LacI Protein, E.coli (His-SUMO)

LacI, the lactose operon repressor, regulates lactose metabolism by binding to the operator region and repressing gene transcription. The presence of the inducer, allolactose, alters its binding affinity, derepressing the operon. As a homotetramer, LacI finely tunes lactose metabolism, orchestrating regulatory mechanisms in response to the cellular environment. LacI Protein, E.coli (His-SUMO) is the recombinant E. coli-derived LacI protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of LacI Protein, E.coli (His-SUMO) is 360 a.a., with molecular weight of ~54.6 kDa.

LacI, the repressor of the lactose operon, plays a crucial role in the regulation of lactose metabolism. It functions by binding to the operator region and repressing the transcription of genes involved in lactose utilization. The binding affinity is altered in the presence of the inducer, allolactose, which leads to the derepression of the operon. As a homotetramer, LacI orchestrates the regulatory mechanisms that finely tune lactose metabolism in response to the cellular environment.

E.coli

E. coli

N-His;N-SUMO

P03023 (1M-360Q)

945007  [NCBI]

MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ

Approximately 54.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.