nanA Protein, S. haemolyticus

nanA Protein, S. haemolyticus is the recombinant nanA protein, expressed by E. coli, with tag free tag.

For research use only. We do not sell to patients.
  • Species: Others
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

nanA Protein, S. haemolyticus is the recombinant nanA protein, expressed by E. coli, with tag free tag.

Background

nanA is an enzyme that catalyzes the reversible aldol cleavage of N-acetylneuraminic acid (sialic acid; Neu5Ac) into pyruvate and N-acetylmannosamine (ManNAc) via a Schiff base intermediate.

Technical Parameters

  • Species Others
  • Source E. coli
  • Tag Tag Free
  • Accession
  • Gene ID
  • Protein Length

    Full Length

  • Synonyms

    N-acetylneuraminate lyase; EC:4.1.3.3; NA; NAL; Neu5Ac lyase ; N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; Sialate lyase; Sialic acid aldolase; Sialic acid lyase; nanA; SH0282;

  • AA Sequence

    MEDKLKGLYAALLVPFDEHGQVKEEGLKQIAKNAIETEKLDGLYVNGSSGENFLISKEQKKQIFRVVKETVGDDVNLIAQIGSLDLNEAIELGKYATELGYDALSAVTPFYYPFSFEEIKQYYFDIIEATQNNMIIYAIPDLTGVNISIEQFGELFNHEKVVGVKYTAPNFFLLERIRKAFPNKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRARQIFDLAREGKIAEAYQIQHDTNDIIETVLSMGIYPTLKEILKSRGIDGGLPKRPFRPFDESHRDALDTLIKKYDL

  • Predicted Molecular Mass

    33.9 kDa

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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