Search Result
Results for "
Colorimetric
" in MedChemExpress (MCE) Product Catalog:
16
Biochemical Assay Reagents
2
Isotope-Labeled Compounds
| Cat. No. |
Product Name |
Target |
Research Areas |
Chemical Structure |
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- HY-15924
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Thiazolyl Blue
Maximum Cited Publications
182 Publications Verification
MTT; Thiazolyl Blue Tetrazolium bromide; Methylthiazolyldiphenyl-tetrazolium bromide
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Fluorescent Dye
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Others
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Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis .
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- HY-137805
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Fluorescent Dye
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Others
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Ferrozine is a spectrophotometric reagent for iron ions, can react with divalent Fe to form a stable magenta complex species. The complex has an absorption peak at 562 nm . Ferrozine-based colorimetric assays can quantify iron in cells
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- HY-W115718
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Dopamine β-hydroxylase
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Neurological Disease
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Cuprizone is a copper chelating agent that forms a deep blue copper ketone complex with copper (II). The copper ketone reaction can be used in colorimetric tests for the presence of trace copper. Cuprizone can be used to induce some schizophrenia-like behavior in mice. Cuprizone acts on copper enzymes, including SOD1, cytochrome oxidase, and DβH, thereby causing oxidative stress and increasing DA levels in certain brain regions such as the medial prefrontal cortex (PFC) .
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- HY-W034953
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Biochemical Assay Reagents
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Others
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Bathocuproine disulfonate disodium (BCS) is an organic compound used as a highly sensitive colorimetric reagent for copper ions in biochemical and analytical applications. It has a bright yellow color and absorbs light at specific wavelengths, so it can be used to detect and quantify trace copper. In biochemical applications, BCS is commonly used to study the role of copper ions in various biological processes. Copper is an essential nutrient for many organisms, but it can also be toxic in high concentrations, so accurate measurement of copper levels is important to understand its impact on living systems. In terms of analysis, BCS is often used in environmental monitoring and water quality testing to detect copper pollution. It can detect copper even at very low concentrations, making it an invaluable tool for identifying potential sources of pollution and assessing the impact of industrial activities on aquatic ecosystems.
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- HY-W009694
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Biochemical Assay Reagents
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Others
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3,5-Dinitrosalicylic acid the derivative of salicylic acid. 3,5-Dinitrosalicylic acid is used in the α-amylase assay, carbohydrase assay, and for the colorimetric determination of reducing substances .
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- HY-15926
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ONPG
Maximum Cited Publications
6 Publications Verification
2-Nitrophenyl β-D-galactopyranoside
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Biochemical Assay Reagents
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Others
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ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity.
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- HY-15920
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INT
3 Publications Verification
Iodonitrotetrazolium chloride; p-Iodonitrotetrazolium Violet
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Biochemical Assay Reagents
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Others
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INT (Iodonitrotetrazolium chloride) is a biochemical reagent mainly used in the iodonitrotetrazolium chloride (INT) colorimetric method to assess the metabolic activity of cells and the activity of enzymes .
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- HY-77962
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Biochemical Assay Reagents
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Others
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2-Thiobarbituric acid is a commonly used colorimetric reagent for the detection of malondialdehyde (MDA), a marker of lipid peroxidation. 2-Thiobarbituric acid forms a complex with MDA that can be quantified by colorimetric detection at 532 nm as a measure of lipid peroxidation .
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- HY-D1224
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CPRG
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Biochemical Assay Reagents
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Others
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Chlorophenol red-β-D-galactopyranoside (CPRG) is an efficient and sensitive chromogenic substrate for β-galactosidase (HY-P2869), widely used in colorimetric assays. Chlorophenol red-β-D-galactopyranoside itself appears pale yellow. When it is specifically hydrolyzed by β-galactosidase, it releases chlorophenol red. The released chlorophenol red turns purple-red under alkaline or neutral pH conditions. This color change from yellow to red can be quantitatively detected at wavelengths of 540-572 nm using visible spectrophotometry .
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- HY-B1306
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p-Aminohippuric acid
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Others
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Neurological Disease
Metabolic Disease
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4-Aminohippuric acid (p-Aminohippuric acid) is a coordination ligand for metal ions (such as Cu 2+, Fe 3+, Hg 2+) and a functionalization reagent for nanomaterials. 4-Aminohippuric acid can coordinate with metal ions or modify the surface of materials such as carbon nanotubes and gold nanoparticles through amino and carboxyl groups. 4-Aminohippuric acid can form stable complexes with metal ions or participate in the synthesis of nanomaterials as a reducing agent/stabilizer, enriching metal ions or giving nanoparticles peroxidase-mimicking activity. 4-Aminohippuric acid can be used to construct highly sensitive electrochemical sensors or colorimetric sensors to detect and quantitatively analyze heavy metal ions such as copper, iron, and mercury in environmental water samples and biological samples. 4-Aminohippuric acid may also be a biomarker for attention-deficit/hyperactivity disorder (ADHD) .
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- HY-15909
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BCIP p-toluidine salt; X-phosphate p-toluidine salt
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Fluorescent Dye
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Others
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BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity .
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- HY-W013168
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4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate; pNpp
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Lipase
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Others
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4-Nitrophenyl palmitate (4-Nitrophenyl hexadecanoate) is a chromogenic substrate for lipases and esterases. Upon enzymatic hydrolysis, 4-Nitrophenyl palmitate releases p-nitrophenol, which can be quantified by colorimetric detection at 410 nm as a measure of enzymatic activity. 4-Nitrophenyl palmitate is used to characterize the activity of various bacterial and mammalian enzymes, including those from Burkholderia and porcine pancreatic lipase .
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- HY-W129633
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DCIP sodium hydrate; DPIP sodium hydrate
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Biochemical Assay Reagents
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Others
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2,6-Dichlorophenolindophenol (DCIP; DPIP) sodium hydrate is a redox chromogenic indicator with a redox potential of +217 mV relative to SHE, and it acts as a substrate for reduction reactions. During the metabolic process of nutrient consumption by Saccharomyces cerevisiae, 2,6-Dichlorophenolindophenol sodium hydrate is reduced from dark blue to colorless, resulting in a decrease in absorbance. 2,6-Dichlorophenolindophenol sodium hydrate is widely used in spectrophotometric biochemical oxygen demand determination and preclinical colorimetric toxicity analysis for heavy metal ion detection based on Saccharomyces cerevisiae .
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- HY-D0221
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NSC 112125; 2,4,6-Tipyidyl-s-tiazie
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MOFs
Fluorescent Dye
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Others
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2,4,6-Tri-2-pyridinyl-1,3,5-triazine (NSC 112125) is a colorimetric reagent for detecting iron. 2,4,6-Tri-2-pyridinyl-1,3,5-triazine forms a complex with Fe (II) and can be quantified as a measure of iron concentration by colorimetric detection at 594 nm .
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- HY-125815
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Fluorescent Dye
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Others
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Reactive Blue 4 is an anthraquinone dye used extensively in the textile industry. Reactive Blue 4 is a single colorimetric chemosensor for sequential determination of multiple analytes with different optical responses in aqueous media .
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- HY-151644
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Fluorescent Dye
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Others
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Fluorescein hydrazide is a sensitive fluorescent probe for Hg 2+ and Co 2+ that induces obvious color changes and fluorescence changes. Fluorescein hydrazide can be prepared by the reaction of fluorescein and hydrazine. Fluorescein hydrazide exhibits an excitation wavelength of 508 nM and an emission wavelength of 531 nM [2][3].
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- HY-131409
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D-Val-Leu-Lys-p-itoailide dihydrochloride; D-VLK-pNA dihydrochloride; S 2251 dihydrochloride
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Fluorescent Dye
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Others
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D-Val-Leu-Lys-pNA (D-Val-Leu-Lys-p-nitroanilide) dihydrochloride is a chromogenic peptide substrate that serves as a characteristic substrate for plasmin and plasminogen. D-Val-Leu-Lys-pNA dihydrochloride acts as a sensitive substrate for the DFE27 serine protease derived from Bacillus subtilis DC27. Catalyzed by plasmin, D-Val-Leu-Lys-pNA dihydrochloride binds and hydrolyzes to release p-nitroaniline (pNA), which can be detected colorimetrically at 405 nm as a measure of fibrinolytic activity .
