PLCD4 Protein, Human (sf9, GST)
PLCD4 Protein, Human (sf9, GST) is the recombinant human-derived PLCD4, expressed by Sf9 insect cells , with GST labeled tag. ,
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
PLCD4 Protein, Human (sf9, GST) is the recombinant human-derived PLCD4, expressed by Sf9 insect cells , with GST labeled tag. ,
PLCD4 is a phospholipase that hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two second messenger molecules, diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 triggers the release of Ca2+ from intracellular stores. During fertilization, PLCD4 is essential for the acrosome reaction in sperm, likely by serving as a key enzyme for intracellular Ca2+ mobilization in the zona pellucida-induced acrosome reaction. It may also play a role in cell growth and collaborates with nuclear PKC to modulate liver regeneration. Overexpression of PLCD4 upregulates the Erk signaling pathway and promotes cell proliferation. by similar: Other phospholipases may also participate in similar PIP2 hydrolysis and signaling processes.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag GST
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Accession
Q9BRC7-1 (M1-S762)
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Gene ID84812
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Protein Length
Full Length of Isoform-1
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Synonyms
PLCD4; Phospholipase C, Delta 4; Phospholipase C Delta 4; Phospholipase C-Delta-4; 1-Phosphatidylinositol 4,5-Bisphosphate Phosphodiesterase Delta-4; PLC-Delta-4; Phosphoinositide Phospholipase C-Delta-4; PLC Delta4; Phosphoinositide Phospholipase C; HPLC
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AA Sequence
MASLLQDQLTTDQDLLLMQEGMPMRKVRSKSWKKLRYFRLQNDGMTVWHARQARGSAKPSFSISDVETIRNGHDSELLRSLAEELPLEQGFTIVFHGRRSNLDLMANSVEEAQIWMRGLQLLVDLVTSMDHQERLDQWLSDWFQRGDKNQDGKMSFQEVQRLLHLMNVEMDQEYAFSLFQAADTSQSGTLEGEEFVQFYKALTKRAEVQELFESFSADGQKLTLLEFLDFLQEEQKERDCTSELALELIDRYEPSDSGKLRHVLSMDGFLSYLCSKDGDIFNPACLPIYQDMTQPLNHYFICSSHNTYLVGDQLCGQSSVEGYIRALKRGCRCVEVDVWDGPSGEPVVYHGHTLTSRILFKDVVATVAQYAFQTSDYPVILSLETHCSWEQQQTMARHLTEILGEQLLSTTLDGVLPTQLPSPEELRRKILVKGKKLTLEEDLEYEEEEAEPELEESELALESQFETEPEPQEQNLQNKDKKKKSKPILCPALSSLVIYLKSVSFRSFTHSKEHYHFYEISSFSETKAKRLIKEAGNEFVQHNTWQLSRVYPSGLRTDSSNYNPQELWNAGCQMVAMNMQTAGLEMDICDGHFRQNGGCGYVLKPDFLRDIQSSFHPEKPISPFKAQTLLIQVISGQQLPKVDKTKEGSIVDPLVKVQIFGVRLDTARQETNYVENNGFNPYWGQTLCFRVLVPELAMLRFVVMDYDWKSRNDFIGQYTLPWTCMQQGYRHIHLLSKDGISLRPASIFVYICIQEGLEGDES
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Predicted Molecular Mass
110 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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