USP16 Protein, Human (sf9)

The USP16 protein is a specific deubiquitinase that targets "Lys-120" of histone H2A (H2AK119Ub), a tag associated with epigenetic transcriptional repression. By catalytically removing this ubiquitin moiety, USP16 acts as a coactivator, promoting subsequent phosphorylation of histone H3 (H3S10ph) 'Ser-11' to achieve mitotic chromosome segregation. USP16 Protein, Human (sf9) is the recombinant human-derived USP16 protein, expressed by sf9 insect cells , with tag free.

USP16 protein serves as a specific deubiquitinase, targeting 'Lys-120' of histone H2A (H2AK119Ub), a distinctive tag associated with epigenetic transcriptional repression. By catalyzing the removal of this ubiquitin moiety, USP16 acts as a coactivator, facilitating subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), a crucial event for chromosome segregation during mitosis. This deubiquitination process is indispensable for maintaining transcription in resting B- and T-lymphocytes, where phosphorylation by AURKB enhances USP16 activity. Furthermore, USP16 plays a regulatory role in Hox gene expression through histone H2A deubiquitination, with a preference for nucleosomal substrates. Notably, USP16 exhibits selectivity, as it does not deubiquitinate histone H2B. Beyond histones, USP16 extends its deubiquitinating activity to non-histone proteins, exemplified by its interaction with ribosomal protein RPS27A. In this context, USP16-mediated deubiquitination of monoubiquitinated RPS27A promotes the maturation of the 40S ribosomal subunit. These diverse functions highlight the multifaceted role of USP16 in the regulation of chromatin dynamics and cellular processes beyond histone modification.

The fundamental role of USP16 is specific removal of ubiquitin from substrates. USP16 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535nM emission light under the excitation condition of 485nM. The signal of which can be quickly and reliably captured using a microplate reader. The concentration of USP16 used is 0.5 nM, and the reaction lasts for 30 minutes. Under these conditions, the signal-to-noise ratio (S/N) of the signal box is greater than or equal to 10.

Human

Sf9 insect cells

Tag Free

Q9Y5T5 (G2-L823)

10600

Partial

GKKRTKGKTVPIDDSSETLEPVCRHIRKGLEQGNLKKALVNVEWNICQDCKTDNKVKDKAEEETEEKPSVWLCLKCGHQGCGRNSQEQHALKHYLTPRSEPHCLVLSLDNWSVWCYVCDNEVQYCSSNQLGQVVDYVRKQASITTPKPAEKDNGNIELENKKLEKESKNEQEREKKENMAKENPPMNSPCQITVKGLSNLGNTCFFNAVMQNLSQTPVLRELLKEVKMSGTIVKIEPPDLALTEPLEINLEPPGPLTLAMSQFLNEMQETKKGVVTPKELFSQVCKKAVRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFGNSTEKLDEELKNKVKDYEKKKSMPSFVDRIFGGELTSMIMCDQCRTVSLVHESFLDLSLPVLDDQSGKKSVNDKNLKKTVEDEDQDSEEEKDNDSYIKERSDIPSGTSKHLQKKAKKQAKKQAKNQRRQQKIQGKVLHLNDICTIDHPEDSEYEAEMSLQGEVNIKSNHISQEGVMHKEYCVNQKDLNGQAKMIESVTDNQKSTEEVDMKNINMDNDLEVLTSSPTRNLNGAYLTEGSNGEVDISNGFKNLNLNAALHPDEINIEILNDSHTPGTKVYEVVNEDPETAFCTLANREVFNTDECSIQHCLYQFTRNEKLRDANKLLCEVCTRRQCNGPKANIKGERKHVYTNAKKQMLISLAPPVLTLHLKRFQQAGFNLRKVNKHIKFPEILDLAPFCTLKCKNVAEENTRVLYSLYGVVEHSGTMRSGHYTAYAKARTANSHLSNLVLHGDIPQDFEMESKGQWFHISDTHVQAVPTTKVLNSQAYLLFYERIL

Approximately 100 kDa, based on SDS-PAGE under reducing conditions.

≥ 80%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5% glycerol, 2 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.