Xylenol orange tetrasodium salt, IND
Based on 1 publication(s) in Google Scholar
Xylenol orange tetrasodium salt, IND is commonly used as a colorimetric indicator for metal titration and possesses Zn2+ chelating activity. Xylenol orange tetrasodium salt, IND binds Zn2+ under physiological pH, and its Zn-XO complex allows visual differentiation between tartrate and malate ions. Xylenol orange tetrasodium salt, IND undergoes decolorization upon oxidation by hydrogen peroxide, undergoes a Co (II)-catalyzed pseudo-second-order reaction in a strong alkaline environment, and regulates the ferrous ion oxidation process mediated by peroxy radicals. Xylenol orange tetrasodium salt, IND forms colored ternary complexes with various anions, cations and iron-alanine, which is suitable for spectrophotometric quantification and can also be used for alanine irradiation dose detection. Xylenol orange tetrasodium salt, IND is applicable to EDTA metal titration analysis under acidic conditions.
For research use only. We do not sell to patients.
- Purity : 95.0%
- CAS No.: 3618-43-7
- Formula: C31H28N2Na4O13S
- Molecular Weight:760.58
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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HY-D0040
Calcein -
HY-D0169
Phenol Red -
HY-D0041
Calcein-AM -
HY-D0012
Bromothymol Blue -
HY-B1571
Bromophenol blue -
HY-W040144
Bromocresol green -
HY-D0250
o-Cresolphthalein complexone -
HY-W087928
Bromocresol purple -
HY-D0236
Congo Red -
HY-Y0699
Methyl red -
HY-D0166
Neutral Red -
HY-W110910
Eriochrome black T, Indicator
Publications Citing Use of MedChemExpress (MCE) Xylenol orange tetrasodium salt, IND
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Biological Activity
Description
In Vitro
Xylenol orange tetrasodium salt, IND forms a thermodynamically stable dinuclear Zn2-XO complex in 20 mM MOPS buffer at pH 7.4, with an overall stability constant log β = 8.95, accompanied by a color change from red to purple and characteristic UV-Vis spectral shift[1].
Xylenol orange tetrasodium salt, IND forms a Zn-XO complex with Zn2+ in MOPS buffer, and this complex exhibits a characteristic color change from purple to golden yellow upon contact with tartrate or malate.
Xylenol orange tetrasodium salt, IND undergoes decolorization by hydrogen peroxide in cell-free systems that conforms to xylenol orange concentration-dependent pseudo-second-order kinetics, reaches maximum reactivity at pH 9.0, displays optimal activity under Co(II) catalysis, and has an activation energy of 113.78 kJ·mol-1[2].
Xylenol orange tetrasodium salt, IND forms a Fe3+-XO-alanine complex in cell-free spectrophotometric assays[3].
Xylenol orange tetrasodium salt, IND is applied in cell-free spectrophotometric alanine dosimetry, where the optimal Fe2+ concentration to oxidize 2 kGy-irradiated alanine is 2×10-4 mol·dm-3; distinct calibration curves linking radiation dose and Fe3+ concentration are obtained for reagents of different batches, with varying slopes and intercepts[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 3618-43-7
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Appearance Solid
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Molecular Weight 760.58
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Formula C31H28N2Na4O13S
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Color Brown to red
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SMILES
O=C(CN(CC1=C(O)C(C)=CC(C2(C3=CC(CN(CC(O[Na])=O)CC(O[Na])=O)=C(O)C(C)=C3)OS(=O)(C4=C2C=CC=C4)=O)=C1)CC(O[Na])=O)O[Na]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
In Vitro:
H2O : 20 mg/mL (26.30 mM; Need ultrasonic)
DMSO : 8.33 mg/mL (10.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (281 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.3148 mL | 6.5739 mL | 13.1479 mL | 32.8697 mL |
| 5 mM | 0.2630 mL | 1.3148 mL | 2.6296 mL | 6.5739 mL | |
| 10 mM | 0.1315 mL | 0.6574 mL | 1.3148 mL | 3.2870 mL | |
| H2O | 15 mM | 0.0877 mL | 0.4383 mL | 0.8765 mL | 2.1913 mL |
| 20 mM | 0.0657 mL | 0.3287 mL | 0.6574 mL | 1.6435 mL | |
| 25 mM | 0.0526 mL | 0.2630 mL | 0.5259 mL | 1.3148 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Xylenol orange tetrasodium salt, IND Related Classifications
HY-W110883 Related Classifications
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