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- HY-P3236
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Ac-IEPD-pNA
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Ser/Thr Protease
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Cancer
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N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide (Ac-IEPD-pNA) is a colorimetric peptide substrate of granzyme B that allows accurate measurement of granzyme B activity .
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- HY-W115738
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Biochemical Assay Reagents
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Others
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PAR is an azo dye widely used as a colorimetric reagent for metal ions. PAR forms stable chelates with different metal ions. PAR can also complex with heavy metal ions in polar organic solvents, such as ethanol .
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- HY-Y0699
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Environmental Pollutants
Fluorescent Dye
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Others
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Methyl red is a pH-sensitive acid-base indicator and colorimetric reagent with a pKa of 5.1. Methyl red achieves visual judgment of the titration endpoint through reversible structural changes of protonation (red, pH <4.4) and deprotonation (yellow, pH >6.2). Methyl red is widely used in acid-base titration in chemical analysis and buffer pH monitoring in biochemistry .
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- HY-D0202
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Fluorescent Dye
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Others
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Arsenazo III is an azo derivative of chromotropic acid, a metal chrome dye, a chelating agent, and a cation complexing agent. Arsenazo III forms stable 1:1 complexes with Ca 2+, Mg 2+, Sr 2+, Ba 2+, K +, and Na +, and its binding affinity for Ca 2+ depends on pH, alkali metal cation concentration, and buffer parameters. Arsenazo III serves as a colorimetric indicator for micromolar ionized Ca 2+ in cells .
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- HY-W011654
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4-Aminophenyl-beta-D-galactopyranoside, 98%
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Glycosidase
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Inflammation/Immunology
Cancer
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4-Aminophenyl-β-D-galactopyranoside, 98% is a highly efficient substrate for β-galactosidase. It is specifically hydrolyzed by this enzyme to release galactose and electroactive p-aminophenol. 4-Aminophenyl-β-D-galactopyranoside, 98% is widely used in colorimetric and electrochemical assays for detecting β-galactosidase activity and determining enzyme kinetics, such as in biosensing fields including cellular senescence, pathogen and contaminant detection. In addition, since β-galactosidase is often overexpressed in primary ovarian cancer, 4-Aminophenyl-β-D-galactopyranoside, 98% can also be applied to related research on primary ovarian cancer .
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- HY-15929
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TBHBA
1 Publications Verification
2,4,6-Tribromo-3-hydroxybenzoic acid
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Biochemical Assay Reagents
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Others
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TBHBA (2,4,6-Tribromo-3-hydroxybenzoic acid) is a chemical colorimetric agent and nanomaterial surface modifier. TBHBA can react with some oxidants (such as H2O2) to generate high-absorbance quinoneimine dyes, which enhance the sensitivity of HDL cholesterol enzymatic determination. TBHBA can be used as a bromine-containing coupling agent to modify the surface of silica nanoparticles through esterification reaction. Thereby, organic flame retardant groups are introduced to improve the thermal stability and flame retardant properties of nanocomposites. TBHBA can be used in clinical biochemical testing (such as serum lipoprotein analysis) and the preparation of flame-retardant polymer nanocomposites .
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- HY-D1599
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R6GH
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Fluorescent Dye
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Others
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Rhodamine 6G hydrazide (R6GH) is a fluorescent dye. Rhodamine 6G hydrazide can be used in selective colorimetric and fluorescent sensing .
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- HY-D0943
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Kernechtrot
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Fluorescent Dye
Photosensitizer
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Others
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Nuclear Fast Red, an anthraquinone dye, is commonly used in conjunction with an excess of aluminum ions as a red nuclear counterstain. Nuclear fast red has been used as a histochemical and colorimetric reagent for calcium . Nuclear fast red as highly sensitive "off/on" fluorescent probe for detecting guanine .
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- HY-W011911
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- HY-W110793
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Diphenylthiocarbazone
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Biochemical Assay Reagents
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Metabolic Disease
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Dithizone can be toxic to pancreatic islet cells and cause experimental diabetes. Dithizone can be used for in vitro in vivo staining of transplanted islets and cytoplasmic granules of bone marrow cells. Dithizone can be used as a chelating agent and colorimetric indicator for the detection and extraction of various heavy metals, including lead and mercury, in analytical and biochemical applications. Dithizone forms stable colored complexes with metal ions and can be quantitatively analyzed by methods such as spectrophotometry .
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- HY-D2329
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Fluorescent Dye
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Others
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Fluorescein-PEG4-NHS ester is a colorimetric probe formed by the reaction of adipic dihydrazide and fluorescein isothiocyanate .
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- HY-D0813
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Fluorescent Dye
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Others
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Zincon sodium is an excellent colorimetric reagent for the detection of zinc and copper ions in aqueous solution. Zincon sodium can be used for the determination of zinc, copper, and cobalt ions in metalloproteins .
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- HY-W009454
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4-Nitopheyl Sulfate potassium
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Fluorescent Dye
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Others
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Potassium p-nitrophenyl sulfate (4-Nitophenyl Sulfate potassium) serves as a chromogenic substrate for arylsulfatases. Potassium p-nitrophenyl sulfate releases nitrophenol under the cleavage action of arylsulfatases, and the activity of arylsulfatases can be quantitatively determined via colorimetric detection at 400 nm. Potassium p-nitrophenyl sulfate is commonly used in enzyme inhibition assays and metabolite analog-related studies .
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- HY-W127790
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Biochemical Assay Reagents
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Others
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Bathophenanthrolinedisulfonic acid disodium salt (BPS) is a colorimetric reagent for iron detection, with a wavelength of 535 nm for iron complex. It can be used for iron determination in aqueous solution systems without the need for extraction solvents. BPS can serve as a precursor to prepare Europium(III) tris(dibenzoylmethanate) bis(bathophenanthrolinedisulfonate) complex and ruthenium(II) tris(bathophenanthrolinedisulfonate), or participate in the catalytic oxidation of 2-hexanol by forming water-soluble complexes with palladium .
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- HY-107864
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Tetraiodofluorescein
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Fluorescent Dye
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Infection
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Erythrosine B free acid is a visibly red dye with colorimetric and fluorescent properties that serves as an important dye for many Gram-positive and -negative bacteria. Erythrosine B free acid can be used for live/dead determination in both colorimetric and fluorescence-based assays for low, medium and high-throughput experimentation .
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- HY-121694
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Beta-lactamase
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Infection
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CENTA is a colorimetric cephalosporin substrate for β-lactamases. Upon hydrolysis by β-lacatamases, CENTA turns from light yellow to chrome yellow, which can be quantified by colorimetric detection at 405 nm as a measure of β-lactamase activity.
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- HY-D0108
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Fluorescent Dye
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Fluorescein dilaurate is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.
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- HY-134354A
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ADP-ribose-pNP disodium
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Poly(ADP-ribose) Glycohydrolase (PARG)
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Others
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pNP-ADPr disodium is a colorimetric substrate that used for the first continuous Poly(ADP-ribose) glycohydrolase (PARG) and ADP-ribosyl hydrolase 3 (ARH3) activity assays. pNP-ADPr disodium can be used for the research of poly(ADP-ribose)polymerase (PARP) enzymes .
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- HY-120833
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Caspase
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Others
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Ac-Ile-Glu-Thr-Asp-pNA is a substrate for caspase-8. Caspase-8 binds to and cleaves the Ile-Glu-Thr-Asp (IETD) peptide sequence to release p-nitroalinide, which can be quantified by colorimetric detection at 405 nm as a measure of enzyme activity .
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- HY-W009311
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4,4'-Bis(dimethylamino)thiobenzophenone
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Biochemical Assay Reagents
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Others
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Thiomichler's ketone (4,4'-Bis (dimethylamino) thiobenzophenone) is a heavy metal complexing agent and a colorimetric/spectrophotometric reagent. Thiomichler's ketone shows selectivity for Hg 2+ at pH 3, and exhibits activity towards Pd 2+ and Ag + at pH 3.5. Thiomichler's ketone enables accurate detection of trace Pd 2+ by forming an extractable Hg 2+ complex via micelle-mediated cloud point extraction, or generating a red coordination complex including Pd (TMK)4. Thiomichler's ketone is applicable for the determination of trace Pd 2+ in antibiotics and catalysts of automobile exhaust purifiers .
