LacI WT Protein, E.coli (His-SUMO)
LacI, the lactose operon repressor, regulates lactose metabolism by binding to the operator region and repressing gene transcription. The presence of the inducer, allolactose, alters its binding affinity, derepressing the operon. As a homotetramer, LacI finely tunes lactose metabolism, orchestrating regulatory mechanisms in response to the cellular environment. LacI WT Protein, E.coli (His-SUMO) is the recombinant E. coli-derived LacI protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Species: E.coli
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
LacI, the lactose operon repressor, regulates lactose metabolism by binding to the operator region and repressing gene transcription. The presence of the inducer, allolactose, alters its binding affinity, derepressing the operon. As a homotetramer, LacI finely tunes lactose metabolism, orchestrating regulatory mechanisms in response to the cellular environment. LacI WT Protein, E.coli (His-SUMO) is the recombinant E. coli-derived LacI protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
Background
LacI, the repressor of the lactose operon, plays a crucial role in the regulation of lactose metabolism. It functions by binding to the operator region and repressing the transcription of genes involved in lactose utilization. The binding affinity is altered in the presence of the inducer, allolactose, which leads to the derepression of the operon. As a homotetramer, LacI orchestrates the regulatory mechanisms that finely tune lactose metabolism in response to the cellular environment.
Technical Parameters
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Species E.coli
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Source E. coli
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Tag N-His;N-SUMO
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Accession
P03023 (M1-Q360)
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Gene ID945007 [NCBI]
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Molecular Construction
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N-term
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6*His-SUMO
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LacI (M1-Q360)
Accession # P03023 -
C-term
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Protein Length
Full Length
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Synonyms
TFPI; EPI; Prev. LACI; Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor); Extrinsic Pathway Inhibitor; Lipoprotein-Associated Coagulation Inhibitor; TFPI1; Anti-Convertin; Tissue Factor Pathway Inhibitor; TFI
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AA Sequence
MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ
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Predicted Molecular Mass
54.6 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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