PIGR Protein, Mouse (HEK293, His)

PIGR proteins mediate the selective transcytosis of polymerized IgA and IgM across mucosal epithelial cells. It binds to these immunoglobulins at the basolateral surface, forming complexes that are transported intracellularly and secreted at the apical surface. PIGR Protein, Mouse (HEK293, His) is the recombinant mouse-derived PIGR protein, expressed by HEK293 , with C-His labeled tag. The total length of PIGR Protein, Mouse (HEK293, His) is 627 a.a., with molecular weight of 90-100 kDa.

The PIGR protein serves as a crucial mediator in the selective transcytosis of polymeric IgA and IgM across mucosal epithelial cells. At the basolateral surface of epithelial cells, it binds to polymeric IgA and IgM, forming a complex that undergoes transportation across the cell to be ultimately secreted at the apical surface. Throughout this transcytosis process, a cleavage event separates the extracellular segment, known as the secretory component, from the transmembrane segment. PIGR, through its N-linked glycans, ensures the anchoring of secretory IgA (sIgA) molecules to the mucus lining the epithelial surface, contributing to the neutralization of extracellular pathogens. In its free form, PIGR may also act as a non-specific microbial scavenger, playing a role in preventing pathogen interaction with epithelial cells.

1.Measured by its binding ability in a functional ELISA. When Recombinant Mouse plgR is coated at 2 μg/mL (100 μL/well), the concentration of mouse IgM that produces 50% of the optimal binding response is 297.7 ng/mL.
2.Measured by its binding ability in a functional ELISA . Immobilized recombinant mouse PIGR at 5 μg/mL (100 μl/well) can bind mouse IgM with a linear range of 0.156-10 μg/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Mouse plgR is coated at 2 μg/mL (100 μL/well), the concentration of mouse IgM that produces 50% of the optimal binding response is 297.7 ng/mL.

Mouse

HEK293

C-His

O70570 (K19-K645)

18703  [NCBI]

KSPIFGPQEVSSIEGDSVSITCYYPDTSVNRHTRKYWCRQGASGMCTTLISSNGYLSKEYSGRANLINFPENNTFVINIEQLTQDDTGSYKCGLGTSNRGLSFDVSLEVSQVPELPSDTHVYTKDIGRNVTIECPFKRENAPSKKSLCKKTNQSCELVIDSTEKVNPSYIGRAKLFMKGTDLTVFYVNISHLTHNDAGLYICQAGEGPSADKKNVDLQVLAPEPELLYKDLRSSVTFECDLGREVANEAKYLCRMNKETCDVIINTLGKRDPDFEGRILITPKDDNGRFSVLITGLRKEDAGHYQCGAHSSGLPQEGWPIQTWQLFVNEESTIPNRRSVVKGVTGGSVAIACPYNPKESSSLKYWCRWEGDGNGHCPVLVGTQAQVQEEYEGRLALFDQPGNGTYTVILNQLTTEDAGFYWCLTNGDSRWRTTIELQVAEATREPNLEVTPQNATAVLGETFTVSCHYPCKFYSQEKYWCKWSNKGCHILPSHDEGARQSSVSCDQSSQLVSMTLNPVSKEDEGWYWCGVKQGQTYGETTAIYIAVEERTRGSSHVNPTDANARAKVALEEEVVDSSISEKENKAIPNPGPFANEREIQNVGDQAQENRASGDAGSADGQSRSSSSK

90-100 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.