MetC Protein, E.coli (His)
MetC protein is a multifunctional enzyme that catalyzes the cleavage of cystathionine to homocysteine, pyruvate, and ammonia, which is essential for methionine biosynthesis. It also acts as a cysteine desulfurase, converting cysteine into sulfide. MetC Protein, E.coli (His) is the recombinant E. coli-derived MetC protein, expressed by E. coli , with N-His labeled tag.
- Species: E.coli
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
MetC protein is a multifunctional enzyme that catalyzes the cleavage of cystathionine to homocysteine, pyruvate, and ammonia, which is essential for methionine biosynthesis. It also acts as a cysteine desulfurase, converting cysteine into sulfide. MetC Protein, E.coli (His) is the recombinant E. coli-derived MetC protein, expressed by E. coli , with N-His labeled tag.
Background
The MetC protein serves as a multifunctional enzyme with a primary role in catalyzing the cleavage of cystathionine to homocysteine, pyruvate, and ammonia, a critical step in methionine biosynthesis. Beyond its primary function, MetC demonstrates cysteine desulfhydrase activity, converting cysteine into sulfide. Moreover, under specific growth conditions, the protein exhibits noteworthy coactivity as an alanine racemase. This versatile enzymatic repertoire highlights MetC's involvement in diverse metabolic pathways, showcasing its capacity to contribute to the intricate regulation of amino acid metabolism and biosynthetic processes.
Technical Parameters
-
Species E.coli
-
Source E. coli
-
Tag N-His
-
Accession
P06721 (M1-A392)
-
Gene ID946240 [NCBI]
-
Molecular Construction
-
N-term
-
His
-
MetC (M1-A392)
Accession # P06721 -
C-term
-
-
Protein Length
Full Length of Cystathionine beta-lyase MetC Chain
-
Synonyms
Cystathionine beta-lyase MetC; EC:4.4.1.13; CBL; CL ; Beta-cystathionase MetC; Cysteine desulfhydrase MetC; CD; EC:4.4.1.28; Cysteine lyase MetC; Cysteine-S-conjugate beta-lyase MetC; metC; b3008; JW2975
-
AA Sequence
MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKHATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFIEQGDHVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSITMEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDAMIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEWLAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLFSMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
-
Predicted Molecular Mass
46.8 kDa
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
