1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. OGG1 Protein, Human (His)

OGG1 Protein, Human (His)

Cat. No.: HY-P72338
COA Handling Instructions

The OGG1 protein is a DNA repair enzyme specifically designed to cleave DNA at the 8-oxoG residue and plays a key role in maintaining genome integrity. It demonstrates the ability to excise damaged bases such as 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine from the DNA structure (FAPY). OGG1 Protein, Human (His) is the recombinant human-derived OGG1 protein, expressed by E. coli , with C-6*His labeled tag. The total length of OGG1 Protein, Human (His) is 344 a.a., with molecular weight of ~38.7 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

The OGG1 protein is a DNA repair enzyme specifically designed to cleave DNA at the 8-oxoG residue and plays a key role in maintaining genome integrity. It demonstrates the ability to excise damaged bases such as 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine from the DNA structure (FAPY). OGG1 Protein, Human (His) is the recombinant human-derived OGG1 protein, expressed by E. coli , with C-6*His labeled tag. The total length of OGG1 Protein, Human (His) is 344 a.a., with molecular weight of ~38.7 kDa.

Background

OGG1 (8-Oxoguanine DNA Glycosylase 1) is a DNA repair enzyme crucial for maintaining genomic integrity. Specializing in the repair of oxidative DNA damage, OGG1 specifically incises DNA at 8-oxoG (8-oxoguanine) residues. Additionally, it excises other oxidatively damaged bases, such as 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY), contributing to the removal of mutagenic lesions from the DNA. Notably, OGG1 possesses a beta-lyase activity, enabling it to nick the DNA strand 3' to the lesion, initiating the repair process. This enzymatic versatility highlights OGG1's critical role in safeguarding the genome by counteracting the detrimental effects of oxidative stress on DNA integrity.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

E. coli

Tag

C-6*His

Accession

O15527 (P2-G345)

Gene ID
Molecular Construction
N-term
OGG1 (P2-G345)
Accession # O15527
6*His
C-term
Synonyms
8-oxoguanine DNA glycosylase; 8-hydroxyguanine DNA glycosylase; MMH; MUTM; OGH1
AA Sequence

PARALLPRRMGHRTLASTPALWASIPCPRSELRLDLVLPSGQSFRWREQSPAHWSGVLADQVWTLTQTEEQLHCTVYRGDKSQASRPTPDELEAVRKYFQLDVTLAQLYHHWGSVDSHFQEVAQKFQGVRLLRQDPIECLFSFICSSNNNIARITGMVERLCQAFGPRLIQLDDVTYHGFPSLQALAGPEVEAHLRKLGLGYRARYVSASARAILEEQGGLAWLQQLRESSYEEAHKALCILPGVGTKVADCICLMALDKPQAVPVDVHMWHIAQRDYSWHPTTSQAKGPSPQTNKELGNFFRSLWGPYAGWAQAVLFSADLRQSRHAQEPPAKRRKGSKGPEG

Molecular Weight

Approximately 38.7 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris HCl, 50 mM NaCl, 1 mM EDTA, 40% Glycerol, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

OGG1 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
OGG1 Protein, Human (His)
Cat. No.:
HY-P72338
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