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NELL2 Protein, Human (sf9, His)

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Based on 1 Customer Validation

NELL2 protein, crucial for neuron survival, modulates MAPK pathways and regulates hypothalamic gonadotropin-releasing hormone (GNRH) secretion, impacting puberty control. NELL2 forms homotrimers and engages with PKC beta-1, indicating intricate molecular interactions in its regulatory functions. NELL2 Protein, Human (sf9, His) is the recombinant human-derived NELL2 protein, expressed by Sf9 insect cells , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for NELL2 Protein, Human (sf9, His)
  • NELL2 Protein, Human (sf9, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

NELL2 protein, crucial for neuron survival, modulates MAPK pathways and regulates hypothalamic gonadotropin-releasing hormone (GNRH) secretion, impacting puberty control. NELL2 forms homotrimers and engages with PKC beta-1, indicating intricate molecular interactions in its regulatory functions. NELL2 Protein, Human (sf9, His) is the recombinant human-derived NELL2 protein, expressed by Sf9 insect cells , with C-His labeled tag.

Background

NELL2 protein is indispensable for neuron survival, acting through the modulation of MAPK pathways. It plays a crucial role in the regulation of hypothalamic gonadotropin-releasing hormone (GNRH) secretion and is implicated in the control of puberty. Structurally, NELL2 forms homotrimers, and its functional associations extend to the binding of PKC beta-1, suggesting intricate molecular interactions involved in its regulatory roles.

Verified Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.2894 μg/mL, corresponding to a specific activity is 3455.425 units/mg.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag C-His
  • Accession

    Q99435 (L22-L816)

  • Gene ID
    4753  [NCBI]
  • Molecular Construction
    • N-term
    • NELL2 (L22-L816)
      Accession # Q99435
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    NELL2; Alternative Protein NELL2; Neural EGFL Like 2; NEL-Related Protein 2; NRP2; Nel-Related Protein 2; Protein Kinase C-Binding Protein NELL2; Nel (Chicken)-Like 2; NEL-Like Protein 2; NEL-Like 2 (Chicken); Neural Epidermal Growth Factor-Like 2

  • AA Sequence

    LGVDPSLQIDVLTELELGESTTGVRQVPGLHNGTKAFLFQDTPRSIKASTATAEQFFQKLRNKHEFTILVTLKQTHLNSGVILSIHHLDHRYLELESSGHRNEVRLHYRSGSHRPHTEVFPYILADDKWHKLSLAISASHLILHIDCNKIYERVVEKPSTDLPLGTTFWLGQRNNAHGYFKGIMQDVQLLVMPQGFIAQCPDLNRTCPTCNDFHGLVQKIMELQDILAKTSAKLSRAEQRMNRLDQCYCERTCTMKGTTYREFESWIDGCKNCTCLNGTIQCETLICPNPDCPLKSALAYVDGKCCKECKSICQFQGRTYFEGERNTVYSSSGVCVLYECKDQTMKLVESSGCPALDCPESHQITLSHSCCKVCKGYDFCSERHNCMENSICRNLNDRAVCSCRDGFRALREDNAYCEDIDECAEGRHYCRENTMCVNTPGSFMCICKTGYIRIDDYSCTEHDECITNQHNCDENALCFNTVGGHNCVCKPGYTGNGTTCKAFCKDGCRNGGACIAANVCACPQGFTGPSCETDIDECSDGFVQCDSRANCINLPGWYHCECRDGYHDNGMFSPSGESCEDIDECGTGRHSCANDTICFNLDGGYDCRCPHGKNCTGDCIHDGKVKHNGQIWVLENDRCSVCSCQNGFVMCRRMVCDCENPTVDLFCCPECDPRLSSQCLHQNGETLYNSGDTWVQNCQQCRCLQGEVDCWPLPCPDVECEFSILPENECCPRCVTDPCQADTIRNDITKTCLDEMNVVRFTGSSWIKHGTECTLCQCKNGHICCSVDPQCLQEL

  • Predicted Molecular Mass

    90.4 kDa

  • Molecular Weight

    Approximately 110 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 85%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of PBS, 10% glycerol, pH 7.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Measured by the ability of the immobilized protein to support the adhesion of C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.2894 μg/mL, corresponding to a specific activity is 3455.425 units/mg.

  • NELL2 Protein, Human (sf9, His) Product Image
    1/16
  • Experimental Validation Results for NELL2 Protein, Human (sf9, His)
  • NELL2 Protein, Human (sf9, His) Product Image