NELL2 Protein, Human (sf9, His)

NELL2 protein, crucial for neuron survival, modulates MAPK pathways and regulates hypothalamic gonadotropin-releasing hormone (GNRH) secretion, impacting puberty control. NELL2 forms homotrimers and engages with PKC beta-1, indicating intricate molecular interactions in its regulatory functions. NELL2 Protein, Human (sf9, His) is the recombinant human-derived NELL2 protein, expressed by Sf9 insect cells , with C-His labeled tag.

NELL2 protein is indispensable for neuron survival, acting through the modulation of MAPK pathways. It plays a crucial role in the regulation of hypothalamic gonadotropin-releasing hormone (GNRH) secretion and is implicated in the control of puberty. Structurally, NELL2 forms homotrimers, and its functional associations extend to the binding of PKC beta-1, suggesting intricate molecular interactions involved in its regulatory roles.

Measured by the ability of the immobilized protein to support the adhesion of C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.2894 μg/mL, corresponding to a specific activity is 3455.425 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.2894 μg/mL, corresponding to a specific activity is 3455.425 units/mg.

Human

Sf9 insect cells

C-His

Q99435 (L22-L816)

4753  [NCBI]

Full Length of Mature Protein

LGVDPSLQIDVLTELELGESTTGVRQVPGLHNGTKAFLFQDTPRSIKASTATAEQFFQKLRNKHEFTILVTLKQTHLNSGVILSIHHLDHRYLELESSGHRNEVRLHYRSGSHRPHTEVFPYILADDKWHKLSLAISASHLILHIDCNKIYERVVEKPSTDLPLGTTFWLGQRNNAHGYFKGIMQDVQLLVMPQGFIAQCPDLNRTCPTCNDFHGLVQKIMELQDILAKTSAKLSRAEQRMNRLDQCYCERTCTMKGTTYREFESWIDGCKNCTCLNGTIQCETLICPNPDCPLKSALAYVDGKCCKECKSICQFQGRTYFEGERNTVYSSSGVCVLYECKDQTMKLVESSGCPALDCPESHQITLSHSCCKVCKGYDFCSERHNCMENSICRNLNDRAVCSCRDGFRALREDNAYCEDIDECAEGRHYCRENTMCVNTPGSFMCICKTGYIRIDDYSCTEHDECITNQHNCDENALCFNTVGGHNCVCKPGYTGNGTTCKAFCKDGCRNGGACIAANVCACPQGFTGPSCETDIDECSDGFVQCDSRANCINLPGWYHCECRDGYHDNGMFSPSGESCEDIDECGTGRHSCANDTICFNLDGGYDCRCPHGKNCTGDCIHDGKVKHNGQIWVLENDRCSVCSCQNGFVMCRRMVCDCENPTVDLFCCPECDPRLSSQCLHQNGETLYNSGDTWVQNCQQCRCLQGEVDCWPLPCPDVECEFSILPENECCPRCVTDPCQADTIRNDITKTCLDEMNVVRFTGSSWIKHGTECTLCQCKNGHICCSVDPQCLQEL

Approximately 110 kDa

≥ 85%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of PBS, 10% glycerol, pH 7.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.