USP27X Protein, Human (His-Strep II)
USP27X Protein, Human (His-Strep II) is the recombinant human-derived USP27X, expressed by E. coli , with His-Strep II labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
USP27X Protein, Human (His-Strep II) is the recombinant human-derived USP27X, expressed by E. coli , with His-Strep II labeled tag.
Background
USP27X is a deubiquitinase involved in innate antiviral immunity, primarily by mediating the deubiquitination of CGAS and RIGI. It negatively regulates RIGI by catalyzing 'Lys-63'-linked deubiquitination of RIGI, thereby inhibiting type I interferon signaling. Additionally, USP27X regulates the 'Lys-63'-linked ubiquitination level of MDA5/IFIH1. As a positive regulator of the cGAS-STING pathway, it promotes the stabilization of CGAS by catalyzing its 'Lys-48'-linked deubiquitination. By similarity, USP27X can reduce BCL2L11/BIM ubiquitination levels and stabilize BCL2L11 in response to the RAF-MAPK-degradation signal, potentially counteracting the anti-apoptotic effects of MAPK activity by modulating BCL2L11 levels.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-His;N-StrepⅡ
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Accession
A6NNY8 (C2-Y438)
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Gene ID389856
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Molecular Construction
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N-term
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His-StrepⅡ
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USP27X (C2-Y438)
Accession # A6NNY8 -
6*His
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C-term
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Protein Length
Full Length
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Synonyms
USP27X; Ubiquitin Specific Peptidase 27 X-Linked; USP27; X-Linked Ubiquitin Carboxyl-Terminal Hydrolase 27; Ubiquitin Specific Protease 27, X Chromosome; Ubiquitin-Specific-Processing Protease 27; Ubiquitin Carboxyl-Terminal Hydrolase 27; Deubiquitinating
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AA Sequence
CKDYVYDKDIEQIAKEEQGEALKLQASTSTEVSHQQCSVPGLGEKFPTWETTKPELELLGHNPRRRRITSSFTIGLRGLINLGNTCFMNCIVQALTHTPILRDFFLSDRHRCEMPSPELCLVCEMSSLFRELYSGNPSPHVPYKLLHLVWIHARHLAGYRQQDAHEFLIAALDVLHRHCKGDDVGKAANNPNHCNCIIDQIFTGGLQSDVTCQACHGVSTTIDPCWDISLDLPGSCTSFWPMSPGRESSVNGESHIPGITTLTDCLRRFTRPEHLGSSAKIKCGSCQSYQESTKQLTMNKLPVVACFHFKRFEHSAKQRRKITTYISFPLELDMTPFMASSKESRMNGQLQLPTNSGNNENKYSLFAVVNHQGTLESGHYTSFIRHHKDQWFKCDDAVITKASIKDVLDSEGYLLFYHKQVLEHESEKVKEMNTQAY
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Predicted Molecular Mass
52.7 KDa
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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