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PDGF-DD Protein, Human (HEK293, His)

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The PDGF-DD protein is a key growth factor with critical regulatory effects on embryonic development, cell proliferation, migration, survival, and chemotaxis. It is a potent mitogen for mesenchymal cells and plays a crucial role in wound healing. PDGF-DD Protein, Human (HEK293, His) is the recombinant cynomolgus-derived PDGF-DD protein, expressed by HEK293 , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The PDGF-DD protein is a key growth factor with critical regulatory effects on embryonic development, cell proliferation, migration, survival, and chemotaxis. It is a potent mitogen for mesenchymal cells and plays a crucial role in wound healing. PDGF-DD Protein, Human (HEK293, His) is the recombinant cynomolgus-derived PDGF-DD protein, expressed by HEK293 , with N-His labeled tag.

Background

PDGF-DD protein functions as a crucial growth factor with diverse roles in embryonic development, cell proliferation, migration, survival, and chemotaxis. It serves as a potent mitogen for mesenchymal cells and plays a pivotal role in wound healing processes. Moreover, PDGF-DD is involved in orchestrating events critical for angiogenesis, including macrophage recruitment, increased interstitial pressure, and blood vessel maturation. Its ability to initiate processes leading to mesangial proliferative glomerulonephritis, characterized by monocyte and macrophage influx along with extracellular matrix production, underscores its regulatory impact in pathophysiological conditions. The protein forms homodimers through disulfide linkages and interacts with PDGFRB homodimers, as well as heterodimers formed by PDGFRA and PDGFRB, illustrating its complex engagement in signaling pathways.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-8*His
  • Accession

    Q9GZP0-1 (R19-R370)

  • Gene ID
    80310
  • Molecular Construction
    • N-term
    • 8*His
    • PDGF-DD (R19-R370)
      Accession # Q9GZP0-1
    • C-term
  • Protein Length

    Full Length of Isoform-1 Mature Protein

  • Synonyms

    PDGFD; Platelet-Derived Growth Factor D; Platelet Derived Growth Factor D; Iris-Expressed Growth Factor; SCDGF-B; SCDGFB; IEGF; PDGF-D; MSTP036; Spinal Cord Derived Growth Factor B; Spinal Cord-Derived Growth Factor B

  • AA Sequence

    RDTSATPQSASIKALRNANLRRDESNHLTDLYRRDETIQVKGNGYVQSPRFPNSYPRNLLLTWRLHSQENTRIQLVFDNQFGLEEAENDICRYDFVEVEDISETSTIIRGRWCGHKEVPPRIKSRTNQIKITFKSDDYFVAKPGFKIYYSLLEDFQPAAASETNWESVTSSISGVSYNSPSVTDPTLIADALDKKIAEFDTVEDLLKYFNPESWQEDLENMYLDTPRYRGRSYHDRKSKVDLDRLNDDAKRYSCTPRNYSVNIREELKLANVVFFPRCLLVQRCGGNCGCGTVNWRSCTCNSGKTVKKYHEVLQFEPGHIKRRGRAKTMALVDIQLDHHERCDCICSSRPPR

  • Predicted Molecular Mass

    41.8 kDa

  • Molecular Weight

    Approximately 50-65 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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