NCL Protein, Human (HEK293, mFc)
Nucleolar protein (NCL) is the major nucleolar protein in actively growing eukaryotic cells and is associated with intranucleolar chromatin and preribosomal granules. It induces chromatin decondensation by binding to histone H1 and plays a role in pre-rRNA transcription, ribosome assembly, and potential transcription elongation. NCL Protein, Human (HEK293, mFc) is the recombinant human-derived NCL protein, expressed by HEK293, with C-mFc labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Nucleolar protein (NCL) is the major nucleolar protein in actively growing eukaryotic cells and is associated with intranucleolar chromatin and preribosomal granules. It induces chromatin decondensation by binding to histone H1 and plays a role in pre-rRNA transcription, ribosome assembly, and potential transcription elongation. NCL Protein, Human (HEK293, mFc) is the recombinant human-derived NCL protein, expressed by HEK293, with C-mFc labeled tag.
Background
Nucleolin (NCL) serves as the major nucleolar protein in actively growing eukaryotic cells, associating with intranucleolar chromatin and pre-ribosomal particles. Its involvement in inducing chromatin decondensation is facilitated by binding to histone H1. Nucleolin is implicated in pre-rRNA transcription, ribosome assembly, and potentially plays a role in transcriptional elongation. It exhibits a higher affinity for RNA oligonucleotides containing 5'-UUAGGG-3' repeats compared to telomeric single-stranded DNA with 5'-TTAGGG-3' repeats. Additionally, NCL is identified in an IGF2BP1-dependent mRNP granule complex. It is part of the SWAP complex and a larger complex involving HTATSF1, CDK9, CCNT1, RNA polymerase II, SUPT5H, and Nucleolin. NCL engages in diverse interactions with proteins such as AICDA, APTX, C1QBP, ERBB4, FMR1, GZF1, NSUN2, NVL, SETX, TERT, WDR46, ZFP36, LRRC34, RRP1B, HNRNPU, RIOK1, ZBTB7B, MDK, and HDGF, highlighting its multifaceted roles in various cellular processes.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-mFc
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Accession
P19338 (V2-E710)
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Gene ID4691
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Molecular Construction
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N-term
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NCL (V2-E710)
Accession # P19338 -
mFc
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C-term
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Protein Length
Full Length
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Synonyms
NUCLEOLIN; Nsr1; Prev. NCL; Protein C23; Nucleolin; Nucleolin Multifunctional Protein; C23; FLJ45706; MS1116 ; Nucl
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AA Sequence
VKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQKKGKKAAATSAKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPAKAVTTPGKKGATPGKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDEDEDEDEIEPAAMKAAAAAPASEDEDDEDDEDDEDDDDDEEDDSEEEAMETTPAKGKKAAKVVPVKAKNVAEDEDEEEDDEDEDDDDDEDDEDDDDEDDEEEEEEEEEEPVKEAPGKRKKEMAKQKAAPEAKKQKVEGTEPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLPYKVTQDELKEVFEDAAEIRLVSKDGKSKGIAYIEFKTEADAEKTFEEKQGTEIDGRSISLYYTGEKGQNQDYRGGKNSTWSGESKTLVLSNLSYSATEETLQEVFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEALNSCNKREIEGRAIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE
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Predicted Molecular Mass
103.5 kDa
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Molecular Weight
Approximately 130-150 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (240 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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