MAOA Protein, Human (Active, sf9, His)
Based on 1 Customer Validation
MAOA Protein, Human (Active, sf9, His) is the recombinant human-derived MAOA protein, expressed by Sf9 insect cells, with N-His tag. This product contains detergent and is not suitable for cell-related experiments.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Biological Activity
Description
MAOA Protein, Human (Active, sf9, His) is the recombinant human-derived MAOA protein, expressed by Sf9 insect cells, with N-His tag. This product contains detergent and is not suitable for cell-related experiments.
Background
Monoamine Oxidase A (MAO-A) catalyzes the oxidative deamination of primary and some secondary amines, such as neurotransmitters, while reducing oxygen to hydrogen peroxide. It plays a crucial role in metabolizing neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAO-A preferentially oxidizes serotonin. Additionally, it catalyzes the oxidative deamination of kynuramine to form 3-(2-aminophenyl)-3-oxopropanal, which can spontaneously condense into 4-hydroxyquinoline (by similar).
Verified Bioactivity
Measured by its ability to produce hydrogen peroxide during the oxidation of Tyramine. The specific activity is >300 pmol/min/ug.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His
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Accession
P21397-1 (E2-S527)
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Gene ID4128
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Molecular Construction
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N-term
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His
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MAOA (E2-S527)
Accession # P21397-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
MAOA; MAO-A; Monoamine Oxidase A; Amine Oxidase; Amine Oxidase [Flavin-Containing] A; BRNRS; Monoamine Oxidase Type A
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AA Sequence
ENQEKASIAGHMFDVVVIGGGISGLSAAKLLTEYGVSVLVLEARDRVGGRTYTIRNEHVDYVDVGGAYVGPTQNRILRLSKELGIETYKVNVSERLVQYVKGKTYPFRGAFPPVWNPIAYLDYNNLWRTIDNMGKEIPTDAPWEAQHADKWDKMTMKELIDKICWTKTARRFAYLFVNINVTSEPHEVSALWFLWYVKQCGGTTRIFSVTNGGQERKFVGGSGQVSERIMDLLGDQVKLNHPVTHVDQSSDNIIIETLNHEHYECKYVINAIPPTLTAKIHFRPELPAERNQLIQRLPMGAVIKCMMYYKEAFWKKKDYCGCMIIEDEDAPISITLDDTKPDGSLPAIMGFILARKADRLAKLHKEIRKKKICELYAKVLGSQEALHPVHYEEKNWCEEQYSGGCYTAYFPPGIMTQYGRVIRQPVGRIFFAGTETATKWSGYMEGAVEAGERAAREVLNGLGKVTEKDIWVQEPESKDVPAVEITHTFWERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS
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Predicted Molecular Mass
61.6 kDa
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Molecular Weight
Approximately 60 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 0.05% DDM, 0.01% CHS, pH7.5 with glycerol as protectant. Due to the cytotoxicity of the detergent, this product is not appropriate for cell-based experiments.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)