TCF4 Protein, Human (His, Strep)
Based on 1 Customer Validation
TCF4 Protein, Human (His, Strep) is the recombinant human-derived TCF4, expressed by E. coli , with Strep, His labeled tag. ,
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
TCF4 Protein, Human (His, Strep) is the recombinant human-derived TCF4, expressed by E. coli , with Strep, His labeled tag. ,
TCF7L2/TCF4 is a transcription factor involved in the Wnt signaling pathway, modulating MYC expression by binding to its promoter in a sequence-specific manner. It acts as a repressor in the absence of CTNNB1 and as an activator in its presence. In the presence of CTNNB1, TCF7L2/TCF4 activates transcription from promoters containing multiple copies of the Tcf motif 5'-CCTTTGATC-3'. TLE1, TLE2, TLE3, and TLE4 repress transactivation mediated by TCF7L2/TCF4 and CTNNB1. Expression of dominant-negative mutants results in cell-cycle arrest at the G1 phase. Additionally, TCF7L2/TCF4 is essential for maintaining the epithelial stem-cell compartment of the small intestine.
Technical Parameters
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Species Human
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Source E. coli
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Tag Strep;His
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Accession
Q9NQB0-1 (D10-T54)
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Gene ID6934
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Protein Length
Partial
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Synonyms
TCF7L2; TCF7L2 Isoform PFC8A_TCF7L2_A3,Ex1-12,13,13a; Prev. TCF4; Transcription Factor 7 Like 2 Isoform E1; TCF-4; Transcription Factor 7 Like 2 Isoform E4; Transcription Factor 7-Like 2; TCF7L2 Isoform PFC8A_TCF7L2_ex1-11-13-14; Transcription Factor 7-Li
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AA Sequence
DDLGANDELISFKDEGEQEEKSSENSSAERDLADVKSSLVNESET
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Predicted Molecular Mass
8.2 kDa
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Molecular Weight
Approximately 18 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200mM NaCl, 20% glycerol, 1mM DTT.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (232 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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