OGG1 Protein, Human (N-His)

The OGG1 protein is a DNA repair enzyme specifically designed to cleave DNA at the 8-oxoG residue and plays a key role in maintaining genome integrity. It demonstrates the ability to excise damaged bases such as 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine from the DNA structure (FAPY). OGG1 Protein, Human (N-His) is the recombinant human-derived OGG1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of OGG1 Protein, Human (N-His) is 344 a.a., with molecular weight of ~38.7 kDa.

OGG1 (8-Oxoguanine DNA Glycosylase 1) is a DNA repair enzyme crucial for maintaining genomic integrity. Specializing in the repair of oxidative DNA damage, OGG1 specifically incises DNA at 8-oxoG (8-oxoguanine) residues. Additionally, it excises other oxidatively damaged bases, such as 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY), contributing to the removal of mutagenic lesions from the DNA. Notably, OGG1 possesses a beta-lyase activity, enabling it to nick the DNA strand 3' to the lesion, initiating the repair process. This enzymatic versatility highlights OGG1's critical role in safeguarding the genome by counteracting the detrimental effects of oxidative stress on DNA integrity.

Data is not available.

Human

E. coli

N-6*His

O15527-1 (P2-G345)

4968

PARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQAVLFSADLRQSRHAQEPPAKRRKGSKGPEG

Approximately 38.7 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4, 40% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.