFPCHKMVLATCSSYFRAMFMSGLSESKQTHVHLRNVDAATLQIIITYAYTGNLAMNDSTVEQLYETACFLQVEDVLQRCREYLIKKINAENCVRLLSFADLFSCEELKQSAKRMVEHKFTAVYHQDAFMQLSHDLLIDILSSDNLNVEKEETVREAAMLWLEYNTESRSQYLSSVLSQIRIDALSEVTQRAWFQGLPPNDKSVVVQGLYKSMPKFFKPRLGMTKEEMMIFIEASSENPCSLYSSVCYSPQAEKVYKLCSPPADLHKVGTVVTPDNDIYIAGGQVPLKNTKTNHSKTSKLQTAFRTVNCFYWFDAQQNTWFPKTPMLFVRIKPSLVCCEGYIYAIGGDSVGGELNRRTVERYDTEKDEWTMVSPLPCAWQWSAAVVVHDCIYVMTLNLMYCYFPRSDSWVEMAMRQTSRSFASAAAFGDKIFYIGGLHIATNSGIRLPSGTVDGSSVTVEIYDVNKNEWKMAANIPAKRYSDPCVRAVVISNSLCVFMRETHLNERAKYVTYQYDLELDRWSLRQHISERVLWDLGRDFRCTVGKLYPSCLEESPWKPPTYLFSTDGTEEFELDGEMVALPPV

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.