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- HY-B1306R
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p-Aminohippuric acid (Standard)
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Reference Standards
Biochemical Assay Reagents
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Neurological Disease
Metabolic Disease
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4-Aminohippuric acid (p-Aminohippuric acid) (Standard) is the analytical standard of 4-Aminohippuric acid (HY-B1306). This product is intended for research and analytical applications. 4-Aminohippuric acid (p-Aminohippuric acid) is a coordination ligand for metal ions (such as Cu 2+, Fe 3+, Hg 2+) and a functionalization reagent for nanomaterials. 4-Aminohippuric acid can coordinate with metal ions or modify the surface of materials such as carbon nanotubes and gold nanoparticles through amino and carboxyl groups. 4-Aminohippuric acid can form stable complexes with metal ions or participate in the synthesis of nanomaterials as a reducing agent/stabilizer, enriching metal ions or giving nanoparticles peroxidase-mimicking activity. 4-Aminohippuric acid can be used to construct highly sensitive electrochemical sensors or colorimetric sensors to detect and quantitatively analyze heavy metal ions such as copper, iron, and mercury in environmental water samples and biological samples. 4-Aminohippuric acid may also be a biomarker for attention-deficit/hyperactivity disorder (ADHD) .
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- HY-101263
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- HY-121549
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4-Nitrophenyl-2S,3S-epoxy-3 phenylpropyl carbonate
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Epoxide Hydrolase
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Others
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S-NEPC (4-Nitrophenyl-2S,3S-epoxy-3 phenylpropyl carbonate) is a colorimetric substrate used to measure epoxide hydrolase activity .
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- HY-134354
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ADP-ribose-pNP
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Poly(ADP-ribose) Glycohydrolase (PARG)
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Others
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pNP-ADPr is a colorimetric substrate that used for the first continuous Poly(ADP-ribose) glycohydrolase (PARG) and ADP-ribosyl hydrolase 3 (ARH3) activity assays. pNP-ADPr can be used for the research of poly(ADP-ribose)polymerase (PARP) enzymes .
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- HY-P10236
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Caspase
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Others
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Ac-WEHD-pNA is a colorimetric substrate for caspase 1, 4, 5 and 14, which is utilized to measure the caspase activity .
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- HY-15926S
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2-Nitrophenyl β-D-galactopyranoside-13C
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Isotope-Labeled Compounds
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Others
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ONPG- 13C is the 13C labeled ONPG. ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activit .
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- HY-138653
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DGGR; 1,2-o-Dilauryl-rac-glycero-3-glutaric acid (6′-methylresorufin) ester
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Lipase
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Metabolic Disease
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Lipase Substrate is a chromogenic substrate of lipase to detect activity . It is used in colorimetric methods to measure lipase activity. It produces a red-purple compound, methylresorufin, upon digestion by the lipase enzyme.
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- HY-D0374
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Fluorescent Dye
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Others
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Reactive Blue 4 sodium is an anthraquinone dye used extensively in the textile industry. Reactive Blue 4 sodium is a single colorimetric chemosensor for sequential determination of multiple analytes with different optical responses in aqueous media .
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- HY-W151629A
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Fluorescent Dye
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Others
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Ferrozine sodium hydrate is a spectrophotometric reagent for iron, can react with divalent Fe to form a stable magenta complex species. The complex has an absorption peak at 562 nm .Ferrozine sodium hydrate-based colorimetric assays can quantify iron in cells
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- HY-W115718R
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Reference Standards
Dopamine β-hydroxylase
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Neurological Disease
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Cuprizone is a copper chelating agent that forms a deep blue copper ketone complex with copper (II). The copper ketone reaction can be used in colorimetric tests for the presence of trace copper. Cuprizone can be used to induce some schizophrenia-like behavior in mice. Cuprizone acts on copper enzymes, including SOD1, cytochrome oxidase, and DβH, thereby causing oxidative stress and increasing DA levels in certain brain regions such as the medial prefrontal cortex (PFC) .
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- HY-W004617
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4,4',4''-(1,3,5-Triazine-2,4,6-triyl)tribenzoic acid
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MOFs
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Others
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2,4,6-Tris(4-carboxyphenyl)-1,3,5-triazine (4,4',4''-(1,3,5-Triazine-2,4,6-triyl) tribenzoic acid) is a metal-organic framework. 2,4,6-Tris(4-carboxyphenyl)-1,3,5-triazine is a C3-symmetric organic ligand with temperature-sensitive ratioluminescent properties. Complexes derived from 2,4,6-Tris(4-carboxyphenyl)-1,3,5-triazine exhibit strong emissivity and can be used for highly sensitive ratiometric fluorescent temperature sensing .
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- HY-B1306S
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p-Aminohippuric acid-d4
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Biochemical Assay Reagents
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Neurological Disease
Metabolic Disease
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4-Aminohippuric acid-d4 (p-Aminohippuric acid-d4) is the deuterium labeled 4-Aminohippuric acid (HY-B1306). 4-Aminohippuric acid (p-Aminohippuric acid) is a coordination ligand for metal ions (such as Cu 2+, Fe 3+, Hg 2+) and a functionalization reagent for nanomaterials. 4-Aminohippuric acid can coordinate with metal ions or modify the surface of materials such as carbon nanotubes and gold nanoparticles through amino and carboxyl groups. 4-Aminohippuric acid can form stable complexes with metal ions or participate in the synthesis of nanomaterials as a reducing agent/stabilizer, enriching metal ions or giving nanoparticles peroxidase-mimicking activity. 4-Aminohippuric acid can be used to construct highly sensitive electrochemical sensors or colorimetric sensors to detect and quantitatively analyze heavy metal ions such as copper, iron, and mercury in environmental water samples and biological samples. 4-Aminohippuric acid may also be a biomarker for attention-deficit/hyperactivity disorder (ADHD) .
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- HY-W142631
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Fluorescent Dye
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Cancer
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4-(Phenylazo)diphenylamine is an excellent colorimetric indicator for the accurate determination of the concentration for a variety of strong bases, Lewis acids, and hydride reducing agents .
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- HY-W106762
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3,4,9,10-Perylenetetracarboxylic acid
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Drug Intermediate
Biochemical Assay Reagents
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Others
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Perylene-3,4,9,10-tetracarboxylic acid (3,4,9,10-Perylenetetracarboxylic acid) is a chemical intermediate. Derivatives formed from Perylene-3,4,9,10-tetracarboxylic acid can serve as colorimetric and fluorescent chemosensors with selectivity for fluoride ions, and also act as charge generation materials in electrophotographic photoreceptors, organic electrophotographic photoreceptors, optoelectronic and photonic devices .
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- HY-137858
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Gly-Arg-4-NA hydrochloride; GR-pNA hydrochloride; GR p-itoailide hydrochloride
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Fluorescent Dye
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Others
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H-Gly-Arg-pNA hydrochloride (GR-pNA hydrochloride) is a colorimetric substrate for thrombin. H-Gly-Arg-pNA is preferentially bound by thrombin and cleaves the Gly-Arg (GR) peptide sequence, releasing p-nitroaniline (pNA) that can be detected colorimetrically at 405 nm. H-Gly-Arg-pNA hydrochloride is used as a fluorescent dye to measure thrombin activity .
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- HY-W015012
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Fluorescent Dye
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Others
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NBD-Hydrazine is an aldehyde- and ketone-reactive fluorescent probe. NBD-Hydrazine has excitation/emission maxima of 468/535 nm in the presence of aldehydes or ketones. NBD-Hydrazine can be used as a starting material to synthesize malondialdehyde-reactive fluorescent probes. NBD-Hydrazine is a synthetic intermediate in the synthesis of cupric and chronic ion colorimetric sensors .
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- HY-D1224A
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CPRG sodium
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Biochemical Assay Reagents
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Others
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Chlorophenol red-β-D-galactopyranoside (CPRG) sodium is an efficient and sensitive chromogenic substrate for β-galactosidase (HY-P2869), widely used in colorimetric assays. Chlorophenol red-β-D-galactopyranoside sodium itself appears pale yellow. When it is specifically hydrolyzed by β-galactosidase, it releases chlorophenol red. The released chlorophenol red turns purple-red under alkaline or neutral pH conditions. This color change from yellow to red can be quantitatively detected at wavelengths of 540-572 nm using visible spectrophotometry .
