KBTBD7 Protein, Human (sf9, His, StrepⅡ)
KBTBD7 is a component of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex and acts as a substrate linker to direct “Lys-48” ubiquitination and subsequent proteasomal degradation of the RAC1 guanine exchange factor TIAM1. This precise ubiquitination enables KBTBD7 to effectively regulate RAC1 signaling, affecting key biological processes such as cytoskeletal organization, cell migration, and cell proliferation. KBTBD7 Protein, Human (sf9, His, Strep) is the recombinant human-derived KBTBD7 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.
- Species: Human
- Source: Sf9 insect cells
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
KBTBD7 is a component of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex and acts as a substrate linker to direct “Lys-48” ubiquitination and subsequent proteasomal degradation of the RAC1 guanine exchange factor TIAM1. This precise ubiquitination enables KBTBD7 to effectively regulate RAC1 signaling, affecting key biological processes such as cytoskeletal organization, cell migration, and cell proliferation. KBTBD7 Protein, Human (sf9, His, Strep) is the recombinant human-derived KBTBD7 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.
Background
KBTBD7, a key constituent of the CUL3(KBTBD6/7) E3 ubiquitin ligase complex, serves as a substrate adapter with a pivotal role in orchestrating the 'Lys-48' ubiquitination and subsequent proteasomal degradation of the RAC1 guanine exchange factor (GEF) TIAM1. Through precise control of this ubiquitination process, KBTBD7 effectively modulates RAC1 signal transduction and influences various downstream biological processes, including cytoskeletal organization, cell migration, and cell proliferation. The ubiquitination of TIAM1 is contingent on the presence of the membrane-associated protein GABARAP, suggesting a potential local regulation of the complex's activity. With a primary role in protein ubiquitination, KBTBD7 plays a crucial part in fine-tuning cellular signaling pathways and regulating key cellular functions.
Technical Parameters
-
Species Human
-
Source Sf9 insect cells
-
Tag N-StrepⅡ;N-8*His
-
Accession
Q8WVZ9 (Q2-L684)
-
Gene ID84078
-
Molecular Construction
-
N-term
-
8*His-StrepⅡ
-
KBTBD7 (Q2-L684)
Accession # Q8WVZ9 -
C-term
-
-
Protein Length
Full Length
-
Synonyms
KBTBD7; Kelch Repeat And BTB (POZ) Domain Containing 7; Kelch Repeat And BTB Domain Containing 7; DKFZP434E2318; Kelch Repeat And BTB Domain-Containing Protein 7; KBTBD7 Protein
-
AA Sequence
QSREDVPRSRRLASPRGGRRPKRISKPSVSAFFTGPEELKDTAHSAALLAQLKSFYDARLLCDVTIEVVTPGSGPGTGRLFSCNRNVLAAACPYFKSMFTGGMYESQQASVTMHDVDAESFEVLVDYCYTGRVSLSEANVQRLYAASDMLQLEYVREACASFLARRLDLTNCTAILKFADAFDHHKLRSQAQSYIAHNFKQLSRMGSIREETLADLTLAQLLAVLRLDSLDIESERTVCHVAVQWLEAAAKERGPSAAEVFKCVRWMHFTEEDQDYLEGLLTKPIVKKYCLDVIEGALQMRYGDLLYKSLVPVPNSSSSSSSSNSLVSAAENPPQRLGMCAKEMVIFFGHPRDPFLCYDPYSGDIYTMPSPLTSFAHTKTVTSSAVCVSPDHDIYLAAQPRKDLWVYKPAQNSWQQLADRLLCREGMDVAYLNGYIYILGGRDPITGVKLKEVECYSVQRNQWALVAPVPHSFYSFELIVVQNYLYAVNSKRMLCYDPSHNMWLNCASLKRSDFQEACVFNDEIYCICDIPVMKVYNPARGEWRRISNIPLDSETHNYQIVNHDQKLLLITSTTPQWKKNRVTVYEYDTREDQWINIGTMLGLLQFDSGFICLCARVYPSCLEPGQSFITEEDDARSESSTEWDLDGFSELDSESGSSSSFSDDEVWVQVAPQRNAQDQQGSL
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
