2. Metabolic Enzyme/Protease
  3. Dopamine β-hydroxylase
  4. Cuprizone

Cuprizone is a copper chelating agent that forms a deep blue copper ketone complex with copper (II). The copper ketone reaction can be used in colorimetric tests for the presence of trace copper. Cuprizone can be used to induce some schizophrenia-like behavior in mice. Cuprizone acts on copper enzymes, including SOD1, cytochrome oxidase, and DβH, thereby causing oxidative stress and increasing DA levels in certain brain regions such as the medial prefrontal cortex (PFC).

For research use only. We do not sell to patients.

CAS No. : 370-81-0

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Cuprizone:

Cuprizone Related Antibodies

Top Publications Citing Use of Products
WB
In Vivo Efficacy Study
IF
Histological Imaging/Staining

    Cuprizone purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2023 Apr;44(4):766-779.  [Abstract]

    Cuprizone (CPZ) (0.3%; p.o.; 5 weeks) increased the expression of TRPV1 protein in the corpus callosum of C57BL/6 mice.

    Cuprizone purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2023 Apr;44(4):766-779.  [Abstract]

    Cuprizone (CPZ) (0.3%; p.o.; 5 weeks) caused significant body weight loss in C57BL/6 and Trpv1-/- mice.

    Cuprizone purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2023 Apr;44(4):766-779.  [Abstract]

    Cuprizone (CPZ) (0.3%; p.o.; 5 weeks) significantly decreased the protein levels of MBP in the corpus callosum of Trpv1-/- mice.

    Cuprizone purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2023 Apr;44(4):766-779.  [Abstract]

    Representative immunofluorescent images of MBP staining in the corpus callosum of Trpv1-/- mice treated with Cuprizone (CPZ) (0.3%; p.o.; 5 weeks).

    Cuprizone purchased from MedChemExpress. Usage Cited in: Acta Pharmacol Sin. 2023 Apr;44(4):766-779.  [Abstract]

    Cuprizone (CPZ) (0.3%; p.o.; 5 weeks) significantly decreased myelinated axons in C57BL/6 and Trpv1-/- mice.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Cuprizone is a copper chelating agent that forms a deep blue copper ketone complex with copper (II). The copper ketone reaction can be used in colorimetric tests for the presence of trace copper. Cuprizone can be used to induce some schizophrenia-like behavior in mice. Cuprizone acts on copper enzymes, including SOD1, cytochrome oxidase, and DβH, thereby causing oxidative stress and increasing DA levels in certain brain regions such as the medial prefrontal cortex (PFC)[1].

    In Vivo

    Cuprizone (0.5 mg/100 mg of diet and water given ad lib; p.o.) produces severe status spongious in the CNS, most prominently in the brain stem and cerebellar white matter of mice[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    278.35

    Formula

    C14H22N4O2
    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(C(N/N=C1CCCCC\1)=O)N/N=C2CCCCC/2
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 6.67 mg/mL (23.96 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.5926 mL 17.9630 mL 35.9260 mL
    5 mM 0.7185 mL 3.5926 mL 7.1852 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
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    Volume
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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

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    C2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 0.67 mg/mL (2.41 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.67 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 0.67 mg/mL (2.41 mM); Clear solution

      This protocol yields a clear solution of ≥ 0.67 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.7 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.61%

    SDS (761 KB)

    COA (245 KB) HNMR (246 KB) RP-HPLC (246 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.5926 mL 17.9630 mL 35.9260 mL 89.8150 mL
    5 mM 0.7185 mL 3.5926 mL 7.1852 mL 17.9630 mL
    10 mM 0.3593 mL 1.7963 mL 3.5926 mL 8.9815 mL
    15 mM 0.2395 mL 1.1975 mL 2.3951 mL 5.9877 mL
    20 mM 0.1796 mL 0.8981 mL 1.7963 mL 4.4907 mL
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    Cuprizone Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Cuprizone370-81-0Dopamine β-hydroxylaseDopamine beta-hydroxylaseDopamine beta-monooxygenaseDopamine β-monooxygenasecoordinate Copper(II)copper-cuprizone complex,antipsychoticsschizophrenia modelbehavioroligodendrocytesMBPmouseInhibitorinhibitorinhibit

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