Cat. No.: HY-N2620

Rutaevin

Customer Review

Based on 1 Customer Validation

Rutaevin is an antifungal toxin and CYP3A4 inactivator. Rutaevin requires NADPH-dependent bioactivation to generate cis-but-2-ene-1,4-dial, which then covalently modifies and mechanism-based inactivates human CYP3A4 (IC₅₀=4.48 μM, K_i=15.98 μM), and interacts with substances such as glutathione. Rutaevin disrupts the cellular structure of Sirococcus conigenus, accumulates linearly in resistant larch after pathogen exposure, and induces liver toxicity in mice via oral administration. Rutaevin can be applied to research in fields related to larch shoot blight and other associated areas.

For research use only. We do not sell to patients.

Purity: 99.77%
CAS No.: 33237-37-5
Formula: C₂₆H₃₀O₉
Molecular Weight:486.51

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Rutaevin Chemical Structure

CAS No.: 33237-37-5

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

CYP3A4

4.48 μM (IC₅₀)

All Cytochrome P450 Isoforms :

In Vitro

Rutaevin (20-100 μM; 1-2 h) undergoes NADPH-dependent bioactivation to form a reactive BDA intermediate in rat and human liver microsomes, which reacts with nucleophilic trapping agents GSH, NAL, and MOA to form 10 distinct conjugates, including the cyclic mono(GSH) conjugate M1^[1].
Rutaevin (0.1-30 μM; 30 min) acts as a potential mechanism-based inactivator of CYP3A4 in human liver microsomes, as shown by the 5.03-fold decrease in IC₅₀ (to 4.48 μM) following NADPH-dependent preincubation^[1].
Rutaevin (0.0625-1.0000 mg/mL; up to 5 days) potently inhibits the growth of Neofusicoccum laricinum with an IC₅₀ of 0.27 mg/mL and achieves complete inhibition at 0.5 mg/mL^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Rutaevin (5 mg/kg; i.v.; single dose) undergoes in vivo bioactivation in male Sprague-Dawley rats, producing a cyclic mono(GSH) conjugate (M1) of its reactive BDA intermediate^[1].
Rutaevin (oral) induces hepatotoxicity in mice, as indicated by elevated serum ALT and AST activities^[1].
Rutaevin (p.o.) induces hepatotoxicity in mice via bioactivation processes^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Sprague-Dawley (male, 220-250 g, bile duct cannulated)^[1]
Dosage:

5 mg/kg
Administration:

i.v.; single dose
Result:

Detected a cyclic mono(GSH) conjugate of rutaevin's bioactivated cis-butene-1,4-dial (BDA) intermediate (designated M1) as the most abundant metabolite in rat bile samples.

Chemical Information

CAS No. 33237-37-5
Appearance Solid
Molecular Weight 486.51
Formula C₂₆H₃₀O₉
Color White to off-white
SMILES

C[C@]1([C@H]2O)[C@@]3([C@@H]4O3)[C@]([C@H](C5=COC=C5)OC4=O)(CC[C@]1([H])[C@]67[C@](C(C)(C)O[C@@]6([H])CC(OC7)=O)([H])C2=O)C
Structure Classification
- Terpenoids
- Other Terpenoids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Liu Y, et al. Cytochrome P450 mediated bioactivation of rutaevin, a bioactive and potentially hepatotoxic component of Evodia rutaecarpa[J]. Chemical Research in Toxicology, 2020, 33(12): 3054-3064.

[2]. Zhang R, et al. From Resistance Mechanism to Green Application: Discovery of Rutaevin as a Key Phytoalexin in Larch and Cross-Species Resource Optimization. Plants (Basel). 2025;14(19):2947. Published 2025 Sep 23. [Content Brief]

Calculators

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Rutaevin

Bioactive Compound Library Max

Bioactive Compound Library

Anti-Infection Compound Library

Metabolism/Protease Compound Library

Natural Product Library

Anti-Aging Compound Library

Covalent Screening Library

Antioxidant Compound Library

Oxygen Sensing Compound Library

Medicine Food Homology Compound Library

Terpenoids Library

Traditional Chinese Medicine Active Compound Library

Mitochondria-Targeted Compound Library

Plant-Sourced Natural Product Library

Cuproptosis Compound Library

Heterocyclic Compound Library

Metabolic Enzyme Compound Library

Cysteine Targeted Covalent Library

Anti-Drug-Resistant Compound Library

Radioprotector Library

MCE Bioactive Compound Library

Covalent Screening Library Plus

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Miao Ethnicity Medicine Compound Library

Tibetan Medicine Compound Library

PBCRBS Traditional Chinese Medicine Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Natural Product Diversity Scaffold Library

Mass Spectrometry Natural Product Library

All Cytochrome P450 Isoforms

Biological Activity

Chemical Information

Purity & Documentation

References

Calculators

Help & FAQs

Rutaevin Related Classifications

HY-N2620 Related Classifications