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- HY-126785
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Colorimetric Elastase Substrate
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Elastase
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Others
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Suc-AAP-Abu-pNA (Colorimetric Elastase Substrate) is a specific substrate for pancreatic elastase (Km = 100 μM; Kcat/Km = 35,300 s -1 M -1 for rat pancreatic elastase; Km = 30 μM; Kcat/Km = 351,000 s -1 M -1 for porcine pancreatic elastase). Suc-AAP-Abu-pNA also promotes OPC migration .
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- HY-158735
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Biochemical Assay Reagents
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Others
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Thioarginine hydrobromide is a colorimetric substrate for arginase that can be used in the spectrophotometric assay of arginase .
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- HY-136898
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Thrombin
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Others
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PS-915 dihydrochloride is a peptide substrate used in a colorimetric assay for plasma antithrombin III (ATIII). PS-915 dihydrochloride is highly specific for thrombin. By enzyme hydrolysis, PS-915 dihydrochloride liberates 3-carboxy-4-hydroxyaniline (CHA), which turns blue in color due to the complex formation with added alkaline-pentacyanoammine ferroate .
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-
- HY-D0107
-
|
|
Fluorescent Dye
|
|
|
Fluorescein dicaproate is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.
|
-
- HY-D0101
-
|
|
Fluorescent Dye
|
|
|
Fluorescein-diphosphat (diammonium) is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.
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-
- HY-D0105
-
|
|
Fluorescent Dye
|
|
|
Fluorescein diacetate 6-isothiocyanate is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.
|
-
- HY-165025
-
-
- HY-D0103
-
|
|
Fluorescent Dye
|
|
|
Fluorescein diacetate 5-maleimide is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.
|
-
- HY-D0100
-
|
|
Fluorescent Dye
|
|
|
Fluorescein-diacetate-5-isothiocyanat is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.
|
-
- HY-W011422
-
|
NSC 528
|
Fluorescent Dye
|
Others
|
|
2,6-Dibromo-4-(chloroimino)cyclohexa-2,5-dienone (NSC 528) is a colorimetric dye used for the detection of phenolic compounds. 2,6-Dibromo-4-(chloroimino)cyclohexa-2,5-dienone reacts with phenolic compounds at a pH of about 9.4 to produce an indigo dye. 2,6-Dibromo-4-(chloroimino)cyclohexa-2,5-dienone has been used as a Gibbs reagent for the colorimetric detection of phenolic compounds. 2,6-Dibromo-4-(chloroimino)cyclohexa-2,5-dienone has also been used in a colorimetric reaction for the quantitative colorimetric detection of aflatoxins, turning the colorless aflatoxins green with an absorption band at 673 nm.
|
-
- HY-P4335
-
|
|
Ser/Thr Protease
|
Others
|
|
Bz-RGFFP-4MβNA, a substrate for Cathepsin D, is suitable for either colorimetric or fluorometric assay .
|
-
- HY-W129500
-
|
|
Biochemical Assay Reagents
|
Others
|
|
N-[[Bis[4-(dimethylamino)phenyl]amino]carbonyl]glycine sodium can be used as a biomonitoring reagent to measure redox activity through a colorimetric reaction.
|
-
- HY-P10001
-
|
Z-Ile-Glu-Thr-Asp-pNA
|
Caspase
|
Cancer
|
|
Z-IETD-pNA (Z-Ile-Glu-Thr-Asp-pNA) is a colorimetric caspase-8 and granzyme B substrate. Z-IETD-pNA is hydrlyzed by caspase 8 to generate pNA .
|
-
- HY-W009458
-
|
3-IP sodium
|
Phosphatase
|
Metabolic Disease
|
|
3-Indoxyl phosphate (3-IP) is a chromogenic and electrochemical substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity.2 3-Indoxyl phosphate has been used in ELISAs.
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-
- HY-145499
-
|
|
Fluorescent Dye
|
Others
|
|
XTT is a cell-impermeable, negatively charged tetrazolium dye that produces a water-soluble formazan when reduced at the cell surface by cellular-derived NADH and an electron mediator. It is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis.
|
-
- HY-157926
-
|
|
Biochemical Assay Reagents
|
Others
|
|
Nitroso-PSAP is a sensitive and specific chromogenic reagent for iron (Fe 2+), which is used for the colorimetric determination of iron in blood after mineralization with periodic acid. TNitroso-PSAP chelates with iron (Fe 2+) to produce a green complex (λmax=756 nm, Epsilon = 4.5 x 10 < sup > 4 < / sup > dm < sup > 3 < / sup > mol < sup > 1 < / sup > cm < sup > 1 < / sup >) .
|
-
- HY-168378
-
|
S-2251
|
Fluorescent Dye
|
Others
|
|
D-Val-Leu-Lys-pNA (S-2251) is a chromogenic peptide substrate that serves as a characteristic substrate for plasmin and plasminogen. D-Val-Leu-Lys-pNA acts as a sensitive substrate for the DFE27 serine protease derived from Bacillus subtilis DC27. Catalyzed by plasmin, D-Val-Leu-Lys-pNA binds and hydrolyzes to release p-nitroaniline (pNA), which can be detected colorimetrically at 405 nm as a measure of fibrinolytic activity .
|
-
- HY-120971
-
|
DepNA
|
Endogenous Metabolite
|
Metabolic Disease
|
|
N-Decanoyl p-nitroaniline (DepNA) is one of several nitroaniline fatty acid amides which can be used to measure fatty acid amide hydrolase (FAAH) activity.1 FAAH is a relatively unselective enzyme in that it accepts a variety of amide head groups other than the ethanolamine of its endogenous substrate anandamide (AEA). It also will hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate. Exposure of DepNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε=13,500 at 410 nm). This allows the fast and convenient measurement of FAAH activity using a 96 well plate spectrophotometer.
|
-
- HY-107864A
-
|
Tetraiodofluorescein aluminum
|
Fluorescent Dye
|
Infection
|
|
Erythrosine B aluminum is a visibly red dye with colorimetric and fluorescent properties that serves as an important dye for many Gram-positive and -negative bacteria. Erythrosine B aluminum can be used for live/dead determination in both colorimetric and fluorescence-based assays for low, medium and high-throughput experimentation .
|
-
- HY-107864R
-
|
Tetraiodofluorescein (Standard)
|
Reference Standards
Fluorescent Dye
|
Infection
|
|
Erythrosine B free acid (Standard) is the analytical standard of Erythrosine B (free acid) (HY-107864). This product is intended for research and analytical applications. Erythrosine B free acid is a visibly red dye with colorimetric and fluorescent properties that serves as an important dye for many Gram-positive and -negative bacteria. Erythrosine B free acid can be used for live/dead determination in both colorimetric and fluorescence-based assays for low, medium and high-throughput experimentation .
|
-
- HY-D2969
-
|
|
Fluorescent Dye
|
Metabolic Disease
|
|
CYDA is a colorimetric probe based on cyanine dyes, which enables highly sensitive and selective detection of Cu²⁺. CYDA can be used for urine copper detection in Wilson's disease .
|
-
- HY-171819
-
|
|
|
Infection
|
|
O157 Medium powder can be used to prepare a colorimetric medium for the separation and differentiation of Escherichia coli O157.
|
-
- HY-DY1106
-
|
|
Fluorescent Dye
|
Others
|
Ferrozine (solution) is a spectrophotometric reagent for iron ions, can react with divalent Fe to form a stable magenta complex species. The complex has an absorption peak at 562 nm . Ferrozine-based colorimetric assays can quantify iron in cells Solvent and concentration: ddH2O: 10 mM
|
-
- HY-DY1086
-
|
MTT (solution); Thiazolyl Blue Tetrazolium bromide (solution); Methylthiazolyldiphenyl-tetrazolium bromide (solution)
|
Fluorescent Dye
|
Others
|
Thiazolyl Blue (solution) (MTT (solution)) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis . Solvent and concentration: PBS: 5 mg/mL
|
-
- HY-D3244
-
|
|
Fluorescent Dye
|
Others
|
|
RDDB is a rhodamine-based turn-on fluorescent and colorimetric chemosensor selective for Mn 2+, with a limit of detection of 5×10 -8 M (excitation wavelength: 480-550 nm; fluorescence signal collection range: >590 nm). In the presence of Mn 2+, RDDB undergoes a spirolactam ring-opening reaction of its rhodamine hydrazide moiety, resulting in turn-on fluorescence and a color change. RDDB can be used for intracellular Mn 2+ imaging .
