MAOA Protein, Human (Active, sf9, His)

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MAOA Protein, Human (Active, sf9, His) is the recombinant human-derived MAOA protein, expressed by Sf9 insect cells, with N-His tag. This product contains detergent and is not suitable for cell-related experiments.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

MAOA Protein, Human (Active, sf9, His) is the recombinant human-derived MAOA protein, expressed by Sf9 insect cells, with N-His tag. This product contains detergent and is not suitable for cell-related experiments.

Background

Monoamine Oxidase A (MAO-A) catalyzes the oxidative deamination of primary and some secondary amines, such as neurotransmitters, while reducing oxygen to hydrogen peroxide. It plays a crucial role in metabolizing neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAO-A preferentially oxidizes serotonin. Additionally, it catalyzes the oxidative deamination of kynuramine to form 3-(2-aminophenyl)-3-oxopropanal, which can spontaneously condense into 4-hydroxyquinoline (by similar).

Verified Bioactivity

Measured by its ability to produce hydrogen peroxide during the oxidation of Tyramine. The specific activity is >300 pmol/min/ug.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag N-His
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • His
    • MAOA (E2-S527)
      Accession # P21397-1
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    MAOA; MAO-A; Monoamine Oxidase A; Amine Oxidase; Amine Oxidase [Flavin-Containing] A; BRNRS; Monoamine Oxidase Type A

  • AA Sequence

    ENQEKASIAGHMFDVVVIGGGISGLSAAKLLTEYGVSVLVLEARDRVGGRTYTIRNEHVDYVDVGGAYVGPTQNRILRLSKELGIETYKVNVSERLVQYVKGKTYPFRGAFPPVWNPIAYLDYNNLWRTIDNMGKEIPTDAPWEAQHADKWDKMTMKELIDKICWTKTARRFAYLFVNINVTSEPHEVSALWFLWYVKQCGGTTRIFSVTNGGQERKFVGGSGQVSERIMDLLGDQVKLNHPVTHVDQSSDNIIIETLNHEHYECKYVINAIPPTLTAKIHFRPELPAERNQLIQRLPMGAVIKCMMYYKEAFWKKKDYCGCMIIEDEDAPISITLDDTKPDGSLPAIMGFILARKADRLAKLHKEIRKKKICELYAKVLGSQEALHPVHYEEKNWCEEQYSGGCYTAYFPPGIMTQYGRVIRQPVGRIFFAGTETATKWSGYMEGAVEAGERAAREVLNGLGKVTEKDIWVQEPESKDVPAVEITHTFWERNLPSVSGLLKIIGFSTSVTALGFVLYKYKLLPRS

  • Predicted Molecular Mass

    61.6 kDa

  • Molecular Weight

    Approximately 60 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 0.05% DDM, 0.01% CHS, pH7.5 with glycerol as protectant. Due to the cytotoxicity of the detergent, this product is not appropriate for cell-based experiments.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
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Concentration (final) Concentration (final)
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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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