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PLVAP Protein, Human (HEK293, His)

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Based on 1 publication(s) in Google Scholar

The PLVAP protein is an endothelial cell-specific membrane protein that plays a key role in the complex structure of endothelial fenestrae by participating in the formation of septa that bridge these fenestrae. Furthermore, it plays a crucial role in the development of stomata within caveolae and transendothelial channels. PLVAP Protein, Human (HEK293, His) is the recombinant human-derived PLVAP protein, expressed by HEK293 , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The PLVAP protein is an endothelial cell-specific membrane protein that plays a key role in the complex structure of endothelial fenestrae by participating in the formation of septa that bridge these fenestrae. Furthermore, it plays a crucial role in the development of stomata within caveolae and transendothelial channels. PLVAP Protein, Human (HEK293, His) is the recombinant human-derived PLVAP protein, expressed by HEK293 , with N-His labeled tag.

Background

Plasmalemma Vesicle-Associated Protein (PLVAP) is a membrane protein exclusive to endothelial cells and is integral to the creation of diaphragms that bridge endothelial fenestrae. It plays a crucial role in the formation of stomata within caveolae and transendothelial channels. PLVAP is essential for regulating microvascular permeability, with endothelial fenestrae facilitating the controlled passage of water and solutes, thereby modulating transcellular versus paracellular flow in various organs. This protein's significance extends to embryonic development, where it performs a specific role. Structurally, PLVAP exists as a homodimer, emphasizing its importance in orchestrating complex endothelial functions (

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-8*His
  • Accession

    Q9BX97 (Y49-G442)

  • Gene ID
    83483  [NCBI]
  • Molecular Construction
    • N-term
    • 8*His
    • PLVAP (Y49-G442)
      Accession # Q9BX97
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    PLVAP; Fenestrated-Endothelial Linked Structure Protein; PV-1 Protein; Plasmalemma Vesicle Associated Protein; Fenestrated Endothelial-Linked Structure Protein; PV-1; Plasmalemma Vesicle-Associated Protein; FELS; Plasmalemma Vesicle Protein 1; PV1; DIAR10

  • AA Sequence

    YGNVHVSTESNLQATERRAEGLYSQLLGLTASQSNLTKELNFTTRAKDAIMQMWLNARRDLDRINASFRQCQGDRVIYTNNQRYMAAIILSEKQCRDQFKDMNKSCDALLFMLNQKVKTLEVEIAKEKTICTKDKESVLLNKRVAEEQLVECVKTRELQHQERQLAKEQLQKVQALCLPLDKDKFEMDLRNLWRDSIIPRSLDNLGYNLYHPLGSELASIRRACDHMPSLMSSKVEELARSLRADIERVARENSDLQRQKLEAQQGLRASQEAKQKVEKEAQAREAKLQAECSRQTQLALEEKAVLRKERDNLAKELEEKKREAEQLRMELAIRNSALDTCIKTKSQPMMPVSRPMGPVPNPQPIDPASLEEFKRKILESQRPPAGIPVAPSSG

  • Predicted Molecular Mass

    46 kDa

  • Molecular Weight

    Approximately 50-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, 200 mM L-arginine, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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