|
-
- HY-168378A
-
|
S-2251 acetate
|
Fluorescent Dye
|
Others
|
|
D-Val-Leu-Lys-pNA acetate (S-2251 acetate) is a chromogenic peptide substrate that serves as a characteristic substrate for plasmin and plasminogen. D-Val-Leu-Lys-pNA acetate acts as a sensitive substrate for the DFE27 serine protease derived from Bacillus subtilis DC27. Catalyzed by plasmin, D-Val-Leu-Lys-pNA acetate binds and hydrolyzes to release p-nitroaniline (pNA), which can be detected colorimetrically at 405 nm as a measure of fibrinolytic activity .
|
-
- HY-D3152
-
|
|
Fluorescent Dye
|
Others
|
|
NRh-Cu is a highly sensitive near-infrared fluorescence turn-on indicator and Cu 2+-selective probe (λex=680 nm, λem=740 nm). When NRh-Cu interacts with Cu 2+, it undergoes a structural transition from a non-fluorescent spirocyclic state to a fluorescent ring-opened state, which drives the enhancement of fluorescence emission. NRh-Cu can be used for Cu 2+ imaging in live cells and in vivo .
|
-
| Cat. No. |
Product Name |
Type |
-
- HY-15924
-
Thiazolyl Blue
Maximum Cited Publications
182 Publications Verification
MTT; Thiazolyl Blue Tetrazolium bromide; Methylthiazolyldiphenyl-tetrazolium bromide
|
Fluorescent Dye
|
|
Thiazolyl Blue (MTT) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis .
|
-
- HY-137805
-
|
|
Fluorescent Dye
|
|
Ferrozine is a spectrophotometric reagent for iron ions, can react with divalent Fe to form a stable magenta complex species. The complex has an absorption peak at 562 nm . Ferrozine-based colorimetric assays can quantify iron in cells
|
-
- HY-77962
-
|
|
Fluorescent Dye
|
|
2-Thiobarbituric acid is a commonly used colorimetric reagent for the detection of malondialdehyde (MDA), a marker of lipid peroxidation. 2-Thiobarbituric acid forms a complex with MDA that can be quantified by colorimetric detection at 532 nm as a measure of lipid peroxidation .
|
-
- HY-D1224
-
|
CPRG
|
Fluorescent Dye
|
|
Chlorophenol red-β-D-galactopyranoside (CPRG) is an efficient and sensitive chromogenic substrate for β-galactosidase (HY-P2869), widely used in colorimetric assays. Chlorophenol red-β-D-galactopyranoside itself appears pale yellow. When it is specifically hydrolyzed by β-galactosidase, it releases chlorophenol red. The released chlorophenol red turns purple-red under alkaline or neutral pH conditions. This color change from yellow to red can be quantitatively detected at wavelengths of 540-572 nm using visible spectrophotometry .
|
-
- HY-15909
-
|
BCIP p-toluidine salt; X-phosphate p-toluidine salt
|
Fluorescent Dye
|
|
BCIP(BCIP p-toluidine salt; X-phosphate p-toluidine salt) is an artificial chromogenic substrate used for the sensitive colorimetric detection of alkaline phosphatase activity .
|
-
- HY-W013168
-
|
4-Nitrophenyl hexadecanoate; p-Nitrophenyl Palmitate; pNpp
|
Fluorescent Dye
|
|
4-Nitrophenyl palmitate (4-Nitrophenyl hexadecanoate) is a chromogenic substrate for lipases and esterases. Upon enzymatic hydrolysis, 4-Nitrophenyl palmitate releases p-nitrophenol, which can be quantified by colorimetric detection at 410 nm as a measure of enzymatic activity. 4-Nitrophenyl palmitate is used to characterize the activity of various bacterial and mammalian enzymes, including those from Burkholderia and porcine pancreatic lipase .
|
-
- HY-D0221
-
|
NSC 112125; 2,4,6-Tipyidyl-s-tiazie
|
Fluorescent Dye
|
|
2,4,6-Tri-2-pyridinyl-1,3,5-triazine (NSC 112125) is a colorimetric reagent for detecting iron. 2,4,6-Tri-2-pyridinyl-1,3,5-triazine forms a complex with Fe (II) and can be quantified as a measure of iron concentration by colorimetric detection at 594 nm .
|
-
- HY-125815
-
|
|
Fluorescent Dye
|
|
Reactive Blue 4 is an anthraquinone dye used extensively in the textile industry. Reactive Blue 4 is a single colorimetric chemosensor for sequential determination of multiple analytes with different optical responses in aqueous media .
|
-
- HY-151644
-
|
|
Fluorescent Dye
|
|
Fluorescein hydrazide is a sensitive fluorescent probe for Hg 2+ and Co 2+ that induces obvious color changes and fluorescence changes. Fluorescein hydrazide can be prepared by the reaction of fluorescein and hydrazine. Fluorescein hydrazide exhibits an excitation wavelength of 508 nM and an emission wavelength of 531 nM [2][3].
|
-
- HY-131409
-
|
D-Val-Leu-Lys-p-itoailide dihydrochloride; D-VLK-pNA dihydrochloride; S 2251 dihydrochloride
|
Fluorescent Dye
|
|
D-Val-Leu-Lys-pNA (D-Val-Leu-Lys-p-nitroanilide) dihydrochloride is a chromogenic peptide substrate that serves as a characteristic substrate for plasmin and plasminogen. D-Val-Leu-Lys-pNA dihydrochloride acts as a sensitive substrate for the DFE27 serine protease derived from Bacillus subtilis DC27. Catalyzed by plasmin, D-Val-Leu-Lys-pNA dihydrochloride binds and hydrolyzes to release p-nitroaniline (pNA), which can be detected colorimetrically at 405 nm as a measure of fibrinolytic activity .
|
-
- HY-P3236
-
|
Ac-IEPD-pNA
|
Fluorescent Dye
|
|
N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide (Ac-IEPD-pNA) is a colorimetric peptide substrate of granzyme B that allows accurate measurement of granzyme B activity .
|
-
- HY-D0202
-
|
|
Fluorescent Dye
|
|
Arsenazo III is an azo derivative of chromotropic acid, a metal chrome dye, a chelating agent, and a cation complexing agent. Arsenazo III forms stable 1:1 complexes with Ca 2+, Mg 2+, Sr 2+, Ba 2+, K +, and Na +, and its binding affinity for Ca 2+ depends on pH, alkali metal cation concentration, and buffer parameters. Arsenazo III serves as a colorimetric indicator for micromolar ionized Ca 2+ in cells .
|
-
- HY-D1599
-
|
R6GH
|
Fluorescent Dye
|
|
Rhodamine 6G hydrazide (R6GH) is a fluorescent dye. Rhodamine 6G hydrazide can be used in selective colorimetric and fluorescent sensing .
|
-
- HY-D0943
-
|
Kernechtrot
|
Fluorescent Dye
|
|
Nuclear Fast Red, an anthraquinone dye, is commonly used in conjunction with an excess of aluminum ions as a red nuclear counterstain. Nuclear fast red has been used as a histochemical and colorimetric reagent for calcium . Nuclear fast red as highly sensitive "off/on" fluorescent probe for detecting guanine .
|
-
- HY-D2329
-
|
|
Fluorescent Dye
|
|
Fluorescein-PEG4-NHS ester is a colorimetric probe formed by the reaction of adipic dihydrazide and fluorescein isothiocyanate .
|
-
- HY-D0813
-
|
|
Fluorescent Dye
|
|
Zincon sodium is an excellent colorimetric reagent for the detection of zinc and copper ions in aqueous solution. Zincon sodium can be used for the determination of zinc, copper, and cobalt ions in metalloproteins .
|
-
- HY-107864
-
|
Tetraiodofluorescein
|
Fluorescent Dye
|
|
Erythrosine B free acid is a visibly red dye with colorimetric and fluorescent properties that serves as an important dye for many Gram-positive and -negative bacteria. Erythrosine B free acid can be used for live/dead determination in both colorimetric and fluorescence-based assays for low, medium and high-throughput experimentation .
