PIM2 Protein, Human (Active)
Based on 1 Customer Validation
The PIM2 protein is a proto-oncogene with serine/threonine kinase activity that critically promotes cell survival and proliferation through multiple mechanisms. It enhances MYC stability and transcriptional activity, explaining the synergistic effect in tumorigenesis. PIM2 Protein, Human is the recombinant human-derived PIM2 protein, expressed by E. coli , with tag free.
- Species: Human
- Source: E. coli
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
The PIM2 protein is a proto-oncogene with serine/threonine kinase activity that critically promotes cell survival and proliferation through multiple mechanisms. It enhances MYC stability and transcriptional activity, explaining the synergistic effect in tumorigenesis. PIM2 Protein, Human is the recombinant human-derived PIM2 protein, expressed by E. coli , with tag free.
The PIM2 protein, a proto-oncogene with serine/threonine kinase activity, plays a crucial role in cell survival and proliferation by exerting its oncogenic activity through multiple mechanisms. It regulates MYC transcriptional activity, cell cycle progression, and cap-dependent protein translation. Phosphorylation of MYC by PIM2 enhances MYC protein stability, resulting in increased transcriptional activity and potentially explaining the robust synergism between these two oncogenes in tumorigenesis. PIM2 also mediates survival signaling by phosphorylating the pro-apoptotic protein BAD, leading to the release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Moreover, PIM2 positively regulates the I-kappa-B kinase/NF-kappa-B cascade, promoting cell survival in response to various proliferative signals. It facilitates growth factor-independent proliferation by phosphorylating cell cycle factors such as CDKN1A and CDKN1B. Additionally, PIM2 is involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecencelabeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device.
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag Tag Free
-
Accession
Q9P1W9 (M1-P311)
-
Gene ID11040
-
Molecular Construction
-
N-term
-
PIM2 (M1-P311)
Accession # Q9P1W9 -
C-term
-
-
Protein Length
Full Length
-
Synonyms
PIM2; Pim-2h; Pim-2 Proto-Oncogene, Serine/Threonine Kinase; Proto-Oncogene Pim-2 (Serine Threonine Kinase); Serine/Threonine-Protein Kinase Pim-2; Non-Specific Serine/Threonine Protein Kinase; Pim-2 Oncogene
-
AA Sequence
MLTKPLQGPPAPPGTPTPPPGGKDREAFEAEYRLGPLLGKGGFGTVFAGHRLTDRLQVAIKVIPRNRVLGWSPLSDSVTCPLEVALLWKVGAGGGHPGVIRLLDWFETQEGFMLVLERPLPAQDLFDYITEKGPLGEGPSRCFFGQVVAAIQHCHSRGVVHRDIKDENILIDLRRGCAKLIDFGSGALLHDEPYTDFDGTRVYSPPEWISRHQYHALPATVWSLGILLYDMVCGDIPFERDQEILEAELHFPAHVSPDCCALIRRCLAPKPSSRPSLEEILLDPWMQTPAEDVPLNPSKGGPAPLAWSLLP
-
Predicted Molecular Mass
34.2 kDa
-
Molecular Weight
Approximately 34 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 300 mM NaCl, 5% glycerol, 0.5 mM TCEP.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