|
-
- HY-121694
-
|
|
Fluorescent Dye
|
|
CENTA is a colorimetric cephalosporin substrate for β-lactamases. Upon hydrolysis by β-lacatamases, CENTA turns from light yellow to chrome yellow, which can be quantified by colorimetric detection at 405 nm as a measure of β-lactamase activity.
|
-
- HY-D0108
-
|
|
Fluorescent Dye
|
|
Fluorescein dilaurate is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.
|
-
- HY-D0374
-
|
|
Fluorescent Dye
|
|
Reactive Blue 4 sodium is an anthraquinone dye used extensively in the textile industry. Reactive Blue 4 sodium is a single colorimetric chemosensor for sequential determination of multiple analytes with different optical responses in aqueous media .
|
-
- HY-W142631
-
|
|
Fluorescent Dye
|
|
4-(Phenylazo)diphenylamine is an excellent colorimetric indicator for the accurate determination of the concentration for a variety of strong bases, Lewis acids, and hydride reducing agents .
|
-
- HY-137858
-
|
Gly-Arg-4-NA hydrochloride; GR-pNA hydrochloride; GR p-itoailide hydrochloride
|
Fluorescent Dye
|
|
H-Gly-Arg-pNA hydrochloride (GR-pNA hydrochloride) is a colorimetric substrate for thrombin. H-Gly-Arg-pNA is preferentially bound by thrombin and cleaves the Gly-Arg (GR) peptide sequence, releasing p-nitroaniline (pNA) that can be detected colorimetrically at 405 nm. H-Gly-Arg-pNA hydrochloride is used as a fluorescent dye to measure thrombin activity .
|
-
- HY-D0107
-
|
|
Fluorescent Dye
|
|
Fluorescein dicaproate is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.
|
-
- HY-D0101
-
|
|
Fluorescent Dye
|
|
Fluorescein-diphosphat (diammonium) is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.
|
-
- HY-D0105
-
|
|
Fluorescent Dye
|
|
Fluorescein diacetate 6-isothiocyanate is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.
|
-
- HY-D0103
-
|
|
Fluorescent Dye
|
|
Fluorescein diacetate 5-maleimide is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.
|
-
- HY-D0100
-
|
|
Fluorescent Dye
|
|
Fluorescein-diacetate-5-isothiocyanat is a colorimetric probe formed by the reaction of adipic acid dihydrazide and fluorescein isothiocyanate.
|
-
- HY-W011422
-
|
NSC 528
|
Fluorescent Dye
|
|
2,6-Dibromo-4-(chloroimino)cyclohexa-2,5-dienone (NSC 528) is a colorimetric dye used for the detection of phenolic compounds. 2,6-Dibromo-4-(chloroimino)cyclohexa-2,5-dienone reacts with phenolic compounds at a pH of about 9.4 to produce an indigo dye. 2,6-Dibromo-4-(chloroimino)cyclohexa-2,5-dienone has been used as a Gibbs reagent for the colorimetric detection of phenolic compounds. 2,6-Dibromo-4-(chloroimino)cyclohexa-2,5-dienone has also been used in a colorimetric reaction for the quantitative colorimetric detection of aflatoxins, turning the colorless aflatoxins green with an absorption band at 673 nm.
|
-
- HY-W009458
-
|
3-IP sodium
|
Fluorescent Dye
|
|
3-Indoxyl phosphate (3-IP) is a chromogenic and electrochemical substrate for alkaline phosphatase. Upon enzymatic cleavage by alkaline phosphatase, 3-indoxyl is released and subsequently oxidized to produce water-insoluble indigo, which can be quantified by colorimetric detection at 660 nm as a measure of alkaline phosphatase activity. 3-Indoxyl phosphate can also be converted to water-soluble leucoindigo by reduction of indigo in alkaline solution by sodium dithionite, which can be quantified by colorimetric detection at 415 nm or voltametric detection as a measure of alkaline phosphatase activity.2 3-Indoxyl phosphate has been used in ELISAs.
|
-
- HY-145499
-
|
|
Fluorescent Dye
|
|
XTT is a cell-impermeable, negatively charged tetrazolium dye that produces a water-soluble formazan when reduced at the cell surface by cellular-derived NADH and an electron mediator. It is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis.
|
-
- HY-120971
-
|
DepNA
|
Fluorescent Dye
|
|
N-Decanoyl p-nitroaniline (DepNA) is one of several nitroaniline fatty acid amides which can be used to measure fatty acid amide hydrolase (FAAH) activity.1 FAAH is a relatively unselective enzyme in that it accepts a variety of amide head groups other than the ethanolamine of its endogenous substrate anandamide (AEA). It also will hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate. Exposure of DepNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε=13,500 at 410 nm). This allows the fast and convenient measurement of FAAH activity using a 96 well plate spectrophotometer.
|
-
- HY-107864R
-
|
Tetraiodofluorescein (Standard)
|
Fluorescent Dye
|
|
Erythrosine B free acid (Standard) is the analytical standard of Erythrosine B (free acid) (HY-107864). This product is intended for research and analytical applications. Erythrosine B free acid is a visibly red dye with colorimetric and fluorescent properties that serves as an important dye for many Gram-positive and -negative bacteria. Erythrosine B free acid can be used for live/dead determination in both colorimetric and fluorescence-based assays for low, medium and high-throughput experimentation .
|
-
- HY-D2969
-
|
|
Fluorescent Dye
|
|
CYDA is a colorimetric probe based on cyanine dyes, which enables highly sensitive and selective detection of Cu²⁺. CYDA can be used for urine copper detection in Wilson's disease .
|
-
- HY-DY1106
-
|
|
Fluorescent Dye
|
Ferrozine (solution) is a spectrophotometric reagent for iron ions, can react with divalent Fe to form a stable magenta complex species. The complex has an absorption peak at 562 nm . Ferrozine-based colorimetric assays can quantify iron in cells Solvent and concentration: ddH2O: 10 mM
|
-
- HY-DY1086
-
|
MTT (solution); Thiazolyl Blue Tetrazolium bromide (solution); Methylthiazolyldiphenyl-tetrazolium bromide (solution)
|
Fluorescent Dye
|
Thiazolyl Blue (solution) (MTT (solution)) is a cell-permeable and positively charged tetrazolium dye that is used to detect reductive metabolism in cells. Thiazolyl Blue is taken up by cells through the plasma membrane and then reduced to formazan by intracellular NAD (P) H-oxidoreductases. Thiazolyl Blue is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis . Solvent and concentration: PBS: 5 mg/mL
|
-
- HY-D3244
-
|
|
Fluorescent Dye
|
|
RDDB is a rhodamine-based turn-on fluorescent and colorimetric chemosensor selective for Mn 2+, with a limit of detection of 5×10 -8 M (excitation wavelength: 480-550 nm; fluorescence signal collection range: >590 nm). In the presence of Mn 2+, RDDB undergoes a spirolactam ring-opening reaction of its rhodamine hydrazide moiety, resulting in turn-on fluorescence and a color change. RDDB can be used for intracellular Mn 2+ imaging .
|
-
- HY-D3152
-
|
|
Fluorescent Dye
|
|
NRh-Cu is a highly sensitive near-infrared fluorescence turn-on indicator and Cu 2+-selective probe (λex=680 nm, λem=740 nm). When NRh-Cu interacts with Cu 2+, it undergoes a structural transition from a non-fluorescent spirocyclic state to a fluorescent ring-opened state, which drives the enhancement of fluorescence emission. NRh-Cu can be used for Cu 2+ imaging in live cells and in vivo .
|
| Cat. No. |
Product Name |
Type |
-
- HY-W034953
-
|
|
Biochemical Assay Reagents
|
|
Bathocuproine disulfonate disodium (BCS) is an organic compound used as a highly sensitive colorimetric reagent for copper ions in biochemical and analytical applications. It has a bright yellow color and absorbs light at specific wavelengths, so it can be used to detect and quantify trace copper. In biochemical applications, BCS is commonly used to study the role of copper ions in various biological processes. Copper is an essential nutrient for many organisms, but it can also be toxic in high concentrations, so accurate measurement of copper levels is important to understand its impact on living systems. In terms of analysis, BCS is often used in environmental monitoring and water quality testing to detect copper pollution. It can detect copper even at very low concentrations, making it an invaluable tool for identifying potential sources of pollution and assessing the impact of industrial activities on aquatic ecosystems.
|
-
- HY-W009694
-
|
|
Biochemical Assay Reagents
|
|
3,5-Dinitrosalicylic acid the derivative of salicylic acid. 3,5-Dinitrosalicylic acid is used in the α-amylase assay, carbohydrase assay, and for the colorimetric determination of reducing substances .
|
-
- HY-15926
-
ONPG
Maximum Cited Publications
6 Publications Verification
2-Nitrophenyl β-D-galactopyranoside
|
Biochemical Assay Reagents
|
|
ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity.
|
-
- HY-15920
-
INT
3 Publications Verification
Iodonitrotetrazolium chloride; p-Iodonitrotetrazolium Violet
|
Biochemical Assay Reagents
|
|
INT (Iodonitrotetrazolium chloride) is a biochemical reagent mainly used in the iodonitrotetrazolium chloride (INT) colorimetric method to assess the metabolic activity of cells and the activity of enzymes .
|
-
- HY-W129633
-
|
DCIP sodium hydrate; DPIP sodium hydrate
|
Biochemical Assay Reagents
|
|
2,6-Dichlorophenolindophenol (DCIP; DPIP) sodium hydrate is a redox chromogenic indicator with a redox potential of +217 mV relative to SHE, and it acts as a substrate for reduction reactions. During the metabolic process of nutrient consumption by Saccharomyces cerevisiae, 2,6-Dichlorophenolindophenol sodium hydrate is reduced from dark blue to colorless, resulting in a decrease in absorbance. 2,6-Dichlorophenolindophenol sodium hydrate is widely used in spectrophotometric biochemical oxygen demand determination and preclinical colorimetric toxicity analysis for heavy metal ion detection based on Saccharomyces cerevisiae .
|
-
- HY-Y0699
-
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Biochemical Assay Reagents
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Methyl red is a pH-sensitive acid-base indicator and colorimetric reagent with a pKa of 5.1. Methyl red achieves visual judgment of the titration endpoint through reversible structural changes of protonation (red, pH <4.4) and deprotonation (yellow, pH >6.2). Methyl red is widely used in acid-base titration in chemical analysis and buffer pH monitoring in biochemistry .
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- HY-W011654
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4-Aminophenyl-beta-D-galactopyranoside, 98%
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Biochemical Assay Reagents
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4-Aminophenyl-β-D-galactopyranoside, 98% is a highly efficient substrate for β-galactosidase. It is specifically hydrolyzed by this enzyme to release galactose and electroactive p-aminophenol. 4-Aminophenyl-β-D-galactopyranoside, 98% is widely used in colorimetric and electrochemical assays for detecting β-galactosidase activity and determining enzyme kinetics, such as in biosensing fields including cellular senescence, pathogen and contaminant detection. In addition, since β-galactosidase is often overexpressed in primary ovarian cancer, 4-Aminophenyl-β-D-galactopyranoside, 98% can also be applied to related research on primary ovarian cancer .
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- HY-15929
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TBHBA
1 Publications Verification
2,4,6-Tribromo-3-hydroxybenzoic acid
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Biochemical Assay Reagents
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TBHBA (2,4,6-Tribromo-3-hydroxybenzoic acid) is a chemical colorimetric agent and nanomaterial surface modifier. TBHBA can react with some oxidants (such as H2O2) to generate high-absorbance quinoneimine dyes, which enhance the sensitivity of HDL cholesterol enzymatic determination. TBHBA can be used as a bromine-containing coupling agent to modify the surface of silica nanoparticles through esterification reaction. Thereby, organic flame retardant groups are introduced to improve the thermal stability and flame retardant properties of nanocomposites. TBHBA can be used in clinical biochemical testing (such as serum lipoprotein analysis) and the preparation of flame-retardant polymer nanocomposites .
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- HY-W110793
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Diphenylthiocarbazone
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Biochemical Assay Reagents
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Dithizone can be toxic to pancreatic islet cells and cause experimental diabetes. Dithizone can be used for in vitro in vivo staining of transplanted islets and cytoplasmic granules of bone marrow cells. Dithizone can be used as a chelating agent and colorimetric indicator for the detection and extraction of various heavy metals, including lead and mercury, in analytical and biochemical applications. Dithizone forms stable colored complexes with metal ions and can be quantitatively analyzed by methods such as spectrophotometry .
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- HY-W127790
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Biochemical Assay Reagents
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Bathophenanthrolinedisulfonic acid disodium salt (BPS) is a colorimetric reagent for iron detection, with a wavelength of 535 nm for iron complex. It can be used for iron determination in aqueous solution systems without the need for extraction solvents. BPS can serve as a precursor to prepare Europium(III) tris(dibenzoylmethanate) bis(bathophenanthrolinedisulfonate) complex and ruthenium(II) tris(bathophenanthrolinedisulfonate), or participate in the catalytic oxidation of 2-hexanol by forming water-soluble complexes with palladium .
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- HY-W009311
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4,4'-Bis(dimethylamino)thiobenzophenone
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Biochemical Assay Reagents
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Thiomichler's ketone (4,4'-Bis (dimethylamino) thiobenzophenone) is a heavy metal complexing agent and a colorimetric/spectrophotometric reagent. Thiomichler's ketone shows selectivity for Hg 2+ at pH 3, and exhibits activity towards Pd 2+ and Ag + at pH 3.5. Thiomichler's ketone enables accurate detection of trace Pd 2+ by forming an extractable Hg 2+ complex via micelle-mediated cloud point extraction, or generating a red coordination complex including Pd (TMK)4. Thiomichler's ketone is applicable for the determination of trace Pd 2+ in antibiotics and catalysts of automobile exhaust purifiers .
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- HY-D1224A
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CPRG sodium
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Biochemical Assay Reagents
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Chlorophenol red-β-D-galactopyranoside (CPRG) sodium is an efficient and sensitive chromogenic substrate for β-galactosidase (HY-P2869), widely used in colorimetric assays. Chlorophenol red-β-D-galactopyranoside sodium itself appears pale yellow. When it is specifically hydrolyzed by β-galactosidase, it releases chlorophenol red. The released chlorophenol red turns purple-red under alkaline or neutral pH conditions. This color change from yellow to red can be quantitatively detected at wavelengths of 540-572 nm using visible spectrophotometry .
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- HY-165025
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- HY-W129500
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Biochemical Assay Reagents
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N-[[Bis[4-(dimethylamino)phenyl]amino]carbonyl]glycine sodium can be used as a biomonitoring reagent to measure redox activity through a colorimetric reaction.
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- HY-157926
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Biochemical Assay Reagents
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Nitroso-PSAP is a sensitive and specific chromogenic reagent for iron (Fe 2+), which is used for the colorimetric determination of iron in blood after mineralization with periodic acid. TNitroso-PSAP chelates with iron (Fe 2+) to produce a green complex (λmax=756 nm, Epsilon = 4.5 x 10 < sup > 4 < / sup > dm < sup > 3 < / sup > mol < sup > 1 < / sup > cm < sup > 1 < / sup >) .
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- HY-171819
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Biochemical Assay Reagents
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O157 Medium powder can be used to prepare a colorimetric medium for the separation and differentiation of Escherichia coli O157.
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| Cat. No. |
Product Name |
Target |
Research Area |
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- HY-131409
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D-Val-Leu-Lys-p-itoailide dihydrochloride; D-VLK-pNA dihydrochloride; S 2251 dihydrochloride
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Fluorescent Dye
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Others
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D-Val-Leu-Lys-pNA (D-Val-Leu-Lys-p-nitroanilide) dihydrochloride is a chromogenic peptide substrate that serves as a characteristic substrate for plasmin and plasminogen. D-Val-Leu-Lys-pNA dihydrochloride acts as a sensitive substrate for the DFE27 serine protease derived from Bacillus subtilis DC27. Catalyzed by plasmin, D-Val-Leu-Lys-pNA dihydrochloride binds and hydrolyzes to release p-nitroaniline (pNA), which can be detected colorimetrically at 405 nm as a measure of fibrinolytic activity .
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- HY-P3236
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Ac-IEPD-pNA
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Ser/Thr Protease
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Cancer
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N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide (Ac-IEPD-pNA) is a colorimetric peptide substrate of granzyme B that allows accurate measurement of granzyme B activity .
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- HY-P5348
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Peptides
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Others
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Ac-LEHD-pNA is a biological active peptide. (Caspase-9 substrate; pNA (4-nitroaniline)-derived caspase substrates are widely used for the colorimetric detection of various caspase activities. Cleavage of pNA peptides by caspases generates pNA that is monitored colorimetrically at ~405 nm. pNA has maximum absorption around 408 nm.)
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- HY-120833
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Caspase
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Others
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Ac-Ile-Glu-Thr-Asp-pNA is a substrate for caspase-8. Caspase-8 binds to and cleaves the Ile-Glu-Thr-Asp (IETD) peptide sequence to release p-nitroalinide, which can be quantified by colorimetric detection at 405 nm as a measure of enzyme activity .
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- HY-P10236
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Caspase
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Others
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Ac-WEHD-pNA is a colorimetric substrate for caspase 1, 4, 5 and 14, which is utilized to measure the caspase activity .
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- HY-126785
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Colorimetric Elastase Substrate
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Elastase
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Others
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Suc-AAP-Abu-pNA (Colorimetric Elastase Substrate) is a specific substrate for pancreatic elastase (Km = 100 μM; Kcat/Km = 35,300 s -1 M -1 for rat pancreatic elastase; Km = 30 μM; Kcat/Km = 351,000 s -1 M -1 for porcine pancreatic elastase). Suc-AAP-Abu-pNA also promotes OPC migration .
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- HY-P5992
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Peptides
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Others
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Factor VIIa substrate is a colorimetric substrate for Factor VIIa, that can be measured by absorbance at 405 nm .
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- HY-P4335
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Ser/Thr Protease
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Others
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Bz-RGFFP-4MβNA, a substrate for Cathepsin D, is suitable for either colorimetric or fluorometric assay .
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- HY-P10001
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Z-Ile-Glu-Thr-Asp-pNA
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Caspase
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Cancer
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Z-IETD-pNA (Z-Ile-Glu-Thr-Asp-pNA) is a colorimetric caspase-8 and granzyme B substrate. Z-IETD-pNA is hydrlyzed by caspase 8 to generate pNA .
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- HY-P10059
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Peptides
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Others
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Boc-Val-Gly-Arg-βNA is a colorimetric substrate for plasminogen activator .
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- HY-168378A
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S-2251 acetate
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Fluorescent Dye
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Others
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D-Val-Leu-Lys-pNA acetate (S-2251 acetate) is a chromogenic peptide substrate that serves as a characteristic substrate for plasmin and plasminogen. D-Val-Leu-Lys-pNA acetate acts as a sensitive substrate for the DFE27 serine protease derived from Bacillus subtilis DC27. Catalyzed by plasmin, D-Val-Leu-Lys-pNA acetate binds and hydrolyzes to release p-nitroaniline (pNA), which can be detected colorimetrically at 405 nm as a measure of fibrinolytic activity .
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- HY-K0325
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MCE Total Superoxide Dismutase (SOD) Activity Colorimetric Assay Kit (WST-8 Method) employs a water-soluble tetrazolium salt (WST-8)–based colorimetric system and quantifies total SOD activity in various biological samples by measuring the inhibition of WST-8 reduction to water-soluble formazan in the xanthine/xanthine oxidase system at 450 nm.
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- HY-K2002
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1 Publications Verification
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MCE BCA Protein Assay Kit is a colorimetric assay based on the bicinchoninic acid (BCA) reaction principle for quantitative protein determination.
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- HY-K1501
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2 Publications Verification
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MCE NADP+/NADPH Assay Kit (WST-8) is a colorimetric assay that conveniently detects the oxidized (NADP+) and reduced (NADPH) forms of coenzyme II within cells, tissues, and other samples.
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- HY-K0313
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2 Publications Verification
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MCE NAD+/NADH Assay Kit (WST-8) is a colorimetric assay that conveniently detects the oxidized (NAD+) and reduced (NADH) forms of coenzyme Ⅰ within cells, tissues, and other samples.
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- HY-K0301
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Maximum Cited Publications
3907 Publications Verification
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Cell Counting Kit-8 (CCK-8) allows sensitive colorimetric assays for the determination of cell viability in cell proliferation and cytotoxicity assays.The 5 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
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- HY-K0324
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MCE MCE Total Superoxide Dismutase (SOD) Activity Colorimetric Assay Kit (Xanthine Oxidase-NBT Method) is based on the xanthine oxidase–NBT chromogenic system and quantitatively determines total SOD activity in various biological samples by measuring the inhibition of NBT reduction to formazan in the xanthine/xanthine oxidase–generated superoxide system at 560 nm. It is suitable for the determination of SOD activity in cell or tissue homogenate supernatants, whole blood, erythrocyte extracts, serum, and other biological samples.
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| Cat. No. |
Product Name |
Category |
Target |
Chemical Structure |
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- HY-B1306
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p-Aminohippuric acid
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Classification of Application Fields
Ketones, Aldehydes, Acids
Other Diseases
Endogenous metabolite
Disease Research Fields
Source Classification
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Others
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4-Aminohippuric acid (p-Aminohippuric acid) is a coordination ligand for metal ions (such as Cu 2+, Fe 3+, Hg 2+) and a functionalization reagent for nanomaterials. 4-Aminohippuric acid can coordinate with metal ions or modify the surface of materials such as carbon nanotubes and gold nanoparticles through amino and carboxyl groups. 4-Aminohippuric acid can form stable complexes with metal ions or participate in the synthesis of nanomaterials as a reducing agent/stabilizer, enriching metal ions or giving nanoparticles peroxidase-mimicking activity. 4-Aminohippuric acid can be used to construct highly sensitive electrochemical sensors or colorimetric sensors to detect and quantitatively analyze heavy metal ions such as copper, iron, and mercury in environmental water samples and biological samples. 4-Aminohippuric acid may also be a biomarker for attention-deficit/hyperactivity disorder (ADHD) .
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- HY-B1306R
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p-Aminohippuric acid (Standard)
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Structural Classification
Ketones, Aldehydes, Acids
Endogenous metabolite
Source Classification
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Reference Standards
Biochemical Assay Reagents
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4-Aminohippuric acid (p-Aminohippuric acid) (Standard) is the analytical standard of 4-Aminohippuric acid (HY-B1306). This product is intended for research and analytical applications. 4-Aminohippuric acid (p-Aminohippuric acid) is a coordination ligand for metal ions (such as Cu 2+, Fe 3+, Hg 2+) and a functionalization reagent for nanomaterials. 4-Aminohippuric acid can coordinate with metal ions or modify the surface of materials such as carbon nanotubes and gold nanoparticles through amino and carboxyl groups. 4-Aminohippuric acid can form stable complexes with metal ions or participate in the synthesis of nanomaterials as a reducing agent/stabilizer, enriching metal ions or giving nanoparticles peroxidase-mimicking activity. 4-Aminohippuric acid can be used to construct highly sensitive electrochemical sensors or colorimetric sensors to detect and quantitatively analyze heavy metal ions such as copper, iron, and mercury in environmental water samples and biological samples. 4-Aminohippuric acid may also be a biomarker for attention-deficit/hyperactivity disorder (ADHD) .
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| Cat. No. |
Product Name |
Chemical Structure |
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- HY-15926S
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ONPG- 13C is the 13C labeled ONPG. ONPG is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activit .
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- HY-B1306S
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4-Aminohippuric acid-d4 (p-Aminohippuric acid-d4) is the deuterium labeled 4-Aminohippuric acid (HY-B1306). 4-Aminohippuric acid (p-Aminohippuric acid) is a coordination ligand for metal ions (such as Cu 2+, Fe 3+, Hg 2+) and a functionalization reagent for nanomaterials. 4-Aminohippuric acid can coordinate with metal ions or modify the surface of materials such as carbon nanotubes and gold nanoparticles through amino and carboxyl groups. 4-Aminohippuric acid can form stable complexes with metal ions or participate in the synthesis of nanomaterials as a reducing agent/stabilizer, enriching metal ions or giving nanoparticles peroxidase-mimicking activity. 4-Aminohippuric acid can be used to construct highly sensitive electrochemical sensors or colorimetric sensors to detect and quantitatively analyze heavy metal ions such as copper, iron, and mercury in environmental water samples and biological samples. 4-Aminohippuric acid may also be a biomarker for attention-deficit/hyperactivity disorder (ADHD) .
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